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Title: 2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation

Abstract

Cadmium is a toxic heavy metal present in the environment and in industrial materials. Cadmium has demonstrated carcinogenic activity that induces cell transformation, but how this occurs is unclear. We used 2D-DIGE and MALDI TOF/TOF MS combined with bioinformatics and immunoblotting to investigate the molecular mechanism of cadmium transformation. We found that small GTPases were critical for transformation. Additionally, proteins involved in mitochondrial transcription, DNA repair, and translation also had altered expression patterns in cadmium treated cells. Collectively, our results suggest that activation of small GTPases contributes to cadmium-induced transformation of colon cells. - Highlights: • Colon epithelial cell line is firstly successfully transformed by cadmium. • 2D-DIGE is applied to visualize the differentially expressed proteins. • RhoA plays an important role in cadmium induced malignant transformation. • Bioinformatic and experimental methods are combined to explore new mechanisms.

Authors:
 [1];  [2];  [1];  [2];  [3];  [1];  [2];  [1];  [4];  [5];  [5]
  1. School of Medicine, Jiangsu University, Zhenjiang 212013 (China)
  2. (China)
  3. Department of Respiration Medicine, Changzhou No.2 People's Hospital, Changzhou 213003 (China)
  4. School of Pharmacy, Jiangsu University, Zhenjiang 212013 (China)
  5. Institute of Life Sciences, Jiangsu University, Zhenjiang 212013 (China)
Publication Date:
OSTI Identifier:
22687771
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 288; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CADMIUM; CARCINOGENS; CELL TRANSFORMATIONS; DNA; DNA REPAIR; HEAVY METALS; LARGE INTESTINE; MITOCHONDRIA; PROTEINS; TOXICITY; TRANSCRIPTION

Citation Formats

Lu, Jian, E-mail: lujian@ujs.edu.cn, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zhou, Zhongping, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zheng, Jianzhou, Zhang, Zhuyi, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Lu, Rongzhu, Liu, Hanqing, Shi, Haifeng, and Tu, Zhigang, E-mail: tuzg_ujs@ujs.edu.cn. 2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation. United States: N. p., 2015. Web. doi:10.1016/J.TAAP.2015.07.020.
Lu, Jian, E-mail: lujian@ujs.edu.cn, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zhou, Zhongping, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zheng, Jianzhou, Zhang, Zhuyi, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Lu, Rongzhu, Liu, Hanqing, Shi, Haifeng, & Tu, Zhigang, E-mail: tuzg_ujs@ujs.edu.cn. 2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation. United States. doi:10.1016/J.TAAP.2015.07.020.
Lu, Jian, E-mail: lujian@ujs.edu.cn, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zhou, Zhongping, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Zheng, Jianzhou, Zhang, Zhuyi, Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, Lu, Rongzhu, Liu, Hanqing, Shi, Haifeng, and Tu, Zhigang, E-mail: tuzg_ujs@ujs.edu.cn. Thu . "2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation". United States. doi:10.1016/J.TAAP.2015.07.020.
@article{osti_22687771,
title = {2D-DIGE and MALDI TOF/TOF MS analysis reveal that small GTPase signaling pathways may play an important role in cadmium-induced colon cell malignant transformation},
author = {Lu, Jian, E-mail: lujian@ujs.edu.cn and Institute of Life Sciences, Jiangsu University, Zhenjiang 212013 and Zhou, Zhongping and Institute of Life Sciences, Jiangsu University, Zhenjiang 212013 and Zheng, Jianzhou and Zhang, Zhuyi and Institute of Life Sciences, Jiangsu University, Zhenjiang 212013 and Lu, Rongzhu and Liu, Hanqing and Shi, Haifeng and Tu, Zhigang, E-mail: tuzg_ujs@ujs.edu.cn},
abstractNote = {Cadmium is a toxic heavy metal present in the environment and in industrial materials. Cadmium has demonstrated carcinogenic activity that induces cell transformation, but how this occurs is unclear. We used 2D-DIGE and MALDI TOF/TOF MS combined with bioinformatics and immunoblotting to investigate the molecular mechanism of cadmium transformation. We found that small GTPases were critical for transformation. Additionally, proteins involved in mitochondrial transcription, DNA repair, and translation also had altered expression patterns in cadmium treated cells. Collectively, our results suggest that activation of small GTPases contributes to cadmium-induced transformation of colon cells. - Highlights: • Colon epithelial cell line is firstly successfully transformed by cadmium. • 2D-DIGE is applied to visualize the differentially expressed proteins. • RhoA plays an important role in cadmium induced malignant transformation. • Bioinformatic and experimental methods are combined to explore new mechanisms.},
doi = {10.1016/J.TAAP.2015.07.020},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 288,
place = {United States},
year = {2015},
month = {10}
}