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Title: The characterization of exosome from blood plasma of patients with colorectal cancer

Abstract

Exosomes are extracellular membrane structures involved in many physiological and pathological processes including cancerogenesis and metastasis. The clarification of the criteria for exosome isolating and identifying is the purpose of this study. Exosome samples from the plasma of patients with colorectal cancer and healthy donors were examined using transmission electron microscopy and flow cytometry in accordance with the minimum requirements of “International Society for Extracellular Vesicles”. The choice of the method for isolation of exosomes from the blood plasma by ultrafiltration and ultracentrifugation allowed obtaining highly purified samples of exosomes, in which all the structural components were clearly seen. The results obtained with flow cytometry suggest that exosomes of blood plasma from patients with colorectal cancer can be produced by epithelial cells. Moreover, cells produce different types of exosomes, which correspond to different mechanisms in sorting macromolecules in the membrane of multivesicular bodies. Determination of significant differences in the expression of specific exosomal proteins from colorectal cancer patients compared to healthy donors suggests a high diagnostic potential significance of circulating exosomes.

Authors:
 [1];  [2];  [3];  [2]; ; ;  [1];  [4]
  1. Tomsk Cancer Research Institute, Kooperativny Street 5, Tomsk, 634009 (Russian Federation)
  2. (Russian Federation)
  3. Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk, 630090 (Russian Federation)
  4. Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050 (Russian Federation)
Publication Date:
OSTI Identifier:
22608294
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1760; Journal Issue: 1; Conference: PC'16: International conference on physics of cancer: Interdisciplinary problems and clinical applications 2016, Tomsk (Russian Federation), 22-25 Mar 2016; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD PLASMA; COMPARATIVE EVALUATIONS; ELECTRONS; MEMBRANES; METASTASES; NEOPLASMS; PATIENTS; PLASMA; TRANSMISSION; TRANSMISSION ELECTRON MICROSCOPY; ULTRACENTRIFUGATION; ULTRAFILTRATION

Citation Formats

Yunusova, N. V., E-mail: Bochkarevanv@oncology.tomsk.ru, Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050, Tamkovich, S. N., E-mail: s.tamk@niboch.nsc.ru, Novosibirsk State University, Pirogov Street 2, Novosibirsk, 630090, Stakheeva, M. N., E-mail: StakheyevaM@oncology.tomsk.ru, Afanas’ev, S. G., E-mail: Afanasievsg@oncology.tomsk.ru, Kondakova, I. V., E-mail: Kondakova@oncology.tomsk.ru, and Frolova, A. Y., E-mail: Frolovalenya@mail.ru. The characterization of exosome from blood plasma of patients with colorectal cancer. United States: N. p., 2016. Web. doi:10.1063/1.4960289.
Yunusova, N. V., E-mail: Bochkarevanv@oncology.tomsk.ru, Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050, Tamkovich, S. N., E-mail: s.tamk@niboch.nsc.ru, Novosibirsk State University, Pirogov Street 2, Novosibirsk, 630090, Stakheeva, M. N., E-mail: StakheyevaM@oncology.tomsk.ru, Afanas’ev, S. G., E-mail: Afanasievsg@oncology.tomsk.ru, Kondakova, I. V., E-mail: Kondakova@oncology.tomsk.ru, & Frolova, A. Y., E-mail: Frolovalenya@mail.ru. The characterization of exosome from blood plasma of patients with colorectal cancer. United States. doi:10.1063/1.4960289.
Yunusova, N. V., E-mail: Bochkarevanv@oncology.tomsk.ru, Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050, Tamkovich, S. N., E-mail: s.tamk@niboch.nsc.ru, Novosibirsk State University, Pirogov Street 2, Novosibirsk, 630090, Stakheeva, M. N., E-mail: StakheyevaM@oncology.tomsk.ru, Afanas’ev, S. G., E-mail: Afanasievsg@oncology.tomsk.ru, Kondakova, I. V., E-mail: Kondakova@oncology.tomsk.ru, and Frolova, A. Y., E-mail: Frolovalenya@mail.ru. Tue . "The characterization of exosome from blood plasma of patients with colorectal cancer". United States. doi:10.1063/1.4960289.
@article{osti_22608294,
title = {The characterization of exosome from blood plasma of patients with colorectal cancer},
author = {Yunusova, N. V., E-mail: Bochkarevanv@oncology.tomsk.ru and Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050 and Tamkovich, S. N., E-mail: s.tamk@niboch.nsc.ru and Novosibirsk State University, Pirogov Street 2, Novosibirsk, 630090 and Stakheeva, M. N., E-mail: StakheyevaM@oncology.tomsk.ru and Afanas’ev, S. G., E-mail: Afanasievsg@oncology.tomsk.ru and Kondakova, I. V., E-mail: Kondakova@oncology.tomsk.ru and Frolova, A. Y., E-mail: Frolovalenya@mail.ru},
abstractNote = {Exosomes are extracellular membrane structures involved in many physiological and pathological processes including cancerogenesis and metastasis. The clarification of the criteria for exosome isolating and identifying is the purpose of this study. Exosome samples from the plasma of patients with colorectal cancer and healthy donors were examined using transmission electron microscopy and flow cytometry in accordance with the minimum requirements of “International Society for Extracellular Vesicles”. The choice of the method for isolation of exosomes from the blood plasma by ultrafiltration and ultracentrifugation allowed obtaining highly purified samples of exosomes, in which all the structural components were clearly seen. The results obtained with flow cytometry suggest that exosomes of blood plasma from patients with colorectal cancer can be produced by epithelial cells. Moreover, cells produce different types of exosomes, which correspond to different mechanisms in sorting macromolecules in the membrane of multivesicular bodies. Determination of significant differences in the expression of specific exosomal proteins from colorectal cancer patients compared to healthy donors suggests a high diagnostic potential significance of circulating exosomes.},
doi = {10.1063/1.4960289},
journal = {AIP Conference Proceedings},
number = 1,
volume = 1760,
place = {United States},
year = {Tue Aug 02 00:00:00 EDT 2016},
month = {Tue Aug 02 00:00:00 EDT 2016}
}