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Title: MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro

Abstract

Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.

Authors:
 [1];  [2];  [3];  [1];  [4];  [5];  [1];  [6];  [7]; ; ;  [8];  [6];
  1. Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu (Finland)
  2. Department of Pathology, University of Turku, Turku University Hospital, Turku (Finland)
  3. (Finland)
  4. Department of Pathology, Haartman Institute, University of Helsinki, Helsinki (Finland)
  5. Department of Pathology, HUSLAB, Helsinki (Finland)
  6. Department of Oral Diagnosis, School of Dentistry, University of Campinas (UNICAMP), Av. Limeira, 901 – Bairro Areião, CEP: 13414-903 Piracicaba, São Paulo (Brazil)
  7. (Brazil)
  8. Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo (Brazil)
Publication Date:
OSTI Identifier:
22649797
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 350; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; CARCINOMAS; CATTLE; CELL PROLIFERATION; IN VITRO; MASS SPECTROSCOPY; MIGRATION; POLYMERASE CHAIN REACTION; RECEPTORS; TRANSCRIPTION; VIABILITY

Citation Formats

Korvala, Johanna, E-mail: johanna.korvala@oulu.fi, Jee, Kowan, Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Porkola, Emmi, Almangush, Alhadi, Mosakhani, Neda, Bitu, Carolina, Cervigne, Nilva K., Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Zandonadi, Flávia S., Meirelles, Gabriela V., Leme, Adriana Franco Paes, Coletta, Ricardo D., and and others. MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro. United States: N. p., 2017. Web. doi:10.1016/J.YEXCR.2016.10.015.
Korvala, Johanna, E-mail: johanna.korvala@oulu.fi, Jee, Kowan, Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Porkola, Emmi, Almangush, Alhadi, Mosakhani, Neda, Bitu, Carolina, Cervigne, Nilva K., Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Zandonadi, Flávia S., Meirelles, Gabriela V., Leme, Adriana Franco Paes, Coletta, Ricardo D., & and others. MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro. United States. doi:10.1016/J.YEXCR.2016.10.015.
Korvala, Johanna, E-mail: johanna.korvala@oulu.fi, Jee, Kowan, Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Porkola, Emmi, Almangush, Alhadi, Mosakhani, Neda, Bitu, Carolina, Cervigne, Nilva K., Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Zandonadi, Flávia S., Meirelles, Gabriela V., Leme, Adriana Franco Paes, Coletta, Ricardo D., and and others. Sun . "MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro". United States. doi:10.1016/J.YEXCR.2016.10.015.
@article{osti_22649797,
title = {MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro},
author = {Korvala, Johanna, E-mail: johanna.korvala@oulu.fi and Jee, Kowan and Department of Pathology, Haartman Institute, University of Helsinki, Helsinki and Porkola, Emmi and Almangush, Alhadi and Mosakhani, Neda and Bitu, Carolina and Cervigne, Nilva K. and Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP and Zandonadi, Flávia S. and Meirelles, Gabriela V. and Leme, Adriana Franco Paes and Coletta, Ricardo D. and and others},
abstractNote = {Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated “Focal adhesion” and “ECM-receptor interaction” as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren’t significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.},
doi = {10.1016/J.YEXCR.2016.10.015},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 1,
volume = 350,
place = {United States},
year = {2017},
month = {1}
}