skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells

Abstract

Lung cancer is the common cause of death from cancer, and most lung cancer patients die of metastasis. MicroRNAs (miRNAs) function as either oncogenes or tumor suppressors, playing crucial role not only in tumorigenesis, but also in tumor invasion and metastasis. There are several studies showed that miR-26a is involved in carcinogenesis, however, its role in tumor metastasis need to be elucidated. In this study, we showed that ectopic expression of miR-26a enhanced migration and invasion of lung cancer cells. Glycogen synthase kinase-3β (GSK3β) was identified as a direct target of miR-26a. GSK3β expression negatively correlated with miR-26a expression in lung cancer tissues. Silencing of GSK3β achieved similar effect as miR-26a over-expression; over-expression of GSK3β reversed the enhanced effect of miR-26a on lung cancer cell migration and invasion. Further study indicated that miR-26a increased β-catenin expression and nuclear translocation. C-myc and cyclin D1, the downstream genes of β-catenin, were also up-regulated by miR-26a. Furthermore, xenograft study showed that miR-26a promoted lung cancer cell growth in vivo, and suppressed GSK3β expression. Collectively, our results demonstrated that miR-26a enhanced metastatic potential of lung cancer cells via activation of β-catenin pathway by targeting GSK3β, suggesting the potential applicability of miR-26a as a targetmore » for cancer treatment. - Highlights: • miR-26a enhances migration and invasion of lung cancer cells. • GSK3β is identified as a direct target of miR-26a. • miR-26a activates β-catenin pathway by targeting GSK3β. • miR-26a promotes lung cancer cell growth in vivo.« less

Authors:
;  [1];  [2];  [3];  [1];  [4];  [1];  [1]
  1. Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052 (China)
  2. Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052 (China)
  3. School of Laboratory Medicine, Tianjin Medical University, Tianjin 300052 (China)
  4. Core Facility Center, Tianjin Medical University General Hospital, Tianjin 300052 (China)
Publication Date:
OSTI Identifier:
22649842
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 352; Journal Issue: 2; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CARCINOGENESIS; DEATH; GLYCOGEN; IN VIVO; LUNGS; METASTASES; NEOPLASMS; ONCOGENES; PATIENTS; PHOSPHOTRANSFERASES; TRANSLOCATION

Citation Formats

Lin, Gaoyang, Liu, Boning, Meng, Zhaowei, Liu, Yunde, Li, Xuebing, Wu, Xiang, Zhou, Qinghua, and Xu, Ke, E-mail: ke_xu@hotmail.com. MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2017.02.033.
Lin, Gaoyang, Liu, Boning, Meng, Zhaowei, Liu, Yunde, Li, Xuebing, Wu, Xiang, Zhou, Qinghua, & Xu, Ke, E-mail: ke_xu@hotmail.com. MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells. United States. doi:10.1016/J.YEXCR.2017.02.033.
Lin, Gaoyang, Liu, Boning, Meng, Zhaowei, Liu, Yunde, Li, Xuebing, Wu, Xiang, Zhou, Qinghua, and Xu, Ke, E-mail: ke_xu@hotmail.com. Wed . "MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells". United States. doi:10.1016/J.YEXCR.2017.02.033.
@article{osti_22649842,
title = {MiR-26a enhances invasive capacity by suppressing GSK3β in human lung cancer cells},
author = {Lin, Gaoyang and Liu, Boning and Meng, Zhaowei and Liu, Yunde and Li, Xuebing and Wu, Xiang and Zhou, Qinghua and Xu, Ke, E-mail: ke_xu@hotmail.com},
abstractNote = {Lung cancer is the common cause of death from cancer, and most lung cancer patients die of metastasis. MicroRNAs (miRNAs) function as either oncogenes or tumor suppressors, playing crucial role not only in tumorigenesis, but also in tumor invasion and metastasis. There are several studies showed that miR-26a is involved in carcinogenesis, however, its role in tumor metastasis need to be elucidated. In this study, we showed that ectopic expression of miR-26a enhanced migration and invasion of lung cancer cells. Glycogen synthase kinase-3β (GSK3β) was identified as a direct target of miR-26a. GSK3β expression negatively correlated with miR-26a expression in lung cancer tissues. Silencing of GSK3β achieved similar effect as miR-26a over-expression; over-expression of GSK3β reversed the enhanced effect of miR-26a on lung cancer cell migration and invasion. Further study indicated that miR-26a increased β-catenin expression and nuclear translocation. C-myc and cyclin D1, the downstream genes of β-catenin, were also up-regulated by miR-26a. Furthermore, xenograft study showed that miR-26a promoted lung cancer cell growth in vivo, and suppressed GSK3β expression. Collectively, our results demonstrated that miR-26a enhanced metastatic potential of lung cancer cells via activation of β-catenin pathway by targeting GSK3β, suggesting the potential applicability of miR-26a as a target for cancer treatment. - Highlights: • miR-26a enhances migration and invasion of lung cancer cells. • GSK3β is identified as a direct target of miR-26a. • miR-26a activates β-catenin pathway by targeting GSK3β. • miR-26a promotes lung cancer cell growth in vivo.},
doi = {10.1016/J.YEXCR.2017.02.033},
journal = {Experimental Cell Research},
number = 2,
volume = 352,
place = {United States},
year = {Wed Mar 15 00:00:00 EDT 2017},
month = {Wed Mar 15 00:00:00 EDT 2017}
}