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Title: Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds

Abstract

In this study, the performances of different preparation methods of the scaffolds were analyzed for chondrocyte tissue engineering. Silk fibroin/collagen (SF/C) was fabricated using a vacuum freeze-dried technique and by 3D printing. The porosity, water absorption expansion rates, mechanical properties, and pore sizes of the resulting materials were evaluated. The proliferation and metabolism of the cells was detected at different time points using an MTT assay. Cell morphologies and distributions were observed by histological analysis and scanning electron microscopy (SEM). The porosity, water absorption expansion rate, and Young’s modulus of the material obtained via 3D printing were significantly higher than those obtained by the freeze-dried method, while the pore size did not differ significantly between the two methods. MTT assay results showed that the metabolism of cells seeded on the 3D printed scaffolds was more viable than the metabolism on the freeze-dried material. H&E staining of the scaffolds revealed that the number of cells in the 3D printed scaffold was higher in comparison to a similar measurement on the freeze-dried material. Consequently, stem cells grew well inside the 3D printed scaffolds, as measured by SEM, while the internal structure of the freeze-dried scaffold was disordered. Compared with the freeze-dried technique,more » the 3D printed scaffold exhibited better overall performance and was more suitable for cartilage tissue engineering. - Highlights: • Silk fibroin/collagen was fabricated using 3D printing. • Physical characterization and Cell compatibility were compared. • 3D printed scaffold exhibited better overall performance.« less

Authors:
 [1];  [2];  [1];  [1];  [3]
  1. Tianjin First Center Hospital, No. 24 Fukang Road, Tianjin, TJ 300192 (China)
  2. Institute of Medical Equipment, Academy of Military and Medical Sciences, No. 106, Wandong Street, Hedong District, Tianjin 300000 (China)
  3. Tianjin Medical University General Hospital, No. 154 Anshan Road, Tianjin, TJ 300052 (China)
Publication Date:
OSTI Identifier:
22606201
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSORPTION; CARTILAGE; CELL PROLIFERATION; COLLAGEN; ELECTRON SCANNING; LYOPHILIZATION; METABOLISM; MORPHOLOGY; PERFORMANCE; POROSITY; SCANNING ELECTRON MICROSCOPY; STEM CELLS

Citation Formats

Sun, Kai, Li, Ruixin, Jiang, Wenxue, E-mail: jiangortholivea@sina.cn, Sun, Yufu, and Li, Hui. Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.07.050.
Sun, Kai, Li, Ruixin, Jiang, Wenxue, E-mail: jiangortholivea@sina.cn, Sun, Yufu, & Li, Hui. Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds. United States. doi:10.1016/J.BBRC.2016.07.050.
Sun, Kai, Li, Ruixin, Jiang, Wenxue, E-mail: jiangortholivea@sina.cn, Sun, Yufu, and Li, Hui. Fri . "Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds". United States. doi:10.1016/J.BBRC.2016.07.050.
@article{osti_22606201,
title = {Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds},
author = {Sun, Kai and Li, Ruixin and Jiang, Wenxue, E-mail: jiangortholivea@sina.cn and Sun, Yufu and Li, Hui},
abstractNote = {In this study, the performances of different preparation methods of the scaffolds were analyzed for chondrocyte tissue engineering. Silk fibroin/collagen (SF/C) was fabricated using a vacuum freeze-dried technique and by 3D printing. The porosity, water absorption expansion rates, mechanical properties, and pore sizes of the resulting materials were evaluated. The proliferation and metabolism of the cells was detected at different time points using an MTT assay. Cell morphologies and distributions were observed by histological analysis and scanning electron microscopy (SEM). The porosity, water absorption expansion rate, and Young’s modulus of the material obtained via 3D printing were significantly higher than those obtained by the freeze-dried method, while the pore size did not differ significantly between the two methods. MTT assay results showed that the metabolism of cells seeded on the 3D printed scaffolds was more viable than the metabolism on the freeze-dried material. H&E staining of the scaffolds revealed that the number of cells in the 3D printed scaffold was higher in comparison to a similar measurement on the freeze-dried material. Consequently, stem cells grew well inside the 3D printed scaffolds, as measured by SEM, while the internal structure of the freeze-dried scaffold was disordered. Compared with the freeze-dried technique, the 3D printed scaffold exhibited better overall performance and was more suitable for cartilage tissue engineering. - Highlights: • Silk fibroin/collagen was fabricated using 3D printing. • Physical characterization and Cell compatibility were compared. • 3D printed scaffold exhibited better overall performance.},
doi = {10.1016/J.BBRC.2016.07.050},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 477,
place = {United States},
year = {Fri Sep 02 00:00:00 EDT 2016},
month = {Fri Sep 02 00:00:00 EDT 2016}
}