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Title: Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles

Abstract

The aim of this study was to investigate the inflammatory and immunological responses in airways and lung-draining lymph nodes (LDLNs), following lung exposure to iron oxide (hematite) nanoparticles (NPs). The responses to the hematite NPs were evaluated in both healthy non-sensitized mice, and in sensitized mice with an established allergic airway disease. The mice were exposed intratracheally to either hematite NPs or to vehicle (PBS) and the cellular responses were evaluated on days 1, 2, and 7, post-exposure. Exposure to hematite NPs increased the numbers of neutrophils, eosinophils, and lymphocytes in the airways of non-sensitized mice on days 1 and 2 post-exposure; at these time points the number of lymphocytes was also elevated in the LDLNs. In contrast, exposing sensitized mice to hematite NPs induced a rapid and unspecific cellular reduction in the alveolar space on day 1 post-exposure; a similar decrease of lymphocytes was also observed in the LDLN. The results indicate that cells in the airways and in the LDLN of individuals with established airway inflammation undergo cell death when exposed to hematite NPs. A possible explanation for this toxic response is the extensive generation of reactive oxygen species (ROS) in the pro-oxidative environment of inflamed airways. Thismore » study demonstrates how sensitized and non-sensitized mice respond differently to hematite NP exposure, and it highlights the importance of including individuals with respiratory disorders when evaluating health effects of inhaled nanomaterials. - Highlights: • Hematite NPs induce differential responses in airways of healthy and allergic mice. • Hematite induced an airway inflammation in healthy mice. • Hematite induced cellular reduction in the alveolus and lymph nodes of allergic mice. • Cell death is possible due to extensive pro-oxidative environment in allergic mice. • It is important to include sensitive individuals when valuing health effects of NPs.« less

Authors:
 [1];  [2];  [1];  [2];  [1];  [3];  [4];  [1];  [2]
  1. Swedish Defence Research Agency, Division of CBRN Defence and Security, Umeå (Sweden)
  2. (Sweden)
  3. Dept of Engineering Sciences, The Ångström Laboratory, Uppsala University, SE-751 Uppsala (Sweden)
  4. Dept of Public Health and Clinical Medicine, Umeå University (Sweden)
Publication Date:
OSTI Identifier:
22687760
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 288; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; EOSINOPHILS; FERRITES; HEMATITE; INFLAMMATION; IRON; IRON OXIDES; LUNGS; LYMPH; LYMPH NODES; LYMPHOCYTES; MICE; NANOPARTICLES; NEUTROPHILS; OVALBUMIN; OXIDATION; OXYGEN; TOXICITY

Citation Formats

Gustafsson, Åsa, E-mail: asa.gustafsson@foi.se, Dept of Public Health and Clinical Medicine, Umeå University, Bergström, Ulrika, Dept of Organismal Biology, Uppsala University, SE-751 Uppsala, Ågren, Lina, Österlund, Lars, Sandström, Thomas, Bucht, Anders, and Dept of Public Health and Clinical Medicine, Umeå University. Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles. United States: N. p., 2015. Web. doi:10.1016/J.TAAP.2015.07.001.
Gustafsson, Åsa, E-mail: asa.gustafsson@foi.se, Dept of Public Health and Clinical Medicine, Umeå University, Bergström, Ulrika, Dept of Organismal Biology, Uppsala University, SE-751 Uppsala, Ågren, Lina, Österlund, Lars, Sandström, Thomas, Bucht, Anders, & Dept of Public Health and Clinical Medicine, Umeå University. Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles. United States. doi:10.1016/J.TAAP.2015.07.001.
Gustafsson, Åsa, E-mail: asa.gustafsson@foi.se, Dept of Public Health and Clinical Medicine, Umeå University, Bergström, Ulrika, Dept of Organismal Biology, Uppsala University, SE-751 Uppsala, Ågren, Lina, Österlund, Lars, Sandström, Thomas, Bucht, Anders, and Dept of Public Health and Clinical Medicine, Umeå University. Thu . "Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles". United States. doi:10.1016/J.TAAP.2015.07.001.
@article{osti_22687760,
title = {Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles},
author = {Gustafsson, Åsa, E-mail: asa.gustafsson@foi.se and Dept of Public Health and Clinical Medicine, Umeå University and Bergström, Ulrika and Dept of Organismal Biology, Uppsala University, SE-751 Uppsala and Ågren, Lina and Österlund, Lars and Sandström, Thomas and Bucht, Anders and Dept of Public Health and Clinical Medicine, Umeå University},
abstractNote = {The aim of this study was to investigate the inflammatory and immunological responses in airways and lung-draining lymph nodes (LDLNs), following lung exposure to iron oxide (hematite) nanoparticles (NPs). The responses to the hematite NPs were evaluated in both healthy non-sensitized mice, and in sensitized mice with an established allergic airway disease. The mice were exposed intratracheally to either hematite NPs or to vehicle (PBS) and the cellular responses were evaluated on days 1, 2, and 7, post-exposure. Exposure to hematite NPs increased the numbers of neutrophils, eosinophils, and lymphocytes in the airways of non-sensitized mice on days 1 and 2 post-exposure; at these time points the number of lymphocytes was also elevated in the LDLNs. In contrast, exposing sensitized mice to hematite NPs induced a rapid and unspecific cellular reduction in the alveolar space on day 1 post-exposure; a similar decrease of lymphocytes was also observed in the LDLN. The results indicate that cells in the airways and in the LDLN of individuals with established airway inflammation undergo cell death when exposed to hematite NPs. A possible explanation for this toxic response is the extensive generation of reactive oxygen species (ROS) in the pro-oxidative environment of inflamed airways. This study demonstrates how sensitized and non-sensitized mice respond differently to hematite NP exposure, and it highlights the importance of including individuals with respiratory disorders when evaluating health effects of inhaled nanomaterials. - Highlights: • Hematite NPs induce differential responses in airways of healthy and allergic mice. • Hematite induced an airway inflammation in healthy mice. • Hematite induced cellular reduction in the alveolus and lymph nodes of allergic mice. • Cell death is possible due to extensive pro-oxidative environment in allergic mice. • It is important to include sensitive individuals when valuing health effects of NPs.},
doi = {10.1016/J.TAAP.2015.07.001},
journal = {Toxicology and Applied Pharmacology},
number = 1,
volume = 288,
place = {United States},
year = {Thu Oct 01 00:00:00 EDT 2015},
month = {Thu Oct 01 00:00:00 EDT 2015}
}