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Title: STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells

Abstract

NADPH oxidase (NOX) generates reactive oxygen species (ROS) and has been suggested to mediate cell proliferation in some cancers. Here, we show that an increase in the expression of NOX5 long form (NOX5-L) is critical for tumor progression in breast tumor tissues. Immunostaining of clinical samples indicated that NOX5 was overexpressed in 41.1% of breast ductal carcinoma samples. NOX5-L depletion consistently suppressed cell proliferation, invasion, and migration in vitro. Antibody-mediated neutralization of NOX5-L attenuated tumor progression in a mouse xenograft model. Promoter analysis revealed that NOX5-L expression is regulated by STAT5A in breast cancer cells. Based on our novel findings, we suggest that inhibition of NOX5-L may be a promising therapeutic strategy that exerts anti-cancer effects via the modulation of ROS-mediated cell signaling. - Highlights: • The ROS-generating protein, NOX5-L, determines cellular proliferation and metastasis in subset of breast tumor. • Tumor growth was attenuated by the treatment of anti-NOX5-L antibody in a xenograft model. • NOX5-L expression is transcriptionally regulated by STAT5A in breast cancer cells.

Authors:
 [1];  [2]; ; ;  [1];  [3];  [4];  [4];  [1];  [5]
  1. Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806 (Korea, Republic of)
  2. (Korea, Republic of)
  3. Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353 (Korea, Republic of)
  4. Department of Pathology, Soonchunhyang Medical Science Research Institute, Chonan 330-090 (Korea, Republic of)
  5. (UST), Daejeon 305-333 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22649820
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 351; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ANTIBODIES; CARCINOMAS; CELL PROLIFERATION; IN VITRO; INHIBITION; MAMMARY GLANDS; METASTASES; MICE; MODULATION; OXIDASES; OXYGEN; PLANT GROWTH; PROMOTERS; SIGNALS

Citation Formats

Dho, So Hee, Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353, Kim, Ji Young, Lee, Kwang-Pyo, Kwon, Eun-Soo, Lim, Jae Cheong, Kim, Chang-Jin, Jeong, Dongjun, E-mail: juny1024@sch.ac.kr, Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr, and Department of Functional Genomics, Korea University of Science and Technology. STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2016.12.020.
Dho, So Hee, Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353, Kim, Ji Young, Lee, Kwang-Pyo, Kwon, Eun-Soo, Lim, Jae Cheong, Kim, Chang-Jin, Jeong, Dongjun, E-mail: juny1024@sch.ac.kr, Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr, & Department of Functional Genomics, Korea University of Science and Technology. STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells. United States. doi:10.1016/J.YEXCR.2016.12.020.
Dho, So Hee, Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353, Kim, Ji Young, Lee, Kwang-Pyo, Kwon, Eun-Soo, Lim, Jae Cheong, Kim, Chang-Jin, Jeong, Dongjun, E-mail: juny1024@sch.ac.kr, Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr, and Department of Functional Genomics, Korea University of Science and Technology. Wed . "STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells". United States. doi:10.1016/J.YEXCR.2016.12.020.
@article{osti_22649820,
title = {STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells},
author = {Dho, So Hee and Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute, Daejeon 305-353 and Kim, Ji Young and Lee, Kwang-Pyo and Kwon, Eun-Soo and Lim, Jae Cheong and Kim, Chang-Jin and Jeong, Dongjun, E-mail: juny1024@sch.ac.kr and Kwon, Ki-Sun, E-mail: kwonks@kribb.re.kr and Department of Functional Genomics, Korea University of Science and Technology},
abstractNote = {NADPH oxidase (NOX) generates reactive oxygen species (ROS) and has been suggested to mediate cell proliferation in some cancers. Here, we show that an increase in the expression of NOX5 long form (NOX5-L) is critical for tumor progression in breast tumor tissues. Immunostaining of clinical samples indicated that NOX5 was overexpressed in 41.1% of breast ductal carcinoma samples. NOX5-L depletion consistently suppressed cell proliferation, invasion, and migration in vitro. Antibody-mediated neutralization of NOX5-L attenuated tumor progression in a mouse xenograft model. Promoter analysis revealed that NOX5-L expression is regulated by STAT5A in breast cancer cells. Based on our novel findings, we suggest that inhibition of NOX5-L may be a promising therapeutic strategy that exerts anti-cancer effects via the modulation of ROS-mediated cell signaling. - Highlights: • The ROS-generating protein, NOX5-L, determines cellular proliferation and metastasis in subset of breast tumor. • Tumor growth was attenuated by the treatment of anti-NOX5-L antibody in a xenograft model. • NOX5-L expression is transcriptionally regulated by STAT5A in breast cancer cells.},
doi = {10.1016/J.YEXCR.2016.12.020},
journal = {Experimental Cell Research},
number = 1,
volume = 351,
place = {United States},
year = {Wed Feb 01 00:00:00 EST 2017},
month = {Wed Feb 01 00:00:00 EST 2017}
}