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Title: β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes

Abstract

Myogenesis is required for the development of skeletal muscle. Accumulating evidence indicates that the expression of several genes are upregulated during myogenesis and these genes play pivotal roles in myogenesis. However, the molecular mechanism underlying myogenesis is not fully understood. In this study, we found that β-taxilin, which is specifically expressed in the skeletal muscle and heart tissues, was progressively expressed during differentiation of C2C12 myoblasts into myotubes, prompting us to investigate the role of β-taxilin in myogenesis. In C2C12 cells, knockdown of β-taxilin impaired the fusion of myoblasts into myotubes, and decreased the diameter of myotubes. We also found that β-taxilin interacted with dysbindin, a coiled-coil-containing protein. Knockdown of dysbindin conversely promoted the fusion of myoblasts into myotubes and increased the diameter of myotubes in C2C12 cells. Furthermore, knockdown of dysbindin attenuated the inhibitory effect of β-taxilin depletion on myotube formation of C2C12 cells. These results demonstrate that β-taxilin participates in myogenesis through suppressing the function of dysbindin to inhibit the differentiation of C2C12 myoblasts into myotubes. - Highlights: • β‐Taxilin is progressively expressed during differentiation of C2C12 cell. • Knockdown of β-taxilin impaired C2C12 myotube formation. • β‐Taxilin interacted with dysbindin. • Knockdown of dysbindin promoted C2C12 myotubemore » formation. • The function of β-taxilin in C2C12 myotube formation depends on dysbindin.« less

Authors:
; ; ;  [1];  [2];  [1]
  1. Department of Molecular and Cell Biology, Graduate school of Medicine, Dokkyo Medical University, 880 Kitakobayashi, Mibu-town, Tochigi 321-0293 (Japan)
  2. Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292 (Japan)
Publication Date:
OSTI Identifier:
22648599
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 345; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; FLUORESCENCE; FUNCTIONS; GENES; HEART; MEMBRANES; MYOBLASTS; MYOSIN; OXIDOREDUCTASES; PHOSPHATES; PLANT TISSUES; RECEPTORS; RNA; SORTING; TRANSCRIPTION; TRANSFERRIN

Citation Formats

Sakane, Hiroshi, Makiyama, Tomohiko, Nogami, Satoru, Horii, Yukimi, Akasaki, Kenji, and Shirataki, Hiromichi, E-mail: hiro-sh@dokkyomed.ac.jp. β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.05.016.
Sakane, Hiroshi, Makiyama, Tomohiko, Nogami, Satoru, Horii, Yukimi, Akasaki, Kenji, & Shirataki, Hiromichi, E-mail: hiro-sh@dokkyomed.ac.jp. β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes. United States. doi:10.1016/J.YEXCR.2016.05.016.
Sakane, Hiroshi, Makiyama, Tomohiko, Nogami, Satoru, Horii, Yukimi, Akasaki, Kenji, and Shirataki, Hiromichi, E-mail: hiro-sh@dokkyomed.ac.jp. Fri . "β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes". United States. doi:10.1016/J.YEXCR.2016.05.016.
@article{osti_22648599,
title = {β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes},
author = {Sakane, Hiroshi and Makiyama, Tomohiko and Nogami, Satoru and Horii, Yukimi and Akasaki, Kenji and Shirataki, Hiromichi, E-mail: hiro-sh@dokkyomed.ac.jp},
abstractNote = {Myogenesis is required for the development of skeletal muscle. Accumulating evidence indicates that the expression of several genes are upregulated during myogenesis and these genes play pivotal roles in myogenesis. However, the molecular mechanism underlying myogenesis is not fully understood. In this study, we found that β-taxilin, which is specifically expressed in the skeletal muscle and heart tissues, was progressively expressed during differentiation of C2C12 myoblasts into myotubes, prompting us to investigate the role of β-taxilin in myogenesis. In C2C12 cells, knockdown of β-taxilin impaired the fusion of myoblasts into myotubes, and decreased the diameter of myotubes. We also found that β-taxilin interacted with dysbindin, a coiled-coil-containing protein. Knockdown of dysbindin conversely promoted the fusion of myoblasts into myotubes and increased the diameter of myotubes in C2C12 cells. Furthermore, knockdown of dysbindin attenuated the inhibitory effect of β-taxilin depletion on myotube formation of C2C12 cells. These results demonstrate that β-taxilin participates in myogenesis through suppressing the function of dysbindin to inhibit the differentiation of C2C12 myoblasts into myotubes. - Highlights: • β‐Taxilin is progressively expressed during differentiation of C2C12 cell. • Knockdown of β-taxilin impaired C2C12 myotube formation. • β‐Taxilin interacted with dysbindin. • Knockdown of dysbindin promoted C2C12 myotube formation. • The function of β-taxilin in C2C12 myotube formation depends on dysbindin.},
doi = {10.1016/J.YEXCR.2016.05.016},
journal = {Experimental Cell Research},
number = 2,
volume = 345,
place = {United States},
year = {Fri Jul 15 00:00:00 EDT 2016},
month = {Fri Jul 15 00:00:00 EDT 2016}
}