Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes
- Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Department of Dental Bioengineering, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Department of Periodontology, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Department of Prosthodontics, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Department of Oral and Maxillofacial Radiology, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, 61452 (Korea, Republic of)
- Korea Basic Science Institute, Gwangju Center, Chonnam National University, Gwangju, 61186 (Korea, Republic of)
- Department of Biochemistry, Rush University Medical Center, Chicago, IL, 60612 (United States)
Biochanin-A, a phytoestrogen derived from herbal plants, protected from the IL-1β-induced loss of proteoglycans through the suppression of matrix degrading enzymes such as matrix metalloproteinase (MMP)-13, MMP-3, MMP-1, and ADAMTS-5 in primary rat chondrocytes and the knee articular cartilage. It also suppressed the expression of IL-1β-induced catabolic factors such as nitric oxide synthase 2, cyclooxygenase-2, prostaglandin E{sub 2}, and inflammatory cytokines. Furthermore, biochanin-A suppressed the IL-1β-induced phosphorylation of NFκB, and inhibited its nuclear translocation in primary rat chondrocytes. These results indicate that biochanin-A antagonizes the IL-1β-induced catabolic effects through its anti-inflammatory activity that involves the modulation of NFκB signaling. - Highlights: • Biochanin-A is a phytoestrogen derived from medicinal plants. • It suppressed the IL-1β-induced matrix degrading enzymes and catabolic factors. • It inhibited IL-1β-induced proteoglycan loss in chondrocytes and cartilage tissues. • Its anti-catabolic effects were mediated by modulation of NFκB signaling. • It may be used as a potential anti-catabolic biomaterial for osteoarthritis.
- OSTI ID:
- 22606186
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 477, Issue 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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