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Title: Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique

Abstract

Bio-diagnostic assays of high complexity rely on nanoscaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. High throughput performance requires the simultaneous detection of various analytes combined with appropriate bioassay components. Nanoparticle induced sensitivity enhancement, and subsequent multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are fitting well these purposes. SEIRA constitutes an ideal platform to isolate the vibrational signatures of targeted bioassay and active molecules. The potential of several targeted biolabels, here fluorophore-labeled antibody conjugates, chemisorbed onto low-cost biocompatible gold nano-aggregates substrates have been explored for their use in assay platforms. Dried films were analyzed by synchrotron radiation based FTIR/SEIRA spectro-microscopy and the resulting complex hyperspectral datasets were submitted to automated statistical analysis, namely Principal Components Analysis (PCA). The relationships between molecular fingerprints were put in evidence to highlight their spectral discrimination capabilities. We demonstrate that robust spectral encoding via SEIRA fingerprints opens up new opportunities for fast, reliable and multiplexed high-end screening not only in biodiagnostics but also in vitro biochemical imaging.

Authors:
; ; ;  [1];  [2];  [3]
  1. Physikalisch-Technische Bundesanstalt, Abbestr. 2-12, 10587 Berlin (Germany)
  2. Elettra-Sincrotrone Trieste S.C.p.A., Strada Statale 14, Area Science Park, 34149 Trieste (Italy)
  3. BAM Bundesanstalt für Materialforschung und –prüfung, Richard-Willstätter-Str.10, 12489 Berlin (Germany)
Publication Date:
OSTI Identifier:
22608433
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1741; Journal Issue: 1; Conference: SRI2015: 12. international conference on synchrotron radiation instrumentation, New York, NY (United States), 6-10 Jul 2015; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; ANTIBODIES; CHEMISORPTION; DATASETS; DESIGN; DETECTION; FILMS; FOURIER TRANSFORMATION; GOLD; INFRARED SPECTRA; MICROSCOPY; MOLECULES; NANOPARTICLES; NANOSTRUCTURES; POLAR-CAP ABSORPTION; SCREENING; SENSITIVITY; SUBSTRATES; SURFACES; SYNCHROTRON RADIATION; SYNCHROTRONS

Citation Formats

Hornemann, Andrea, E-mail: andrea.hornemann@ptb.de, Hoehl, Arne, E-mail: arne.hoehl@ptb.de, Ulm, Gerhard, E-mail: gerhard.ulm@ptb.de, Beckhoff, Burkhard, E-mail: burkhard.beckhoff@ptb.de, Eichert, Diane, E-mail: diane.eichert@elettra.eu, and Flemig, Sabine, E-mail: sabine.flemig@bam.de. Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique. United States: N. p., 2016. Web. doi:10.1063/1.4952927.
Hornemann, Andrea, E-mail: andrea.hornemann@ptb.de, Hoehl, Arne, E-mail: arne.hoehl@ptb.de, Ulm, Gerhard, E-mail: gerhard.ulm@ptb.de, Beckhoff, Burkhard, E-mail: burkhard.beckhoff@ptb.de, Eichert, Diane, E-mail: diane.eichert@elettra.eu, & Flemig, Sabine, E-mail: sabine.flemig@bam.de. Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique. United States. doi:10.1063/1.4952927.
Hornemann, Andrea, E-mail: andrea.hornemann@ptb.de, Hoehl, Arne, E-mail: arne.hoehl@ptb.de, Ulm, Gerhard, E-mail: gerhard.ulm@ptb.de, Beckhoff, Burkhard, E-mail: burkhard.beckhoff@ptb.de, Eichert, Diane, E-mail: diane.eichert@elettra.eu, and Flemig, Sabine, E-mail: sabine.flemig@bam.de. Wed . "Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique". United States. doi:10.1063/1.4952927.
@article{osti_22608433,
title = {Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique},
author = {Hornemann, Andrea, E-mail: andrea.hornemann@ptb.de and Hoehl, Arne, E-mail: arne.hoehl@ptb.de and Ulm, Gerhard, E-mail: gerhard.ulm@ptb.de and Beckhoff, Burkhard, E-mail: burkhard.beckhoff@ptb.de and Eichert, Diane, E-mail: diane.eichert@elettra.eu and Flemig, Sabine, E-mail: sabine.flemig@bam.de},
abstractNote = {Bio-diagnostic assays of high complexity rely on nanoscaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. High throughput performance requires the simultaneous detection of various analytes combined with appropriate bioassay components. Nanoparticle induced sensitivity enhancement, and subsequent multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are fitting well these purposes. SEIRA constitutes an ideal platform to isolate the vibrational signatures of targeted bioassay and active molecules. The potential of several targeted biolabels, here fluorophore-labeled antibody conjugates, chemisorbed onto low-cost biocompatible gold nano-aggregates substrates have been explored for their use in assay platforms. Dried films were analyzed by synchrotron radiation based FTIR/SEIRA spectro-microscopy and the resulting complex hyperspectral datasets were submitted to automated statistical analysis, namely Principal Components Analysis (PCA). The relationships between molecular fingerprints were put in evidence to highlight their spectral discrimination capabilities. We demonstrate that robust spectral encoding via SEIRA fingerprints opens up new opportunities for fast, reliable and multiplexed high-end screening not only in biodiagnostics but also in vitro biochemical imaging.},
doi = {10.1063/1.4952927},
journal = {AIP Conference Proceedings},
number = 1,
volume = 1741,
place = {United States},
year = {Wed Jul 27 00:00:00 EDT 2016},
month = {Wed Jul 27 00:00:00 EDT 2016}
}