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Title: Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells

Abstract

Purpose: Understanding the DNA damage and repair induced by hadron therapy (HT) beams is crucial for developing novel strategies to maximize the use of HT beams to treat cancer patients. However, spatiotemporal studies of DNA damage and repair for beam energies relevant to HT have been challenging. We report a technique that enables spatiotemporal measurement of radiation-induced damage in live cells and colocalization of this damage with charged particle tracks over a broad range of clinically relevant beam energies. The technique uses novel fluorescence nuclear track detectors with fluorescence confocal laser scanning microscopy in the beam line to visualize particle track traversals within the subcellular compartments of live cells within seconds after injury. Methods and Materials: We designed and built a portable fluorescence confocal laser scanning microscope for use in the beam path, coated fluorescence nuclear track detectors with fluorescent-tagged live cells (HT1080 expressing enhanced green fluorescent protein tagged to XRCC1, a single-strand break repair protein), placed the entire assembly into a proton therapy beam line, and irradiated the cells with a fluence of ∼1 × 10{sup 6} protons/cm{sup 2}. Results: We successfully obtained confocal images of proton tracks and foci of DNA single-strand breaks immediately after irradiation. Conclusions: This technique representsmore » an innovative method for analyzing biological responses in any HT beam line at energies and dose rates relevant to therapy. It allows precise determination of the number of tracks traversing a subcellular compartment and monitoring the cellular damage therein, and has the potential to measure the linear energy transfer of each track from therapeutic beams.« less

Authors:
 [1];  [1];  [2];  [1];  [2];  [3];  [4];  [1];  [5];  [1];  [2]
  1. Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States)
  2. (United States)
  3. Department of Medical Physics, The Ottawa Hospital Cancer Centre, Ottawa, Ontario (Canada)
  4. Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Centre, Dallas, Texas (United States)
  5. Crystal Growth Division, Landauer, Inc, Stillwater, Oklahoma (United States)
Publication Date:
OSTI Identifier:
22648800
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 96; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CHARGED PARTICLES; DAMAGE; DNA REPAIR; DOSE RATES; FLUORESCENCE; FLUORINE COMPOUNDS; LIVER; PARTICLE TRACKS; PROTON BEAMS; RADIOTHERAPY; STRAND BREAKS

Citation Formats

McFadden, Conor H., Hallacy, Timothy M., Department of Physics and Astronomy, Rice University, Houston, Texas, Flint, David B., Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas, Granville, Dal A., Asaithamby, Aroumougame, Sahoo, Narayan, Akselrod, Mark S., Sawakuchi, Gabriel O., E-mail: gsawakuchi@mdanderson.org, and Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas. Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells. United States: N. p., 2016. Web. doi:10.1016/J.IJROBP.2016.04.007.
McFadden, Conor H., Hallacy, Timothy M., Department of Physics and Astronomy, Rice University, Houston, Texas, Flint, David B., Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas, Granville, Dal A., Asaithamby, Aroumougame, Sahoo, Narayan, Akselrod, Mark S., Sawakuchi, Gabriel O., E-mail: gsawakuchi@mdanderson.org, & Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas. Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells. United States. doi:10.1016/J.IJROBP.2016.04.007.
McFadden, Conor H., Hallacy, Timothy M., Department of Physics and Astronomy, Rice University, Houston, Texas, Flint, David B., Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas, Granville, Dal A., Asaithamby, Aroumougame, Sahoo, Narayan, Akselrod, Mark S., Sawakuchi, Gabriel O., E-mail: gsawakuchi@mdanderson.org, and Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas. Thu . "Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells". United States. doi:10.1016/J.IJROBP.2016.04.007.
@article{osti_22648800,
title = {Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells},
author = {McFadden, Conor H. and Hallacy, Timothy M. and Department of Physics and Astronomy, Rice University, Houston, Texas and Flint, David B. and Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas and Granville, Dal A. and Asaithamby, Aroumougame and Sahoo, Narayan and Akselrod, Mark S. and Sawakuchi, Gabriel O., E-mail: gsawakuchi@mdanderson.org and Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas},
abstractNote = {Purpose: Understanding the DNA damage and repair induced by hadron therapy (HT) beams is crucial for developing novel strategies to maximize the use of HT beams to treat cancer patients. However, spatiotemporal studies of DNA damage and repair for beam energies relevant to HT have been challenging. We report a technique that enables spatiotemporal measurement of radiation-induced damage in live cells and colocalization of this damage with charged particle tracks over a broad range of clinically relevant beam energies. The technique uses novel fluorescence nuclear track detectors with fluorescence confocal laser scanning microscopy in the beam line to visualize particle track traversals within the subcellular compartments of live cells within seconds after injury. Methods and Materials: We designed and built a portable fluorescence confocal laser scanning microscope for use in the beam path, coated fluorescence nuclear track detectors with fluorescent-tagged live cells (HT1080 expressing enhanced green fluorescent protein tagged to XRCC1, a single-strand break repair protein), placed the entire assembly into a proton therapy beam line, and irradiated the cells with a fluence of ∼1 × 10{sup 6} protons/cm{sup 2}. Results: We successfully obtained confocal images of proton tracks and foci of DNA single-strand breaks immediately after irradiation. Conclusions: This technique represents an innovative method for analyzing biological responses in any HT beam line at energies and dose rates relevant to therapy. It allows precise determination of the number of tracks traversing a subcellular compartment and monitoring the cellular damage therein, and has the potential to measure the linear energy transfer of each track from therapeutic beams.},
doi = {10.1016/J.IJROBP.2016.04.007},
journal = {International Journal of Radiation Oncology, Biology and Physics},
number = 1,
volume = 96,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2016},
month = {Thu Sep 01 00:00:00 EDT 2016}
}