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Title: Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage

Abstract

Objectives: Autophagy constitutes a defense mechanism to overcome aging and apoptosis in osteoarthritic cartilage. Several cytokines and transcription factors are linked to autophagy and play an important role in the degradative cascade in osteoarthritis (OA). Cell therapy such as platelet rich plasma (PRP) has recently emerged as a promising therapeutic tool for many diseases including OA. However, its mechanism of action on improving cartilage repair remains to be determined. The purpose of this study is to investigate the effect of PRP on osteoarthritic chondrocytes and to elucidate the mechanism by which PRP contributes to cartilage regeneration. Methods: Osteoarthritic chondrocytes were co-cultured with an increasing concentration of PRP obtained from healthy donors. The effect of PRP on the proliferation of chondrocytes was performed using cell counting and WST8 proliferation assays. Autophagy, apoptosis and intracellular level of IL-4, IL-10, and IL-13 were determined using flow cytometry analyses. Autophagy markers BECLIN and LC3II were also determined using quantitative polymerase chain reaction (qPCR). qPCR and ELISA were used to measure the expression of ADAMDTS-5, MMP3, MMP13, TIMP-1–2–3, aggregan, Collagen type 2, TGF-β, Cox-2, Il-6, FOXO1, FOXO3, and HIF-1 in tissues and co-cultured media. Results: PRP increased significantly the proliferation of chondrocytes, decreased apoptosis andmore » increased autophagy and its markers along with its regulators FOXO1, FOXO3 and HIF-1 in osteoarthritic chondrocytes. Furthermore, PRP caused a dose-dependent significant decrease in MMP3, MMP13, and ADAMTS-5, IL-6 and COX-2 while increasing TGF-β, aggregan, and collagen type 2, TIMPs and intracellular IL-4, IL-10, IL-13. Conclusion: These results suggest that PRP could be a potential therapeutic tool for the treatment of OA. - Highlights: • Platelet Rich Plasma is suggested as a new treatment for osteoarthritis. • The proposed therapeutic effect is chondroprotection. • Chondroprotection is assumed via two major mechanisms:1-by increasing the major players in cartilage protection as as autophagy 2-by decreasing the markers of cartilage degradation such as MMPs.« less

Authors:
 [1];  [2];  [3];  [1];  [4];  [1];  [5];  [1];  [1]
  1. Regenerative medicine and inflammation Laboratory, Faculty of Medicine, Saint-Joseph University, Beirut (Lebanon)
  2. Research Centre in Osteoarthritis, Research Centre in Monteral University (Canada)
  3. Cancer and metabolism Laboratory, Faculty of Medicine, Saint-Joseph University, Beirut (Lebanon)
  4. Hotel Dieu de France, Faculty of Medicine, Saint-Joseph University, Beirut (Lebanon)
  5. Carantina Hospital, Beirut (Lebanon)
Publication Date:
OSTI Identifier:
22649830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 352; Journal Issue: 1; Other Information: Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CARTILAGE; CHAIN REACTIONS; COLLAGEN; CULTURE MEDIA; CYSTEINE; ECOLOGICAL CONCENTRATION; ENZYME IMMUNOASSAY; FIBROBLASTS; INFLAMMATION; INSULIN; LYMPHOKINES; LYMPHOMAS; MICROTUBULES; NECROSIS; PLANT GROWTH; POLYMERASE CHAIN REACTION; POLYMERASES; SAFETY; THERAPY; TRANSCRIPTION; TRANSCRIPTION FACTORS; YEASTS

