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Title: Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes

Abstract

Biochanin-A, a phytoestrogen derived from herbal plants, protected from the IL-1β-induced loss of proteoglycans through the suppression of matrix degrading enzymes such as matrix metalloproteinase (MMP)-13, MMP-3, MMP-1, and ADAMTS-5 in primary rat chondrocytes and the knee articular cartilage. It also suppressed the expression of IL-1β-induced catabolic factors such as nitric oxide synthase 2, cyclooxygenase-2, prostaglandin E{sub 2}, and inflammatory cytokines. Furthermore, biochanin-A suppressed the IL-1β-induced phosphorylation of NFκB, and inhibited its nuclear translocation in primary rat chondrocytes. These results indicate that biochanin-A antagonizes the IL-1β-induced catabolic effects through its anti-inflammatory activity that involves the modulation of NFκB signaling. - Highlights: • Biochanin-A is a phytoestrogen derived from medicinal plants. • It suppressed the IL-1β-induced matrix degrading enzymes and catabolic factors. • It inhibited IL-1β-induced proteoglycan loss in chondrocytes and cartilage tissues. • Its anti-catabolic effects were mediated by modulation of NFκB signaling. • It may be used as a potential anti-catabolic biomaterial for osteoarthritis.

Authors:
 [1]; ;  [2];  [1];  [3];  [4];  [5]; ;  [6];  [1];  [7];  [8];  [6]
  1. Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452 (Korea, Republic of)
  2. Department of Dental Bioengineering, Chosun University, Gwangju, 61452 (Korea, Republic of)
  3. Department of Periodontology, Chosun University, Gwangju, 61452 (Korea, Republic of)
  4. Department of Prosthodontics, Chosun University, Gwangju, 61452 (Korea, Republic of)
  5. Department of Oral and Maxillofacial Radiology, Chosun University, Gwangju, 61452 (Korea, Republic of)
  6. Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, 61452 (Korea, Republic of)
  7. Korea Basic Science Institute, Gwangju Center, Chonnam National University, Gwangju, 61186 (Korea, Republic of)
  8. Department of Biochemistry, Rush University Medical Center, Chicago, IL, 60612 (United States)
Publication Date:
OSTI Identifier:
22606186
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MATERIALS; BONE JOINTS; CARTILAGE; ENZYMES; INFLAMMATION; INHIBITION; LYMPHOKINES; MEDICINAL PLANTS; MODULATION; NITRIC OXIDE; PHOSPHORYLATION; PROSTAGLANDINS; RATS; TRANSLOCATION

Citation Formats

Oh, Ji-Su, Cho, In-A, Kang, Kyeong-Rok, You, Jae-Seek, Yu, Sang-Joun, Lee, Gyeong-Je, Seo, Yo-Seob, Kim, Chun Sung, Kim, Do Kyung, Kim, Su-Gwan, Seo, Young-Woo, Im, Hee-Jeong, and Kim, Jae-Sung, E-mail: js_kim@chosun.ac.kr. Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.126.
Oh, Ji-Su, Cho, In-A, Kang, Kyeong-Rok, You, Jae-Seek, Yu, Sang-Joun, Lee, Gyeong-Je, Seo, Yo-Seob, Kim, Chun Sung, Kim, Do Kyung, Kim, Su-Gwan, Seo, Young-Woo, Im, Hee-Jeong, & Kim, Jae-Sung, E-mail: js_kim@chosun.ac.kr. Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes. United States. doi:10.1016/J.BBRC.2016.06.126.
Oh, Ji-Su, Cho, In-A, Kang, Kyeong-Rok, You, Jae-Seek, Yu, Sang-Joun, Lee, Gyeong-Je, Seo, Yo-Seob, Kim, Chun Sung, Kim, Do Kyung, Kim, Su-Gwan, Seo, Young-Woo, Im, Hee-Jeong, and Kim, Jae-Sung, E-mail: js_kim@chosun.ac.kr. Fri . "Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes". United States. doi:10.1016/J.BBRC.2016.06.126.
@article{osti_22606186,
title = {Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes},
author = {Oh, Ji-Su and Cho, In-A and Kang, Kyeong-Rok and You, Jae-Seek and Yu, Sang-Joun and Lee, Gyeong-Je and Seo, Yo-Seob and Kim, Chun Sung and Kim, Do Kyung and Kim, Su-Gwan and Seo, Young-Woo and Im, Hee-Jeong and Kim, Jae-Sung, E-mail: js_kim@chosun.ac.kr},
abstractNote = {Biochanin-A, a phytoestrogen derived from herbal plants, protected from the IL-1β-induced loss of proteoglycans through the suppression of matrix degrading enzymes such as matrix metalloproteinase (MMP)-13, MMP-3, MMP-1, and ADAMTS-5 in primary rat chondrocytes and the knee articular cartilage. It also suppressed the expression of IL-1β-induced catabolic factors such as nitric oxide synthase 2, cyclooxygenase-2, prostaglandin E{sub 2}, and inflammatory cytokines. Furthermore, biochanin-A suppressed the IL-1β-induced phosphorylation of NFκB, and inhibited its nuclear translocation in primary rat chondrocytes. These results indicate that biochanin-A antagonizes the IL-1β-induced catabolic effects through its anti-inflammatory activity that involves the modulation of NFκB signaling. - Highlights: • Biochanin-A is a phytoestrogen derived from medicinal plants. • It suppressed the IL-1β-induced matrix degrading enzymes and catabolic factors. • It inhibited IL-1β-induced proteoglycan loss in chondrocytes and cartilage tissues. • Its anti-catabolic effects were mediated by modulation of NFκB signaling. • It may be used as a potential anti-catabolic biomaterial for osteoarthritis.},
doi = {10.1016/J.BBRC.2016.06.126},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 477,
place = {United States},
year = {Fri Sep 02 00:00:00 EDT 2016},
month = {Fri Sep 02 00:00:00 EDT 2016}
}