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Title: Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway

Abstract

This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway. - Highlights: • Hyaluronic acid (HA) could promote the proliferation of hAMSCs. • HA treatment dose not affect the pluripotency of hAMSCs. • HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling.

Authors:
; ; ; ;  [1];  [2];  [1]
  1. Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000 (China)
  2. State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240 (China)
Publication Date:
OSTI Identifier:
22648598
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 345; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; DOSES; HUMAN POPULATIONS; HYALURONIC ACID; MESSENGER-RNA; PLANT GROWTH; POTASSIUM IODIDES; PROTEINS; SIGNALS; STEM CELLS; TIME DEPENDENCE

Citation Formats

Liu, Ru-Ming, Sun, Ren-Gang, Zhang, Ling-Tao, Zhang, Qing-Fang, Chen, Dai-Xiong, Zhong, Jian-Jiang, E-mail: jjzhong@sjtu.edu.cn, and Xiao, Jian-Hui, E-mail: jhxiao@yahoo.com. Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.05.019.
Liu, Ru-Ming, Sun, Ren-Gang, Zhang, Ling-Tao, Zhang, Qing-Fang, Chen, Dai-Xiong, Zhong, Jian-Jiang, E-mail: jjzhong@sjtu.edu.cn, & Xiao, Jian-Hui, E-mail: jhxiao@yahoo.com. Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway. United States. doi:10.1016/J.YEXCR.2016.05.019.
Liu, Ru-Ming, Sun, Ren-Gang, Zhang, Ling-Tao, Zhang, Qing-Fang, Chen, Dai-Xiong, Zhong, Jian-Jiang, E-mail: jjzhong@sjtu.edu.cn, and Xiao, Jian-Hui, E-mail: jhxiao@yahoo.com. Fri . "Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway". United States. doi:10.1016/J.YEXCR.2016.05.019.
@article{osti_22648598,
title = {Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway},
author = {Liu, Ru-Ming and Sun, Ren-Gang and Zhang, Ling-Tao and Zhang, Qing-Fang and Chen, Dai-Xiong and Zhong, Jian-Jiang, E-mail: jjzhong@sjtu.edu.cn and Xiao, Jian-Hui, E-mail: jhxiao@yahoo.com},
abstractNote = {This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway. - Highlights: • Hyaluronic acid (HA) could promote the proliferation of hAMSCs. • HA treatment dose not affect the pluripotency of hAMSCs. • HA increases hAMSCs proliferation through activation of Wnt/β-catenin signaling.},
doi = {10.1016/J.YEXCR.2016.05.019},
journal = {Experimental Cell Research},
number = 2,
volume = 345,
place = {United States},
year = {Fri Jul 15 00:00:00 EDT 2016},
month = {Fri Jul 15 00:00:00 EDT 2016}
}