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Title: Effects of amorphous silica coating on cerium oxide nanoparticles induced pulmonary responses

Abstract

Recently cerium compounds have been used in a variety of consumer products, including diesel fuel additives, to increase fuel combustion efficiency and decrease diesel soot emissions. However, cerium oxide (CeO{sub 2}) nanoparticles have been detected in the exhaust, which raises a health concern. Previous studies have shown that exposure of rats to nanoscale CeO{sub 2} by intratracheal instillation (IT) induces sustained pulmonary inflammation and fibrosis. In the present study, male Sprague–Dawley rats were exposed to CeO{sub 2} or CeO{sub 2} coated with a nano layer of amorphous SiO{sub 2} (aSiO{sub 2}/CeO{sub 2}) by a single IT and sacrificed at various times post-exposure to assess potential protective effects of the aSiO{sub 2} coating. The first acellular bronchoalveolar lavage (BAL) fluid and BAL cells were collected and analyzed from all exposed animals. At the low dose (0.15 mg/kg), CeO{sub 2} but not aSiO{sub 2}/CeO{sub 2} exposure induced inflammation. However, at the higher doses, both particles induced a dose-related inflammation, cytotoxicity, inflammatory cytokines, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP at 1 day post-exposure. Morphological analysis of lung showed an increased inflammation, surfactant and collagen fibers after CeO{sub 2} (high dose at 3.5 mg/kg) treatment at 28 days post-exposure. aSiO{sub 2} coatingmore » significantly reduced CeO{sub 2}-induced inflammatory responses in the airspace and appeared to attenuate phospholipidosis and fibrosis. Energy dispersive X-ray spectroscopy analysis showed Ce and phosphorous (P) in all particle-exposed lungs, whereas Si was only detected in aSiO{sub 2}/CeO{sub 2}-exposed lungs up to 3 days after exposure, suggesting that aSiO{sub 2} dissolved off the CeO{sub 2} core, and some of the CeO{sub 2} was transformed to CePO{sub 4} with time. These results demonstrate that aSiO{sub 2} coating reduce CeO{sub 2}-induced inflammation, phospholipidosis and fibrosis. - Highlights: • Both CeO{sub 2} and aSiO{sub 2}/CeO{sub 2} particles were detected in the respective particle-exposed lungs. • The dissolution of aSiO{sub 2} coating from CeO{sub 2} particle core with time was demonstrated in the particle-exposed lungs. • aSiO{sub 2} coating significantly protected CeO{sub 2}-induced pulmonary inflammatory responses. • aSiO{sub 2} coating showed a protective effect on CeO{sub 2}-induced lung fibrosis.« less

Authors:
 [1]; ; ;  [1]; ;  [2];  [3]
  1. Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV (United States)
  2. Harvard TH Chan School of Public Health, Harvard University, Boston, MA (United States)
  3. School of Pharmacy, West Virginia University, Morgantown, WV (United States)
Publication Date:
OSTI Identifier:
22687767
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 288; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CERIUM; CERIUM OXIDES; CERIUM PHOSPHATES; COLLAGEN; COMBUSTION; CONSUMER PRODUCTS; DIESEL FUELS; FIBROSIS; FUEL ADDITIVES; INFLAMMATION; LUNGS; LYMPHOKINES; NANOPARTICLES; RATS; SILICA; SILICON OXIDES; SOOT; X-RAY SPECTROSCOPY

Citation Formats

Ma, Jane, E-mail: jym1@cdc.gov, Mercer, Robert R., Barger, Mark, Schwegler-Berry, Diane, Cohen, Joel M., Demokritou, Philip, and Castranova, Vincent. Effects of amorphous silica coating on cerium oxide nanoparticles induced pulmonary responses. United States: N. p., 2015. Web. doi:10.1016/J.TAAP.2015.07.012.
Ma, Jane, E-mail: jym1@cdc.gov, Mercer, Robert R., Barger, Mark, Schwegler-Berry, Diane, Cohen, Joel M., Demokritou, Philip, & Castranova, Vincent. Effects of amorphous silica coating on cerium oxide nanoparticles induced pulmonary responses. United States. doi:10.1016/J.TAAP.2015.07.012.
Ma, Jane, E-mail: jym1@cdc.gov, Mercer, Robert R., Barger, Mark, Schwegler-Berry, Diane, Cohen, Joel M., Demokritou, Philip, and Castranova, Vincent. Thu . "Effects of amorphous silica coating on cerium oxide nanoparticles induced pulmonary responses". United States. doi:10.1016/J.TAAP.2015.07.012.
@article{osti_22687767,
title = {Effects of amorphous silica coating on cerium oxide nanoparticles induced pulmonary responses},
author = {Ma, Jane, E-mail: jym1@cdc.gov and Mercer, Robert R. and Barger, Mark and Schwegler-Berry, Diane and Cohen, Joel M. and Demokritou, Philip and Castranova, Vincent},
abstractNote = {Recently cerium compounds have been used in a variety of consumer products, including diesel fuel additives, to increase fuel combustion efficiency and decrease diesel soot emissions. However, cerium oxide (CeO{sub 2}) nanoparticles have been detected in the exhaust, which raises a health concern. Previous studies have shown that exposure of rats to nanoscale CeO{sub 2} by intratracheal instillation (IT) induces sustained pulmonary inflammation and fibrosis. In the present study, male Sprague–Dawley rats were exposed to CeO{sub 2} or CeO{sub 2} coated with a nano layer of amorphous SiO{sub 2} (aSiO{sub 2}/CeO{sub 2}) by a single IT and sacrificed at various times post-exposure to assess potential protective effects of the aSiO{sub 2} coating. The first acellular bronchoalveolar lavage (BAL) fluid and BAL cells were collected and analyzed from all exposed animals. At the low dose (0.15 mg/kg), CeO{sub 2} but not aSiO{sub 2}/CeO{sub 2} exposure induced inflammation. However, at the higher doses, both particles induced a dose-related inflammation, cytotoxicity, inflammatory cytokines, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP at 1 day post-exposure. Morphological analysis of lung showed an increased inflammation, surfactant and collagen fibers after CeO{sub 2} (high dose at 3.5 mg/kg) treatment at 28 days post-exposure. aSiO{sub 2} coating significantly reduced CeO{sub 2}-induced inflammatory responses in the airspace and appeared to attenuate phospholipidosis and fibrosis. Energy dispersive X-ray spectroscopy analysis showed Ce and phosphorous (P) in all particle-exposed lungs, whereas Si was only detected in aSiO{sub 2}/CeO{sub 2}-exposed lungs up to 3 days after exposure, suggesting that aSiO{sub 2} dissolved off the CeO{sub 2} core, and some of the CeO{sub 2} was transformed to CePO{sub 4} with time. These results demonstrate that aSiO{sub 2} coating reduce CeO{sub 2}-induced inflammation, phospholipidosis and fibrosis. - Highlights: • Both CeO{sub 2} and aSiO{sub 2}/CeO{sub 2} particles were detected in the respective particle-exposed lungs. • The dissolution of aSiO{sub 2} coating from CeO{sub 2} particle core with time was demonstrated in the particle-exposed lungs. • aSiO{sub 2} coating significantly protected CeO{sub 2}-induced pulmonary inflammatory responses. • aSiO{sub 2} coating showed a protective effect on CeO{sub 2}-induced lung fibrosis.},
doi = {10.1016/J.TAAP.2015.07.012},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 288,
place = {United States},
year = {2015},
month = {10}
}