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Title: Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site

Abstract

Crystals of porcine pancreatic carboxypeptidase B (CPB) were grown by the capillary counter-diffusion method in the presence of polyethylene glycol and zinc acetate. The three-dimensional structure of CPB was determined at 1.40 Å resolution using the X-ray diffraction data set collected from the crystals of the enzyme at the SPring 8 synchrotron facility and was refined to R{sub fact} = 17.19%, R{sub free} = 19.78%. The structure contains five zinc atoms, two of which are present in the active site of the enzyme, and an acetate ion. The arrangement of an additional zinc atom in the active site and the acetate ion is different from that reported by Yoshimoto et al.

Authors:
 [1];  [2];  [3];  [2]
  1. State Research Institute for Genetics and Selection of Industrial Microorganisms (Russian Federation)
  2. Russian Academy of Sciences, Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics” (Russian Federation)
  3. Shahid Beheshti University of Medical Sciences, Neuroscience Research Center (Iran, Islamic Republic of)
Publication Date:
OSTI Identifier:
22645197
Resource Type:
Journal Article
Resource Relation:
Journal Name: Crystallography Reports; Journal Volume: 62; Journal Issue: 2; Other Information: Copyright (c) 2017 Pleiades Publishing, Inc.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ACETATES; CARBOXYPEPTIDASES; PANCREAS; POLYETHYLENE GLYCOLS; POLYETHYLENES; SPRING-8 STORAGE RING; THREE-DIMENSIONAL LATTICES; X-RAY DIFFRACTION; ZINC

Citation Formats

Akparov, V. Kh., E-mail: valery@akparov.ru, Timofeev, V. I., E-mail: tostars@mail.ru, Maghsoudi, N. N., E-mail: maghsudi@yahoo.com, and Kuranova, I. P.. Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site. United States: N. p., 2017. Web. doi:10.1134/S106377451702002X.
Akparov, V. Kh., E-mail: valery@akparov.ru, Timofeev, V. I., E-mail: tostars@mail.ru, Maghsoudi, N. N., E-mail: maghsudi@yahoo.com, & Kuranova, I. P.. Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site. United States. doi:10.1134/S106377451702002X.
Akparov, V. Kh., E-mail: valery@akparov.ru, Timofeev, V. I., E-mail: tostars@mail.ru, Maghsoudi, N. N., E-mail: maghsudi@yahoo.com, and Kuranova, I. P.. Wed . "Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site". United States. doi:10.1134/S106377451702002X.
@article{osti_22645197,
title = {Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site},
author = {Akparov, V. Kh., E-mail: valery@akparov.ru and Timofeev, V. I., E-mail: tostars@mail.ru and Maghsoudi, N. N., E-mail: maghsudi@yahoo.com and Kuranova, I. P.},
abstractNote = {Crystals of porcine pancreatic carboxypeptidase B (CPB) were grown by the capillary counter-diffusion method in the presence of polyethylene glycol and zinc acetate. The three-dimensional structure of CPB was determined at 1.40 Å resolution using the X-ray diffraction data set collected from the crystals of the enzyme at the SPring 8 synchrotron facility and was refined to R{sub fact} = 17.19%, R{sub free} = 19.78%. The structure contains five zinc atoms, two of which are present in the active site of the enzyme, and an acetate ion. The arrangement of an additional zinc atom in the active site and the acetate ion is different from that reported by Yoshimoto et al.},
doi = {10.1134/S106377451702002X},
journal = {Crystallography Reports},
number = 2,
volume = 62,
place = {United States},
year = {Wed Mar 15 00:00:00 EDT 2017},
month = {Wed Mar 15 00:00:00 EDT 2017}
}
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