skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Radiation damage limits to XPCS studies of protein dynamics

Abstract

The limitations to x-ray photon correlation spectroscopy (XPCS) imposed by radiation damage have been evaluated for suspensions of alpha crystallin. We find that the threshold for radiation damage to the measured protein diffusion rate is significantly lower than the threshold for damage to the protein structure. We provide damage thresholds beyond which the measured diffusion coeffcients have been modified using both XPCS and dynamic light scattering (DLS).

Authors:
; ;  [1];  [2];  [3];  [4];  [5];  [4]; ;  [6];  [7]; ;  [8]
  1. Department of Physics, Northern Illinois University, DeKalb IL, 60115 (United States)
  2. Amity Center for Spintronic Materials, Amity University UP, Sector 125, NOIDA 201313 (India)
  3. School of Physics and Astronomy, Rochester Institute of Technology, Rochester, NY 14623-5603 (United States)
  4. Department of Computer Science, Northern Illinois University, DeKalb, IL 60115 (United States)
  5. (United States)
  6. Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60495 (United States)
  7. Department of Biology, Northern Illinois University, DeKalb, IL 60115 (United States)
  8. Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb IL, 60115 (United States)
Publication Date:
OSTI Identifier:
22608450
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1741; Journal Issue: 1; Conference: SRI2015: 12. international conference on synchrotron radiation instrumentation, New York, NY (United States), 6-10 Jul 2015; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CORRELATIONS; DAMAGE; DIFFUSION; LIGHT SCATTERING; PHOTONS; PHYSICAL RADIATION EFFECTS; PROTEIN STRUCTURE; PROTEINS; SUSPENSIONS; X RADIATION; X-RAY SPECTROSCOPY

Citation Formats

Vodnala, Preeti, E-mail: preeti.vodnala@gmail.com, Karunaratne, Nuwan, Lurio, Laurence, Bera, Sambhunath, Thurston, George M., Karonis, Nick, Mathematics and Computer Science, Argonne National Laboratory, Argonne, IL 60495, Winans, John, Sandy, Alec, Narayanan, Suresh, Yasui, Linda, Gaillard, Elizabeth, and Karumanchi, Kalyan. Radiation damage limits to XPCS studies of protein dynamics. United States: N. p., 2016. Web. doi:10.1063/1.4952946.
Vodnala, Preeti, E-mail: preeti.vodnala@gmail.com, Karunaratne, Nuwan, Lurio, Laurence, Bera, Sambhunath, Thurston, George M., Karonis, Nick, Mathematics and Computer Science, Argonne National Laboratory, Argonne, IL 60495, Winans, John, Sandy, Alec, Narayanan, Suresh, Yasui, Linda, Gaillard, Elizabeth, & Karumanchi, Kalyan. Radiation damage limits to XPCS studies of protein dynamics. United States. doi:10.1063/1.4952946.
Vodnala, Preeti, E-mail: preeti.vodnala@gmail.com, Karunaratne, Nuwan, Lurio, Laurence, Bera, Sambhunath, Thurston, George M., Karonis, Nick, Mathematics and Computer Science, Argonne National Laboratory, Argonne, IL 60495, Winans, John, Sandy, Alec, Narayanan, Suresh, Yasui, Linda, Gaillard, Elizabeth, and Karumanchi, Kalyan. 2016. "Radiation damage limits to XPCS studies of protein dynamics". United States. doi:10.1063/1.4952946.
@article{osti_22608450,
title = {Radiation damage limits to XPCS studies of protein dynamics},
author = {Vodnala, Preeti, E-mail: preeti.vodnala@gmail.com and Karunaratne, Nuwan and Lurio, Laurence and Bera, Sambhunath and Thurston, George M. and Karonis, Nick and Mathematics and Computer Science, Argonne National Laboratory, Argonne, IL 60495 and Winans, John and Sandy, Alec and Narayanan, Suresh and Yasui, Linda and Gaillard, Elizabeth and Karumanchi, Kalyan},
abstractNote = {The limitations to x-ray photon correlation spectroscopy (XPCS) imposed by radiation damage have been evaluated for suspensions of alpha crystallin. We find that the threshold for radiation damage to the measured protein diffusion rate is significantly lower than the threshold for damage to the protein structure. We provide damage thresholds beyond which the measured diffusion coeffcients have been modified using both XPCS and dynamic light scattering (DLS).},
doi = {10.1063/1.4952946},
journal = {AIP Conference Proceedings},
number = 1,
volume = 1741,
place = {United States},
year = 2016,
month = 7
}