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Title: Polymer microfiber meshes facilitate cardiac differentiation of c-kit{sup +} human cardiac stem cells

Abstract

Electrospun microfiber meshes have been shown to support the proliferation and differentiation of many types of stem cells, but the phenotypic fate of c-kit{sup +} human cardiac stem cells (hCSCs) have not been explored. To this end, we utilized thin (~5 µm) elastomeric meshes consisting of aligned 1.7 µm diameter poly (ester-urethane urea) microfibers as substrates to examine their effect on hCSC viability, morphology, proliferation, and differentiation relative to cells cultured on tissue culture polystyrene (TCPS). The results showed that cells on microfiber meshes displayed an elongated morphology aligned in the direction of fiber orientation, lower proliferation rates, but increased expressions of genes and proteins majorly associated with cardiomyocyte phenotype. The early (NK2 homeobox 5, Nkx2.5) and late (cardiac troponin I, cTnI) cardiomyocyte genes were significantly increased on meshes (Nkx=2.5 56.2±13.0, cTnl=2.9±0.56,) over TCPS (Nkx2.5=4.2±0.9, cTnl=1.6±0.5, n=9, p<0.05 for both groups) after differentiation. In contrast, expressions of smooth muscle markers, Gata6 and myosin heavy chain (SM-MHC), were decreased on meshes. Immunocytochemical analysis with cardiac antibody exhibited the similar pattern of above cardiac differentiation. We conclude that aligned microfiber meshes are suitable for guiding cardiac differentiation of hCSCs and may facilitate stem cell-based therapies for treatment of cardiac diseases. - Highlights:more » • First study to characterize c-kit{sup +} human cardiac stem cells on microfiber meshes. • Microfiber meshes seem reducing cell proliferation, but no effect on cell viability. • Microfiber meshes facilitate the elongation of human cardiac stem cells in culture. • Cardiac but not smooth muscle differentiation were enhanced on microfiber meshes. • Microfiber meshes may be used as cardiac patches in cell-based cardiac therapy.« less

Authors:
 [1];  [2];  [3]; ;  [1];  [2];  [4];  [1]
  1. Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA (United States)
  2. Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA (United States)
  3. Research Institute of Heart Failure, Shanghai East Hospital of Tongji University, Shanghai (China)
  4. School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA (United States)
Publication Date:
OSTI Identifier:
22649757
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 347; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; ANIMAL TISSUES; ANTIBODIES; ANTIGENS; CELL CULTURES; CELL PROLIFERATION; DISEASES; GLYCEROL; GROWTH FACTORS; MATRICES; MUSCLES; MYOSIN; ORIENTATION; PHENOTYPE; PHOSPHOTRANSFERASES; PLANT GROWTH; PLANT TISSUES; POLYSTYRENE; PROLIFERATION; RECEPTORS; STEM CELLS; SUBSTRATES; THERAPY; TISSUE CULTURES; UREA; URETHANE

Citation Formats

Kan, Lijuan, Thayer, Patrick, Fan, Huimin, Ledford, Benjamin, Chen, Miao, Goldstein, Aaron, Cao, Guohua, and He, Jia-Qiang, E-mail: jiahe@vt.edu. Polymer microfiber meshes facilitate cardiac differentiation of c-kit{sup +} human cardiac stem cells. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.07.024.
Kan, Lijuan, Thayer, Patrick, Fan, Huimin, Ledford, Benjamin, Chen, Miao, Goldstein, Aaron, Cao, Guohua, & He, Jia-Qiang, E-mail: jiahe@vt.edu. Polymer microfiber meshes facilitate cardiac differentiation of c-kit{sup +} human cardiac stem cells. United States. doi:10.1016/J.YEXCR.2016.07.024.
Kan, Lijuan, Thayer, Patrick, Fan, Huimin, Ledford, Benjamin, Chen, Miao, Goldstein, Aaron, Cao, Guohua, and He, Jia-Qiang, E-mail: jiahe@vt.edu. Sat . "Polymer microfiber meshes facilitate cardiac differentiation of c-kit{sup +} human cardiac stem cells". United States. doi:10.1016/J.YEXCR.2016.07.024.
@article{osti_22649757,
title = {Polymer microfiber meshes facilitate cardiac differentiation of c-kit{sup +} human cardiac stem cells},
author = {Kan, Lijuan and Thayer, Patrick and Fan, Huimin and Ledford, Benjamin and Chen, Miao and Goldstein, Aaron and Cao, Guohua and He, Jia-Qiang, E-mail: jiahe@vt.edu},
abstractNote = {Electrospun microfiber meshes have been shown to support the proliferation and differentiation of many types of stem cells, but the phenotypic fate of c-kit{sup +} human cardiac stem cells (hCSCs) have not been explored. To this end, we utilized thin (~5 µm) elastomeric meshes consisting of aligned 1.7 µm diameter poly (ester-urethane urea) microfibers as substrates to examine their effect on hCSC viability, morphology, proliferation, and differentiation relative to cells cultured on tissue culture polystyrene (TCPS). The results showed that cells on microfiber meshes displayed an elongated morphology aligned in the direction of fiber orientation, lower proliferation rates, but increased expressions of genes and proteins majorly associated with cardiomyocyte phenotype. The early (NK2 homeobox 5, Nkx2.5) and late (cardiac troponin I, cTnI) cardiomyocyte genes were significantly increased on meshes (Nkx=2.5 56.2±13.0, cTnl=2.9±0.56,) over TCPS (Nkx2.5=4.2±0.9, cTnl=1.6±0.5, n=9, p<0.05 for both groups) after differentiation. In contrast, expressions of smooth muscle markers, Gata6 and myosin heavy chain (SM-MHC), were decreased on meshes. Immunocytochemical analysis with cardiac antibody exhibited the similar pattern of above cardiac differentiation. We conclude that aligned microfiber meshes are suitable for guiding cardiac differentiation of hCSCs and may facilitate stem cell-based therapies for treatment of cardiac diseases. - Highlights: • First study to characterize c-kit{sup +} human cardiac stem cells on microfiber meshes. • Microfiber meshes seem reducing cell proliferation, but no effect on cell viability. • Microfiber meshes facilitate the elongation of human cardiac stem cells in culture. • Cardiac but not smooth muscle differentiation were enhanced on microfiber meshes. • Microfiber meshes may be used as cardiac patches in cell-based cardiac therapy.},
doi = {10.1016/J.YEXCR.2016.07.024},
journal = {Experimental Cell Research},
number = 1,
volume = 347,
place = {United States},
year = {Sat Sep 10 00:00:00 EDT 2016},
month = {Sat Sep 10 00:00:00 EDT 2016}
}