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Title: Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation

Abstract

Modification of the bone marrow microenvironment is considered as a promising strategy to control leukemic cell proliferation, diseases progression and relapse after treatment. However, due to the diversity and complexity of the cellular and molecular compartments in the leukemic microenvironment, it is extremely difficult to dissect the role of each individual molecule or cell type in vivo. Here we established an in vitro system to dissect the role of lipopolysaccharide (LPS), stromal cells and endothelial cells in the growth of mouse myeloid tumor cells and B-lymphoma cells. We found that either LPS or bone marrow stromal cells as a feeder layer in culture is required for the proliferation of myeloid tumor cells. Surprisingly, the growth of myeloid leukemic cells on stromal cells is strongly inhibited when coupled with LPS in culture. This opposing effect of LPS, a complete switch from pro-proliferation to antitumor growth is due, at least in part, to the rapidly increased production of interleukin 12, Fas ligand and tissue inhibitor of metalloproteinases-2 from stromal cells stimulated by LPS. These results demonstrate that LPS can either facilitate or attenuate tumor cell proliferation, thus changing the disease course of myeloid leukemias through its direct effect or modulation of themore » tumor microenvironment. - Highlights: • LPS alone in culture is required for the proliferation of murine myeloid tumor cells. • Bone marrow stromal cells as a feeder layer is also required for the proliferation of myeloid tumor cells. • However, the growth of myeloid tumor cells is inhibited when LPS and stromal cells are both available in culture. • Thus LPS can either facilitate or attenuate tumor growth through its direct effect or modulation of tumor microenvironment.« less

Authors:
 [1];  [2];  [1]; ; ; ; ; ; ;  [3];  [4];  [1];  [2];  [2];  [2];  [2]
  1. Department of Pediatrics, Jingjiang People's Hospital, Yangzhou University, Jingjiang 214500 (China)
  2. (China)
  3. Noncoding RNA Center, Yangzhou University, Yangzhou 225001 (China)
  4. Department of Hematology, Yangzhou University School of Clinical Medicine, Yangzhou 225001 (China)
Publication Date:
OSTI Identifier:
22648583
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 344; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; CELL PROLIFERATION; COMPARTMENTS; CONTROL; IN VITRO; IN VIVO; LIGANDS; LIPOPOLYSACCHARIDES; LYMPHOKINES; LYMPHOMAS; MICE; MODIFICATIONS; MODULATION; MOLECULES; MYELOID LEUKEMIA; PLANT GROWTH; PLANT TISSUES; SKELETON; SWITCHES; TUMOR CELLS

Citation Formats

Yu, Lingling, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Zhao, Yingmin, Gu, Xin, Wang, Jijun, Pang, Lei, Zhang, Yanqing, Li, Yaoyao, Jia, Xiaoqin, Wang, Xin, Gu, Jian, Yu, Duonan, E-mail: duonan@yahoo.com, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou 225001, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225001, and Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225001. Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2016.03.006.
Yu, Lingling, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Zhao, Yingmin, Gu, Xin, Wang, Jijun, Pang, Lei, Zhang, Yanqing, Li, Yaoyao, Jia, Xiaoqin, Wang, Xin, Gu, Jian, Yu, Duonan, E-mail: duonan@yahoo.com, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou 225001, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225001, & Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225001. Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation. United States. doi:10.1016/J.YEXCR.2016.03.006.
Yu, Lingling, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Zhao, Yingmin, Gu, Xin, Wang, Jijun, Pang, Lei, Zhang, Yanqing, Li, Yaoyao, Jia, Xiaoqin, Wang, Xin, Gu, Jian, Yu, Duonan, E-mail: duonan@yahoo.com, Noncoding RNA Center, Yangzhou University, Yangzhou 225001, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou 225001, Institute of Comparative Medicine, Yangzhou University, Yangzhou 225001, and Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225001. Fri . "Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation". United States. doi:10.1016/J.YEXCR.2016.03.006.
@article{osti_22648583,
title = {Dual effect of LPS on murine myeloid leukemia cells: Pro-proliferation and anti-proliferation},
author = {Yu, Lingling and Noncoding RNA Center, Yangzhou University, Yangzhou 225001 and Zhao, Yingmin and Gu, Xin and Wang, Jijun and Pang, Lei and Zhang, Yanqing and Li, Yaoyao and Jia, Xiaoqin and Wang, Xin and Gu, Jian and Yu, Duonan, E-mail: duonan@yahoo.com and Noncoding RNA Center, Yangzhou University, Yangzhou 225001 and Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou 225001 and Institute of Comparative Medicine, Yangzhou University, Yangzhou 225001 and Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou 225001},
abstractNote = {Modification of the bone marrow microenvironment is considered as a promising strategy to control leukemic cell proliferation, diseases progression and relapse after treatment. However, due to the diversity and complexity of the cellular and molecular compartments in the leukemic microenvironment, it is extremely difficult to dissect the role of each individual molecule or cell type in vivo. Here we established an in vitro system to dissect the role of lipopolysaccharide (LPS), stromal cells and endothelial cells in the growth of mouse myeloid tumor cells and B-lymphoma cells. We found that either LPS or bone marrow stromal cells as a feeder layer in culture is required for the proliferation of myeloid tumor cells. Surprisingly, the growth of myeloid leukemic cells on stromal cells is strongly inhibited when coupled with LPS in culture. This opposing effect of LPS, a complete switch from pro-proliferation to antitumor growth is due, at least in part, to the rapidly increased production of interleukin 12, Fas ligand and tissue inhibitor of metalloproteinases-2 from stromal cells stimulated by LPS. These results demonstrate that LPS can either facilitate or attenuate tumor cell proliferation, thus changing the disease course of myeloid leukemias through its direct effect or modulation of the tumor microenvironment. - Highlights: • LPS alone in culture is required for the proliferation of murine myeloid tumor cells. • Bone marrow stromal cells as a feeder layer is also required for the proliferation of myeloid tumor cells. • However, the growth of myeloid tumor cells is inhibited when LPS and stromal cells are both available in culture. • Thus LPS can either facilitate or attenuate tumor growth through its direct effect or modulation of tumor microenvironment.},
doi = {10.1016/J.YEXCR.2016.03.006},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 344,
place = {United States},
year = {2016},
month = {6}
}