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Title: Impact of Amorphous SiO{sub 2} Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications

Abstract

It is generally accepted that silica (SiO{sub 2}) is not toxic. But the increasing use of silica nanoparticles (SiO{sub 2}NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO{sub 2}NPs on animal and cell physiology. Stable and monodisperse amorphous silica nanoparticles, 25 nM in diameter, were administered to living Hydra vulgaris (Cnidaria). The dose-related effects were defined by morphological and behavioral assays. The results revealed an all-or-nothing lethal toxicity with a rather high threshold (35 nM NPs) and a LT50 of 38 h. At sub lethal doses, the morphophysiological effects included: animal morphology alterations, paralysis of the gastric region, disorganization and depletion of tentacle specialized cells, increase of apoptotic and collapsed cells, and reduction of the epithelial cell proliferation rate. Transcriptome analysis (RNAseq) revealed 45 differentially expressed genes, mostly involved in stress response and cuticle renovation. Our results show that Hydra reacts to SiO{sub 2}NPs, is able to rebalance the animal homeostasis up to a relatively high doses of SiO{sub 2}NPs, and that the physiological modifications are transduced to gene expression modulation.

Authors:
;  [1];  [2]; ;  [3];  [1];  [2]; ;  [1]
  1. Istituto di Cibernetica “Eduardo Caianiello”, Consiglio Nazionale delle Ricerche, Pozzuoli (Italy)
  2. Center for Biomolecular Nanotechnologies@UNILE, Istituto Italiano di Tecnologia, Arnesano (Italy)
  3. The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan (Israel)
Publication Date:
OSTI Identifier:
22688012
Resource Type:
Journal Article
Journal Name:
Frontiers in Bioengineering and Biotechnology
Additional Journal Information:
Journal Volume: 2; Other Information: Copyright (c) 2014 Ambrosone, Scotto di Vettimo, Malvindi, Roopin, Levy, Marchesano, Pompa, Tortiglione and Tino.; This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 2296-4185
Country of Publication:
Switzerland
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; LETHAL DOSES; MOLECULAR BIOLOGY; MORPHOLOGY; NANOPARTICLES; SILICON OXIDES; TOXICITY

Citation Formats

Ambrosone, Alfredo, Scotto di Vettimo, Maria Rosaria, Malvindi, Maria Ada, Roopin, Modi, Levy, Oren, Marchesano, Valentina, Pompa, Pier Paolo, Tortiglione, Claudia, and Tino, Angela, E-mail: a.tino@cib.na.cnr.it. Impact of Amorphous SiO{sub 2} Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications. Switzerland: N. p., 2014. Web. doi:10.3389/FBIOE.2014.00037.
Ambrosone, Alfredo, Scotto di Vettimo, Maria Rosaria, Malvindi, Maria Ada, Roopin, Modi, Levy, Oren, Marchesano, Valentina, Pompa, Pier Paolo, Tortiglione, Claudia, & Tino, Angela, E-mail: a.tino@cib.na.cnr.it. Impact of Amorphous SiO{sub 2} Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications. Switzerland. doi:10.3389/FBIOE.2014.00037.
Ambrosone, Alfredo, Scotto di Vettimo, Maria Rosaria, Malvindi, Maria Ada, Roopin, Modi, Levy, Oren, Marchesano, Valentina, Pompa, Pier Paolo, Tortiglione, Claudia, and Tino, Angela, E-mail: a.tino@cib.na.cnr.it. Mon . "Impact of Amorphous SiO{sub 2} Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications". Switzerland. doi:10.3389/FBIOE.2014.00037.
@article{osti_22688012,
title = {Impact of Amorphous SiO{sub 2} Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications},
author = {Ambrosone, Alfredo and Scotto di Vettimo, Maria Rosaria and Malvindi, Maria Ada and Roopin, Modi and Levy, Oren and Marchesano, Valentina and Pompa, Pier Paolo and Tortiglione, Claudia and Tino, Angela, E-mail: a.tino@cib.na.cnr.it},
abstractNote = {It is generally accepted that silica (SiO{sub 2}) is not toxic. But the increasing use of silica nanoparticles (SiO{sub 2}NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO{sub 2}NPs on animal and cell physiology. Stable and monodisperse amorphous silica nanoparticles, 25 nM in diameter, were administered to living Hydra vulgaris (Cnidaria). The dose-related effects were defined by morphological and behavioral assays. The results revealed an all-or-nothing lethal toxicity with a rather high threshold (35 nM NPs) and a LT50 of 38 h. At sub lethal doses, the morphophysiological effects included: animal morphology alterations, paralysis of the gastric region, disorganization and depletion of tentacle specialized cells, increase of apoptotic and collapsed cells, and reduction of the epithelial cell proliferation rate. Transcriptome analysis (RNAseq) revealed 45 differentially expressed genes, mostly involved in stress response and cuticle renovation. Our results show that Hydra reacts to SiO{sub 2}NPs, is able to rebalance the animal homeostasis up to a relatively high doses of SiO{sub 2}NPs, and that the physiological modifications are transduced to gene expression modulation.},
doi = {10.3389/FBIOE.2014.00037},
journal = {Frontiers in Bioengineering and Biotechnology},
issn = {2296-4185},
number = ,
volume = 2,
place = {Switzerland},
year = {2014},
month = {9}
}