Citation Formats

Moussa, Mayssam, E-mail: Moussa-mayssam@hotmail.com, Lajeunesse, Daniel, E-mail: daniel.lajeunesse@umontreal.ca, Hilal, George, E-mail: George2266@gmail.com, El Atat, Oula, E-mail: oulaatat@hotmail.com, Haykal, Gaby, E-mail: Gaby.haykal@hdf.usj.edu.lb, Serhal, Rim, E-mail: rim.basbous@gmail.com, Chalhoub, Antonio, E-mail: Mava.o@hotmail.com, Khalil, Charbel, E-mail: charbelk3@hotmail.com, and Alaaeddine, Nada, E-mail: Nada.aladdin@gmail.com. Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2017.02.012.
Moussa, Mayssam, E-mail: Moussa-mayssam@hotmail.com, Lajeunesse, Daniel, E-mail: daniel.lajeunesse@umontreal.ca, Hilal, George, E-mail: George2266@gmail.com, El Atat, Oula, E-mail: oulaatat@hotmail.com, Haykal, Gaby, E-mail: Gaby.haykal@hdf.usj.edu.lb, Serhal, Rim, E-mail: rim.basbous@gmail.com, Chalhoub, Antonio, E-mail: Mava.o@hotmail.com, Khalil, Charbel, E-mail: charbelk3@hotmail.com, & Alaaeddine, Nada, E-mail: Nada.aladdin@gmail.com. Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage. United States. doi:10.1016/J.YEXCR.2017.02.012.
Moussa, Mayssam, E-mail: Moussa-mayssam@hotmail.com, Lajeunesse, Daniel, E-mail: daniel.lajeunesse@umontreal.ca, Hilal, George, E-mail: George2266@gmail.com, El Atat, Oula, E-mail: oulaatat@hotmail.com, Haykal, Gaby, E-mail: Gaby.haykal@hdf.usj.edu.lb, Serhal, Rim, E-mail: rim.basbous@gmail.com, Chalhoub, Antonio, E-mail: Mava.o@hotmail.com, Khalil, Charbel, E-mail: charbelk3@hotmail.com, and Alaaeddine, Nada, E-mail: Nada.aladdin@gmail.com. Wed . "Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage". United States. doi:10.1016/J.YEXCR.2017.02.012.
@article{osti_22649830,
title = {Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage},
author = {Moussa, Mayssam, E-mail: Moussa-mayssam@hotmail.com and Lajeunesse, Daniel, E-mail: daniel.lajeunesse@umontreal.ca and Hilal, George, E-mail: George2266@gmail.com and El Atat, Oula, E-mail: oulaatat@hotmail.com and Haykal, Gaby, E-mail: Gaby.haykal@hdf.usj.edu.lb and Serhal, Rim, E-mail: rim.basbous@gmail.com and Chalhoub, Antonio, E-mail: Mava.o@hotmail.com and Khalil, Charbel, E-mail: charbelk3@hotmail.com and Alaaeddine, Nada, E-mail: Nada.aladdin@gmail.com},
abstractNote = {Objectives: Autophagy constitutes a defense mechanism to overcome aging and apoptosis in osteoarthritic cartilage. Several cytokines and transcription factors are linked to autophagy and play an important role in the degradative cascade in osteoarthritis (OA). Cell therapy such as platelet rich plasma (PRP) has recently emerged as a promising therapeutic tool for many diseases including OA. However, its mechanism of action on improving cartilage repair remains to be determined. The purpose of this study is to investigate the effect of PRP on osteoarthritic chondrocytes and to elucidate the mechanism by which PRP contributes to cartilage regeneration. Methods: Osteoarthritic chondrocytes were co-cultured with an increasing concentration of PRP obtained from healthy donors. The effect of PRP on the proliferation of chondrocytes was performed using cell counting and WST8 proliferation assays. Autophagy, apoptosis and intracellular level of IL-4, IL-10, and IL-13 were determined using flow cytometry analyses. Autophagy markers BECLIN and LC3II were also determined using quantitative polymerase chain reaction (qPCR). qPCR and ELISA were used to measure the expression of ADAMDTS-5, MMP3, MMP13, TIMP-1–2–3, aggregan, Collagen type 2, TGF-β, Cox-2, Il-6, FOXO1, FOXO3, and HIF-1 in tissues and co-cultured media. Results: PRP increased significantly the proliferation of chondrocytes, decreased apoptosis and increased autophagy and its markers along with its regulators FOXO1, FOXO3 and HIF-1 in osteoarthritic chondrocytes. Furthermore, PRP caused a dose-dependent significant decrease in MMP3, MMP13, and ADAMTS-5, IL-6 and COX-2 while increasing TGF-β, aggregan, and collagen type 2, TIMPs and intracellular IL-4, IL-10, IL-13. Conclusion: These results suggest that PRP could be a potential therapeutic tool for the treatment of OA. - Highlights: • Platelet Rich Plasma is suggested as a new treatment for osteoarthritis. • The proposed therapeutic effect is chondroprotection. • Chondroprotection is assumed via two major mechanisms:1-by increasing the major players in cartilage protection as as autophagy 2-by decreasing the markers of cartilage degradation such as MMPs.},
doi = {10.1016/J.YEXCR.2017.02.012},
journal = {Experimental Cell Research},
number = 1,
volume = 352,
place = {United States},
year = {Wed Mar 01 00:00:00 EST 2017},
month = {Wed Mar 01 00:00:00 EST 2017}
}
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