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Title: Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions

Abstract

Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. •more » A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.« less

Authors:
 [1];  [2];  [3];  [3];  [2];  [4];  [5];  [6];  [1];  [3]
  1. Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798 (Singapore)
  2. School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore)
  3. (Singapore)
  4. Bioinformatics Institute, Agency for Science Technology & Research - A*STAR, 30 Biopolis Street, Matrix, Singapore 138671 (Singapore)
  5. Division of Plastic, Reconstructive and Aesthetic Surgery, Department of Surgery, National University Hospital (NUH) and National University of Singapore (NUS), Kent Ridge Wing, Singapore 119074 (Singapore)
  6. Plastic, Reconstructive and Aesthetic Surgery Section, Tan Tock Seng Hospital (TTSH), 11, Jalan Tan Tock Seng, Singapore 308433 (Singapore)
Publication Date:
OSTI Identifier:
22649777
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 348; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; ALGINATES; BONE MARROW; CELL DIFFERENTIATION; CELL PROLIFERATION; FLEXIBILITY; GENES; IN VITRO; MATRICES; MORPHOLOGY; PLASTICS; SKELETON; STEM CELLS; TCP; TISSUE CULTURES

Citation Formats

Guneta, Vipra, Tan, Nguan Soon, KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673, Chan, Soon Kiat Jeremy, Tanavde, Vivek, Lim, Thiam Chye, Wong, Thien Chong Marcus, Choong, Cleo, E-mail: cleochoong@ntu.edu.sg, and KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899. Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.09.012.
Guneta, Vipra, Tan, Nguan Soon, KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673, Chan, Soon Kiat Jeremy, Tanavde, Vivek, Lim, Thiam Chye, Wong, Thien Chong Marcus, Choong, Cleo, E-mail: cleochoong@ntu.edu.sg, & KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899. Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions. United States. doi:10.1016/J.YEXCR.2016.09.012.
Guneta, Vipra, Tan, Nguan Soon, KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673, Chan, Soon Kiat Jeremy, Tanavde, Vivek, Lim, Thiam Chye, Wong, Thien Chong Marcus, Choong, Cleo, E-mail: cleochoong@ntu.edu.sg, and KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899. Tue . "Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions". United States. doi:10.1016/J.YEXCR.2016.09.012.
@article{osti_22649777,
title = {Comparative study of adipose-derived stem cells and bone marrow-derived stem cells in similar microenvironmental conditions},
author = {Guneta, Vipra and Tan, Nguan Soon and KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899 and Institute of Molecular and Cell Biology, Agency for Science Technology & Research - A*STAR, 61 Biopolis Drive, Proteos, Singapore 138673 and Chan, Soon Kiat Jeremy and Tanavde, Vivek and Lim, Thiam Chye and Wong, Thien Chong Marcus and Choong, Cleo, E-mail: cleochoong@ntu.edu.sg and KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899},
abstractNote = {Mesenchymal stem cells (MSCs), which were first isolated from the bone marrow, are now being extracted from various other tissues in the body, including the adipose tissue. The current study presents systematic evidence of how the adipose tissue-derived stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (Bm-MSCs) behave when cultured in specific pro-adipogenic microenvironments. The cells were first characterized and identified as MSCs in terms of their morphology, phenotypic expression, self-renewal capabilities and multi-lineage potential. Subsequently, the proliferation and gene expression profiles of the cell populations cultured on two-dimensional (2D) adipose tissue extracellular matrix (ECM)-coated tissue culture plastic (TCP) and in three-dimensional (3D) AlgiMatrix® microenvironments were analyzed. Overall, it was found that adipogenesis was triggered in both cell populations due to the presence of adipose tissue ECM. However, in 3D microenvironments, ASCs and Bm-MSCs were predisposed to the adipogenic and osteogenic lineages respectively. Overall, findings from this study will contribute to ongoing efforts in adipose tissue engineering as well as provide new insights into the role of the ECM and cues provided by the immediate microenvironment for stem cell differentiation. - Highlights: • Native adipose tissue ECM coated on 2D TCP triggers adipogenesis in both ASCs and Bm-MSCs. • A 3D microenvironment with similar stiffness to adipose tissue induces adipogenic differentiation of ASCs. • ASCs cultured in 3D alginate scaffolds exhibit predisposition to adipogenesis. • Bm-MSCs cultured in 3D alginate scaffolds exhibit predisposition to osteogenesis. • The native microenvironment of the cells affects their differentiation behaviour in vitro.},
doi = {10.1016/J.YEXCR.2016.09.012},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 348,
place = {United States},
year = {2016},
month = {11}
}