skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Wharton's jelly mesenchymal stromal cells have contrasting effects on proliferation and phenotype of cancer stem cells from different subtypes of lung cancer

Abstract

Studies on the role of multipotent mesenchymal stromal cells (MSC) on tumor growth have reported both a tumor promoting and a suppressive effect. The aim of the present study was to determine the effect of MSC isolated from Wharton's jelly of umbilical cord (WJMSC) on lung cancer stem cells (LCSC) derived from human lung tumors: two adenocarcinomas (AC) and two squamous cell carcinomas (SCC). LCSC derived from SCC and AC expressed, to varying extents, the more relevant stem cell markers. The effect of WJMSC on LCSC was investigated in vitro using conditioned medium (WJ-CM): a proliferation increase in AC-LCSC was observed, with an increase in the ALDH+ and in the CD133+ cell population. By contrast, WJ-CM hampered the growth of SCC-LCSC, with an increase in the pre-G1 phase indicating the induction of apoptosis. Furthermore, the ALDH+ and CD133+ population was also reduced. In vivo, subcutaneous co-transplantation of AC-LCSC/WJMSC generated larger tumors than AC-LCSC alone, characterized by an increased percentage of CD133+ and CD166+ cells. By contrast, co-transplantation of WJMSC and SCC-LCSC did not affect the tumor size. Our results strongly suggest that WJMSC exert, both in vitro and in vivo, contrasting effects on LCSC derived from different lung tumor subtypes.more » - Highlights: • CM from WJMSC induces apoptosis of SCC-LCSC and reduction of ALDH+ and CD133+ cells. • Specificity of SCC-LCSC inhibition by WJ-CM is proved by the use of a CM from NHDF. • WJ-CM enhance AC-LCSC proliferation and increase CD133+ and ALDH+ cell fractions. • Coinjection of WJMSC with AC-LCSC increase tumor growth with SCC-LCSC has no effect.« less

Authors:
 [1];  [1];  [2];  [1];  [3];  [4];  [1];  [5];  [6];  [7];  [1];
  1. Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome (Italy)
  2. Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila (Italy)
  3. Regina Elena National Cancer Institute, Rome (Italy)
  4. Faculty of Veterinary Medicine, University of Teramo (Italy)
  5. Experimental Animal Welfare Sector of the Istituto Superiore di Sanità, Rome (Italy)
  6. Department of Gynecology, Obstetrics and Urological Sciences, Sapienza University of Rome (Italy)
  7. Institute of Cell Biology and Neurobiology, National Research Council, Rome (Italy)
Publication Date:
OSTI Identifier:
22648597
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 345; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CARCINOMAS; DECARBOXYLASES; EOSIN; FIBROBLASTS; FLUORESCEIN; GLYCINE; HEMATOXYLIN; HUMAN POPULATIONS; IN VITRO; IN VIVO; INDEXES; INHIBITION; ISOTHIOCYANATES; LUNGS; OXIDOREDUCTASES; PHENOTYPE; PHOSPHATES; PLANT GROWTH; PROLIFERATION; SPECIFICITY; STEM CELLS

