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Title: The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking

Abstract

Cells adjust their adhesive and cytoskeletal organizations according to changes in the biochemical and physical nature of their surroundings. In return, by adhering and generating forces on the extracellular matrix (ECM) cells organize their microenvironment. Integrin-dependent focal adhesions (FAs) are the converging zones integrating biochemical and biomechanical signals arising from the ECM and the actin cytoskeleton. Thus, integrin-mediated adhesion and mechanotransduction, the conversion of mechanical forces into biochemical signals, are involved in critical cellular functions such as migration, proliferation and differentiation, and their deregulation contributes to pathologies including cancer. A challenging problem is to decipher how stochastic protein movements and interactions lead to formation of dynamic architecture such as integrin-dependent adhesive structures. In this review, we will describe recent advances made possible by super-resolution microscopies and single molecule tracking approaches that provided new understanding on the organization and the dynamics of integrins and intracellular regulators at the nanoscale in living cells.

Authors:
;  [1]
  1. Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux (France)
Publication Date:
OSTI Identifier:
22648560
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 343; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; ADHESION; ARCHITECTURE; BIOMEDICAL RADIOGRAPHY; CONVERSION; CORRELATIONS; DEREGULATION; FLUORESCENCE; FUNCTIONS; MATERIALS RECOVERY; MICROSCOPY; MICROTUBULES; MIGRATION; MOLECULES; NANOSTRUCTURES; NEOPLASMS; PARTICLES; PATHOLOGY; PROLIFERATION; RESONANCE; REVIEWS; TOPOGRAPHY; ZONES

Citation Formats

Rossier, Olivier, Giannone, Grégory, and CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux. The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking. United States: N. p., 2016. Web. doi:10.1016/J.YEXCR.2015.11.004.
Rossier, Olivier, Giannone, Grégory, & CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux. The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking. United States. doi:10.1016/J.YEXCR.2015.11.004.
Rossier, Olivier, Giannone, Grégory, and CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux. Sun . "The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking". United States. doi:10.1016/J.YEXCR.2015.11.004.
@article{osti_22648560,
title = {The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking},
author = {Rossier, Olivier and Giannone, Grégory and CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux},
abstractNote = {Cells adjust their adhesive and cytoskeletal organizations according to changes in the biochemical and physical nature of their surroundings. In return, by adhering and generating forces on the extracellular matrix (ECM) cells organize their microenvironment. Integrin-dependent focal adhesions (FAs) are the converging zones integrating biochemical and biomechanical signals arising from the ECM and the actin cytoskeleton. Thus, integrin-mediated adhesion and mechanotransduction, the conversion of mechanical forces into biochemical signals, are involved in critical cellular functions such as migration, proliferation and differentiation, and their deregulation contributes to pathologies including cancer. A challenging problem is to decipher how stochastic protein movements and interactions lead to formation of dynamic architecture such as integrin-dependent adhesive structures. In this review, we will describe recent advances made possible by super-resolution microscopies and single molecule tracking approaches that provided new understanding on the organization and the dynamics of integrins and intracellular regulators at the nanoscale in living cells.},
doi = {10.1016/J.YEXCR.2015.11.004},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 1,
volume = 343,
place = {United States},
year = {2016},
month = {4}
}