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Title: H{sub 2}S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway

Abstract

Hydrogen sulfide (H{sub 2}S), traditionally known for its toxic effects, is now involved in regulating vascular tone. Here we investigated the vasoconstrictive effect of H{sub 2}S on cerebral artery and the underlying mechanism. Sodium hydrosulfide (NaHS), a donor of H{sub 2}S, concentration-dependently induced vasoconstriction on basilar artery, which was enhanced in the presence of isoprenaline, a β-adrenoceptor agonist or forskolin, an adenylyl cyclase activator. Administration of NaHS attenuated the vasorelaxant effects of isoprenaline or forskolin. Meanwhile, the NaHS-induced vasoconstriction was diminished in the presence of 8B-cAMP, an analog of cAMP, but was not affected by Bay K-8644, a selective L-type Ca{sup 2+} channel agonist. These results could be explained by the revised effects of NaHS on isoprenaline-induced cAMP elevation and forskolin-stimulated adenylyl cyclase activity. Additionally, NaHS-induced vasoconstriction was enhanced by removing the endothelium or in the presence of L-NAME, an inhibitor of nitric oxide synthase. L-NAME only partially attenuated the effect of NaHS which was given together with forskolin on the pre-contracted artery. In conclusion, H{sub 2}S induces vasoconstriction of cerebral artery via, at least in part, cAMP/adenylyl cyclase pathway. - Highlights: • The vasoactivity effect of NaHS, a donor of H{sub 2}S, was studied on rat cerebral arteries. •more » H{sub 2}S induces a constriction, not a relaxant effect on basilar arteries. • The vasoconstrictive effect is invovled in inhibiting adenylyl cyclase to reduce cAMP levels. • The vasoconstriction is partially antagonized by NO, and does not necessarily act via NO pathway.« less

Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
22687844
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 289; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMP; ARGININE; CEREBRAL ARTERIES; CONCENTRATION RATIO; CYCLASES; DMSO; ECOLOGICAL CONCENTRATION; ENDOTHELIUM; ESTERS; HYDROGEN SULFIDES; NITRIC OXIDE; PIPERAZINES; RATS; SODIUM HYDRIDES; VASOCONSTRICTION

Citation Formats

Li, Sen, Ping, Na-na, Cao, Lei, E-mail: leicao@mail.xjtu.edu.cn, Mi, Yan-ni, and Cao, Yong-xiao, E-mail: yxy@xjtu.edu.cn. H{sub 2}S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway. United States: N. p., 2015. Web. doi:10.1016/J.TAAP.2015.10.021.
Li, Sen, Ping, Na-na, Cao, Lei, E-mail: leicao@mail.xjtu.edu.cn, Mi, Yan-ni, & Cao, Yong-xiao, E-mail: yxy@xjtu.edu.cn. H{sub 2}S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway. United States. doi:10.1016/J.TAAP.2015.10.021.
Li, Sen, Ping, Na-na, Cao, Lei, E-mail: leicao@mail.xjtu.edu.cn, Mi, Yan-ni, and Cao, Yong-xiao, E-mail: yxy@xjtu.edu.cn. Tue . "H{sub 2}S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway". United States. doi:10.1016/J.TAAP.2015.10.021.
@article{osti_22687844,
title = {H{sub 2}S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway},
author = {Li, Sen and Ping, Na-na and Cao, Lei, E-mail: leicao@mail.xjtu.edu.cn and Mi, Yan-ni and Cao, Yong-xiao, E-mail: yxy@xjtu.edu.cn},
abstractNote = {Hydrogen sulfide (H{sub 2}S), traditionally known for its toxic effects, is now involved in regulating vascular tone. Here we investigated the vasoconstrictive effect of H{sub 2}S on cerebral artery and the underlying mechanism. Sodium hydrosulfide (NaHS), a donor of H{sub 2}S, concentration-dependently induced vasoconstriction on basilar artery, which was enhanced in the presence of isoprenaline, a β-adrenoceptor agonist or forskolin, an adenylyl cyclase activator. Administration of NaHS attenuated the vasorelaxant effects of isoprenaline or forskolin. Meanwhile, the NaHS-induced vasoconstriction was diminished in the presence of 8B-cAMP, an analog of cAMP, but was not affected by Bay K-8644, a selective L-type Ca{sup 2+} channel agonist. These results could be explained by the revised effects of NaHS on isoprenaline-induced cAMP elevation and forskolin-stimulated adenylyl cyclase activity. Additionally, NaHS-induced vasoconstriction was enhanced by removing the endothelium or in the presence of L-NAME, an inhibitor of nitric oxide synthase. L-NAME only partially attenuated the effect of NaHS which was given together with forskolin on the pre-contracted artery. In conclusion, H{sub 2}S induces vasoconstriction of cerebral artery via, at least in part, cAMP/adenylyl cyclase pathway. - Highlights: • The vasoactivity effect of NaHS, a donor of H{sub 2}S, was studied on rat cerebral arteries. • H{sub 2}S induces a constriction, not a relaxant effect on basilar arteries. • The vasoconstrictive effect is invovled in inhibiting adenylyl cyclase to reduce cAMP levels. • The vasoconstriction is partially antagonized by NO, and does not necessarily act via NO pathway.},
doi = {10.1016/J.TAAP.2015.10.021},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 289,
place = {United States},
year = {2015},
month = {12}
}