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Title: Characterization of stem-like cells in a new astroblastoma cell line

Abstract

Cell lines established from tumors are the most commonly used models in cancer research, and their use in recent years has enabled a greater understanding of the biology of cancer and the means to develop effective treatment strategies. Astroblastomas are uncommon neuroepithelial tumors of glial origin, predominantly affecting young people, mainly teenagers and children, predominantly females. To date, only a single study has reported that astroblastomas contain a large number of neural stem-like cells, which had only a partial proliferation capacity and differentiation. Our objective was to establish an astroblastoma cell line to investigate the presence of astroblastic cells and cancer stem-like cells. The migratory and invasion abilities of the cells were quantified with invasion and migration assays and compared to a glioblastoma cell line. The presence of stem cells was detected with surface-marker analysis by using flow cytometry, and measuring the differentiation ability with a differentiation assay and the self-renewal capacity with a sphere-forming assay. These characteristics may determine whether this novel cell line is a model for astroblastomas that may have stem-cell characteristics. With this novel cell line, scientists can investigate the molecular pathways underlying astroblastomas and develop new therapeutic strategies for patients with these tumors. - Highlights:more » • An establishment of a novel astroblastoma cell line was proposed. • The presence of astroblastic cells and cancer stem-like cells was investigated. • The molecular pathways underlying astroblastomas may be investigated. • New therapeutic strategies for patients with astroblastoma may be developed.« less

Authors:
;  [1];  [2]; ;  [3];  [1];  [4];  [1];  [3]
  1. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul (Turkey)
  2. Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum (Turkey)
  3. Department of Medical Genetics, Yeditepe University Medical School and Yeditepe University Hospital, Istanbul (Turkey)
  4. Department of Neurosurgery, Yeditepe University School of Medicine, Istanbul (Turkey)
Publication Date:
OSTI Identifier:
22649844
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 352; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGY; CELL PROLIFERATION; CHILDREN; FEMALES; GLIOMAS; MIGRATION; PATIENTS; STEM CELLS

Citation Formats

Coban, Esra Aydemir, Kasikci, Ezgi, Karatas, Omer Faruk, Suakar, Oznur, Kuskucu, Aysegul, Altunbek, Mine, Türe, Uğur, Sahin, Fikrettin, and Bayrak, Omer Faruk, E-mail: ofbayrak@yeditepe.edu.tr. Characterization of stem-like cells in a new astroblastoma cell line. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2017.02.030.
Coban, Esra Aydemir, Kasikci, Ezgi, Karatas, Omer Faruk, Suakar, Oznur, Kuskucu, Aysegul, Altunbek, Mine, Türe, Uğur, Sahin, Fikrettin, & Bayrak, Omer Faruk, E-mail: ofbayrak@yeditepe.edu.tr. Characterization of stem-like cells in a new astroblastoma cell line. United States. doi:10.1016/J.YEXCR.2017.02.030.
Coban, Esra Aydemir, Kasikci, Ezgi, Karatas, Omer Faruk, Suakar, Oznur, Kuskucu, Aysegul, Altunbek, Mine, Türe, Uğur, Sahin, Fikrettin, and Bayrak, Omer Faruk, E-mail: ofbayrak@yeditepe.edu.tr. Wed . "Characterization of stem-like cells in a new astroblastoma cell line". United States. doi:10.1016/J.YEXCR.2017.02.030.
@article{osti_22649844,
title = {Characterization of stem-like cells in a new astroblastoma cell line},
author = {Coban, Esra Aydemir and Kasikci, Ezgi and Karatas, Omer Faruk and Suakar, Oznur and Kuskucu, Aysegul and Altunbek, Mine and Türe, Uğur and Sahin, Fikrettin and Bayrak, Omer Faruk, E-mail: ofbayrak@yeditepe.edu.tr},
abstractNote = {Cell lines established from tumors are the most commonly used models in cancer research, and their use in recent years has enabled a greater understanding of the biology of cancer and the means to develop effective treatment strategies. Astroblastomas are uncommon neuroepithelial tumors of glial origin, predominantly affecting young people, mainly teenagers and children, predominantly females. To date, only a single study has reported that astroblastomas contain a large number of neural stem-like cells, which had only a partial proliferation capacity and differentiation. Our objective was to establish an astroblastoma cell line to investigate the presence of astroblastic cells and cancer stem-like cells. The migratory and invasion abilities of the cells were quantified with invasion and migration assays and compared to a glioblastoma cell line. The presence of stem cells was detected with surface-marker analysis by using flow cytometry, and measuring the differentiation ability with a differentiation assay and the self-renewal capacity with a sphere-forming assay. These characteristics may determine whether this novel cell line is a model for astroblastomas that may have stem-cell characteristics. With this novel cell line, scientists can investigate the molecular pathways underlying astroblastomas and develop new therapeutic strategies for patients with these tumors. - Highlights: • An establishment of a novel astroblastoma cell line was proposed. • The presence of astroblastic cells and cancer stem-like cells was investigated. • The molecular pathways underlying astroblastomas may be investigated. • New therapeutic strategies for patients with astroblastoma may be developed.},
doi = {10.1016/J.YEXCR.2017.02.030},
journal = {Experimental Cell Research},
number = 2,
volume = 352,
place = {United States},
year = {Wed Mar 15 00:00:00 EDT 2017},
month = {Wed Mar 15 00:00:00 EDT 2017}
}