Citation Formats

Vulcano, Francesca, E-mail: francesca.vulcano@iss.it, Milazzo, Luisa, E-mail: luisa.milazzo@iss.it, Ciccarelli, Carmela, E-mail: carmela.ciccarelli@univaq.it, Eramo, Adriana, E-mail: adriana.eramo@iss.it, Sette, Giovanni, E-mail: giovanni.sette@gmail.com, Mauro, Annunziata, E-mail: amauro@unite.it, Macioce, Giampiero, E-mail: giampiero.macioce@iss.it, Martinelli, Andrea, E-mail: andrea.martinelli@iss.it, La Torre, Renato, E-mail: renato.latorre@uniroma1.it, Casalbore, Patrizia, E-mail: patrizia.casalbore@cnr.it, Hassan, Hamisa Jane, E-mail: jane.hassan@iss.it, and and others. Wharton's jelly mesenchymal stromal cells have contrasting effects on proliferation and phenotype of cancer stem cells from different subtypes of lung cancer. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.06.003.
Vulcano, Francesca, E-mail: francesca.vulcano@iss.it, Milazzo, Luisa, E-mail: luisa.milazzo@iss.it, Ciccarelli, Carmela, E-mail: carmela.ciccarelli@univaq.it, Eramo, Adriana, E-mail: adriana.eramo@iss.it, Sette, Giovanni, E-mail: giovanni.sette@gmail.com, Mauro, Annunziata, E-mail: amauro@unite.it, Macioce, Giampiero, E-mail: giampiero.macioce@iss.it, Martinelli, Andrea, E-mail: andrea.martinelli@iss.it, La Torre, Renato, E-mail: renato.latorre@uniroma1.it, Casalbore, Patrizia, E-mail: patrizia.casalbore@cnr.it, Hassan, Hamisa Jane, E-mail: jane.hassan@iss.it, & and others. Wharton's jelly mesenchymal stromal cells have contrasting effects on proliferation and phenotype of cancer stem cells from different subtypes of lung cancer. United States. doi:10.1016/J.YEXCR.2016.06.003.
Vulcano, Francesca, E-mail: francesca.vulcano@iss.it, Milazzo, Luisa, E-mail: luisa.milazzo@iss.it, Ciccarelli, Carmela, E-mail: carmela.ciccarelli@univaq.it, Eramo, Adriana, E-mail: adriana.eramo@iss.it, Sette, Giovanni, E-mail: giovanni.sette@gmail.com, Mauro, Annunziata, E-mail: amauro@unite.it, Macioce, Giampiero, E-mail: giampiero.macioce@iss.it, Martinelli, Andrea, E-mail: andrea.martinelli@iss.it, La Torre, Renato, E-mail: renato.latorre@uniroma1.it, Casalbore, Patrizia, E-mail: patrizia.casalbore@cnr.it, Hassan, Hamisa Jane, E-mail: jane.hassan@iss.it, and and others. Fri . "Wharton's jelly mesenchymal stromal cells have contrasting effects on proliferation and phenotype of cancer stem cells from different subtypes of lung cancer". United States. doi:10.1016/J.YEXCR.2016.06.003.
@article{osti_22648597,
title = {Wharton's jelly mesenchymal stromal cells have contrasting effects on proliferation and phenotype of cancer stem cells from different subtypes of lung cancer},
author = {Vulcano, Francesca, E-mail: francesca.vulcano@iss.it and Milazzo, Luisa, E-mail: luisa.milazzo@iss.it and Ciccarelli, Carmela, E-mail: carmela.ciccarelli@univaq.it and Eramo, Adriana, E-mail: adriana.eramo@iss.it and Sette, Giovanni, E-mail: giovanni.sette@gmail.com and Mauro, Annunziata, E-mail: amauro@unite.it and Macioce, Giampiero, E-mail: giampiero.macioce@iss.it and Martinelli, Andrea, E-mail: andrea.martinelli@iss.it and La Torre, Renato, E-mail: renato.latorre@uniroma1.it and Casalbore, Patrizia, E-mail: patrizia.casalbore@cnr.it and Hassan, Hamisa Jane, E-mail: jane.hassan@iss.it and and others},
abstractNote = {Studies on the role of multipotent mesenchymal stromal cells (MSC) on tumor growth have reported both a tumor promoting and a suppressive effect. The aim of the present study was to determine the effect of MSC isolated from Wharton's jelly of umbilical cord (WJMSC) on lung cancer stem cells (LCSC) derived from human lung tumors: two adenocarcinomas (AC) and two squamous cell carcinomas (SCC). LCSC derived from SCC and AC expressed, to varying extents, the more relevant stem cell markers. The effect of WJMSC on LCSC was investigated in vitro using conditioned medium (WJ-CM): a proliferation increase in AC-LCSC was observed, with an increase in the ALDH+ and in the CD133+ cell population. By contrast, WJ-CM hampered the growth of SCC-LCSC, with an increase in the pre-G1 phase indicating the induction of apoptosis. Furthermore, the ALDH+ and CD133+ population was also reduced. In vivo, subcutaneous co-transplantation of AC-LCSC/WJMSC generated larger tumors than AC-LCSC alone, characterized by an increased percentage of CD133+ and CD166+ cells. By contrast, co-transplantation of WJMSC and SCC-LCSC did not affect the tumor size. Our results strongly suggest that WJMSC exert, both in vitro and in vivo, contrasting effects on LCSC derived from different lung tumor subtypes. - Highlights: • CM from WJMSC induces apoptosis of SCC-LCSC and reduction of ALDH+ and CD133+ cells. • Specificity of SCC-LCSC inhibition by WJ-CM is proved by the use of a CM from NHDF. • WJ-CM enhance AC-LCSC proliferation and increase CD133+ and ALDH+ cell fractions. • Coinjection of WJMSC with AC-LCSC increase tumor growth with SCC-LCSC has no effect.},
doi = {10.1016/J.YEXCR.2016.06.003},
journal = {Experimental Cell Research},
number = 2,
volume = 345,
place = {United States},
year = {Fri Jul 15 00:00:00 EDT 2016},
month = {Fri Jul 15 00:00:00 EDT 2016}
}