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Title: SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts

Abstract

Mouse embryo fibroblasts (MEFs) grow slowly after cultivation from animals, however, after an extended period of cultivation, their growth accelerates. We found that SWAP-70 deficient MEFs failed to increase growth rates. They maintain normal growth rates and proliferation cycles for at least 5 years. Complementing SWAP-70 deficiency in one of these MEF clones, MEF1F2, by expressing human SWAP-70 resulted in fast growth of the cells after further cultivation for a long period. The resulting cells show a transformation phenotype, since they grow on top of each other and do not show contact inhibition. This phenotype was reverted when sanguinarine, a putative SWAP-70 inhibitor, was added. Two SWAP-70 expressing clones were examined in detail. Even after cell density became very high their cdc2 and NFκB were still activated suggesting that they do not stop growing. One of the clones formed colonies in soft agar and formed tumors in nude mice. Lately, one more clone became transformed being able to make colonies in soft agar. We maintain 4 human SWAP-70 expressing MEF1F2 cell lines. Three out of 4 clones exhibited transforming phenotypes. The mouse SWAP-70 gene also promoted transformation of MEFs. Taken together our data suggest that SWAP-70 is not a typicalmore » oncogene, but is required for spontaneous transformation of MEFs. - Highlights: • Mouse embryo fibroblasts (MEFs) lacking SWAP-70 do not cause spontaneous transform. • Adding back of SWAP-70 to SWAP-70-deficient MEFs induces spontaneous transformation. • SWAP-70 is required for spontaneous transformation of MEFs.« less

Authors:
; ; ; ;  [1]; ;  [2];  [3];  [1]
  1. Institute of Cellular and System Medicine National Health Research Institute, Zhunan Town 35053, Miaoli County, Taiwan, ROC (China)
  2. Division of Tumor and Cellular Biochemistry Department of Medical Sciences Faculty of Medicine University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki-shi, Miyazaki 889-1692 Japan (Japan)
  3. Faculty of Medicine Carl Gustav Carus, Institute of Physiological Chemistry, Dresden University of Technology, Dresden (Germany)
Publication Date:
OSTI Identifier:
22648594
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 345; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGAR; CELL PROLIFERATION; COLONY FORMATION; COMPLEMENT; CULTIVATION; EMBRYOS; FIBROBLASTS; HUMAN POPULATIONS; INHIBITION; MICE; NEOPLASMS; ONCOGENES; PHENOTYPE; PLANT GROWTH; TRANSFORMATIONS

Citation Formats

Chang, Yu-Tzu, Shu, Chung-Li, Lai, Jing-Yang, Lin, Ching-Yu, Chuu, Chih-Pin, Morishita, Kazuhiro, Ichikawa, Tomonaga, Jessberger, Rolf, and Fukui, Yasuhisa, E-mail: 990412@nhri.org.tw. SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2015.06.011.
Chang, Yu-Tzu, Shu, Chung-Li, Lai, Jing-Yang, Lin, Ching-Yu, Chuu, Chih-Pin, Morishita, Kazuhiro, Ichikawa, Tomonaga, Jessberger, Rolf, & Fukui, Yasuhisa, E-mail: 990412@nhri.org.tw. SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts. United States. doi:10.1016/J.YEXCR.2015.06.011.
Chang, Yu-Tzu, Shu, Chung-Li, Lai, Jing-Yang, Lin, Ching-Yu, Chuu, Chih-Pin, Morishita, Kazuhiro, Ichikawa, Tomonaga, Jessberger, Rolf, and Fukui, Yasuhisa, E-mail: 990412@nhri.org.tw. Fri . "SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts". United States. doi:10.1016/J.YEXCR.2015.06.011.
@article{osti_22648594,
title = {SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts},
author = {Chang, Yu-Tzu and Shu, Chung-Li and Lai, Jing-Yang and Lin, Ching-Yu and Chuu, Chih-Pin and Morishita, Kazuhiro and Ichikawa, Tomonaga and Jessberger, Rolf and Fukui, Yasuhisa, E-mail: 990412@nhri.org.tw},
abstractNote = {Mouse embryo fibroblasts (MEFs) grow slowly after cultivation from animals, however, after an extended period of cultivation, their growth accelerates. We found that SWAP-70 deficient MEFs failed to increase growth rates. They maintain normal growth rates and proliferation cycles for at least 5 years. Complementing SWAP-70 deficiency in one of these MEF clones, MEF1F2, by expressing human SWAP-70 resulted in fast growth of the cells after further cultivation for a long period. The resulting cells show a transformation phenotype, since they grow on top of each other and do not show contact inhibition. This phenotype was reverted when sanguinarine, a putative SWAP-70 inhibitor, was added. Two SWAP-70 expressing clones were examined in detail. Even after cell density became very high their cdc2 and NFκB were still activated suggesting that they do not stop growing. One of the clones formed colonies in soft agar and formed tumors in nude mice. Lately, one more clone became transformed being able to make colonies in soft agar. We maintain 4 human SWAP-70 expressing MEF1F2 cell lines. Three out of 4 clones exhibited transforming phenotypes. The mouse SWAP-70 gene also promoted transformation of MEFs. Taken together our data suggest that SWAP-70 is not a typical oncogene, but is required for spontaneous transformation of MEFs. - Highlights: • Mouse embryo fibroblasts (MEFs) lacking SWAP-70 do not cause spontaneous transform. • Adding back of SWAP-70 to SWAP-70-deficient MEFs induces spontaneous transformation. • SWAP-70 is required for spontaneous transformation of MEFs.},
doi = {10.1016/J.YEXCR.2015.06.011},
journal = {Experimental Cell Research},
number = 2,
volume = 345,
place = {United States},
year = {Fri Jul 15 00:00:00 EDT 2016},
month = {Fri Jul 15 00:00:00 EDT 2016}
}