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Title: The therapeutic effects of docosahexaenoic acid on oestrogen/androgen-induced benign prostatic hyperplasia in rats

Abstract

Benign prostatic hyperplasia (BPH) is one of the major disorders of the urinary system in elderly men. Docosahexaenoic acid (DHA) is the main component of n-3 polyunsaturated fatty acids (n-3 PUFAs) and has nerve protective, anti-inflammatory and tumour-growth inhibitory effects. Here, the therapeutic potential of DHA in treating BPH was investigated. Seal oil effectively prevented the development of prostatic hyperplasia induced by oestradiol/testosterone in a rat model by suppressing the increase of the prostatic index (PI), reducing the thickness of the peri-glandular smooth muscle layer, inhibiting the proliferation of both prostate epithelial and stromal cells, and downregulating the expression of androgen receptor (AR) and oestrogen receptor α (ERα). An in vitro study showed that DHA inhibited the growth of the human prostate stromal cell line WPMY-1 and the epithelial cell line RWPE-1 in a dose- and time-dependent manner. In both cell lines, the DHA arrested the cell cycle in the G2/M phase. In addition, DHA also reduced the expression of ERα and AR in the WPMY-1 and RWPE-1 cells. These results indicate that DHA inhibits the multiplication of prostate stromal and epithelial cells through a mechanism that may involve cell cycle arrest and the downregulation of ERα and AR expression.more » - Highlights: • Seal oil prevents oestradiol/testosterone (E2/T)-induced BPH in castrated rats. • Seal oil downregulates the expression of oestrogen receptor α(ERα) and androgen receptor (AR) in rat BPH tissues. • DHA inhibits the growth of human prostate stromal and epithelial cells in vitro. • DHA arrests human prostate stromal and epithelial cells in the G2/M phase and downregulates the expression of cyclin B1. • DHA inhibits the expression of ERα and AR in human prostate stromal and epithelial cells.« less

Authors:
 [1];  [2]; ; ; ;  [1];  [2];  [3];  [4];  [1]
  1. Department of Biochemistry and Molecular Biology, College of Life Sciences, Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin 300071 (China)
  2. Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211 (China)
  3. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 (China)
  4. Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070 (China)
Publication Date:
OSTI Identifier:
22648592
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 345; Journal Issue: 2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; ANIMAL TISSUES; ANTIGENS; BPH; CARBOXYLIC ACIDS; CELL CYCLE; DOSES; ESTROGENS; GTP-ASES; HUMAN POPULATIONS; HYPOXANTHINE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; IN VITRO; INDEXES; INFLAMMATION; LAYERS; MEN; MUSCLES; NEOPLASMS; NERVES; OILS; OXIDOREDUCTASES; PHOSPHATES; PLANT GROWTH; PLANT TISSUES; POTENTIALS; PROLIFERATION; PROSTATE; RATS; RECEPTORS; SEALS; TESTOSTERONE; THICKNESS; TIME DEPENDENCE

Citation Formats

Wang, Chao, Luo, Fei, Zhou, Ying, Du, Xiaoling, Shi, Jiandang, Zhao, Xiaoling, Xu, Yong, Zhu, Yan, Hong, Wei, E-mail: hongwei@tijmu.edu.cn, and Zhang, Ju, E-mail: zhangju@nankai.edu.cn. The therapeutic effects of docosahexaenoic acid on oestrogen/androgen-induced benign prostatic hyperplasia in rats. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2015.03.026.
Wang, Chao, Luo, Fei, Zhou, Ying, Du, Xiaoling, Shi, Jiandang, Zhao, Xiaoling, Xu, Yong, Zhu, Yan, Hong, Wei, E-mail: hongwei@tijmu.edu.cn, & Zhang, Ju, E-mail: zhangju@nankai.edu.cn. The therapeutic effects of docosahexaenoic acid on oestrogen/androgen-induced benign prostatic hyperplasia in rats. United States. doi:10.1016/J.YEXCR.2015.03.026.
Wang, Chao, Luo, Fei, Zhou, Ying, Du, Xiaoling, Shi, Jiandang, Zhao, Xiaoling, Xu, Yong, Zhu, Yan, Hong, Wei, E-mail: hongwei@tijmu.edu.cn, and Zhang, Ju, E-mail: zhangju@nankai.edu.cn. Fri . "The therapeutic effects of docosahexaenoic acid on oestrogen/androgen-induced benign prostatic hyperplasia in rats". United States. doi:10.1016/J.YEXCR.2015.03.026.
@article{osti_22648592,
title = {The therapeutic effects of docosahexaenoic acid on oestrogen/androgen-induced benign prostatic hyperplasia in rats},
author = {Wang, Chao and Luo, Fei and Zhou, Ying and Du, Xiaoling and Shi, Jiandang and Zhao, Xiaoling and Xu, Yong and Zhu, Yan and Hong, Wei, E-mail: hongwei@tijmu.edu.cn and Zhang, Ju, E-mail: zhangju@nankai.edu.cn},
abstractNote = {Benign prostatic hyperplasia (BPH) is one of the major disorders of the urinary system in elderly men. Docosahexaenoic acid (DHA) is the main component of n-3 polyunsaturated fatty acids (n-3 PUFAs) and has nerve protective, anti-inflammatory and tumour-growth inhibitory effects. Here, the therapeutic potential of DHA in treating BPH was investigated. Seal oil effectively prevented the development of prostatic hyperplasia induced by oestradiol/testosterone in a rat model by suppressing the increase of the prostatic index (PI), reducing the thickness of the peri-glandular smooth muscle layer, inhibiting the proliferation of both prostate epithelial and stromal cells, and downregulating the expression of androgen receptor (AR) and oestrogen receptor α (ERα). An in vitro study showed that DHA inhibited the growth of the human prostate stromal cell line WPMY-1 and the epithelial cell line RWPE-1 in a dose- and time-dependent manner. In both cell lines, the DHA arrested the cell cycle in the G2/M phase. In addition, DHA also reduced the expression of ERα and AR in the WPMY-1 and RWPE-1 cells. These results indicate that DHA inhibits the multiplication of prostate stromal and epithelial cells through a mechanism that may involve cell cycle arrest and the downregulation of ERα and AR expression. - Highlights: • Seal oil prevents oestradiol/testosterone (E2/T)-induced BPH in castrated rats. • Seal oil downregulates the expression of oestrogen receptor α(ERα) and androgen receptor (AR) in rat BPH tissues. • DHA inhibits the growth of human prostate stromal and epithelial cells in vitro. • DHA arrests human prostate stromal and epithelial cells in the G2/M phase and downregulates the expression of cyclin B1. • DHA inhibits the expression of ERα and AR in human prostate stromal and epithelial cells.},
doi = {10.1016/J.YEXCR.2015.03.026},
journal = {Experimental Cell Research},
number = 2,
volume = 345,
place = {United States},
year = {Fri Jul 15 00:00:00 EDT 2016},
month = {Fri Jul 15 00:00:00 EDT 2016}
}