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Title: Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan

Abstract

Hypoxia-induced apoptosis-related mechanisms involved in the brain damage following cerebral ischemia injury. A subset of the small noncoding microRNA (miRNAs) is regulated by tissue oxygen levels, and miR-24 was found to be activated by hypoxic conditions. However, the roles of miR-24 and its target gene in neuron are not well understood. Here, we validated miRNA-24 is down-regulated in patients with cerebral infarction. Hypoxia suppressed the expression of miR-24, but increased the expression of neurocan in both mRNA and protein levels in SH-SY5Y cells. MiR-24 mimics reduced the expression of neurocan, suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. By luciferase reporter assay, neurocan is validated a direct target gene of miR-24. Furthermore, knockdown of neurocan suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. Taken together, miR-24 overexpression or silencing of neurocan shows an antihypoxic effect in SH-SY5Y cells. Therefore, miR-24 and neurocan play critical roles in neuron cell apoptosis and are potential therapeutic targets for ischemic brain disease. - Highlights: • miR-24 and neurocan play critical roles in neuron cell apoptosis. • miR-24 and neurocan are potential therapeutic targets for ischemic brain disease. • Antihypoxic effectmore » of miR-24 and neurocan in SH-SY5Y cells.« less

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22606185
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ANOXIA; APOPTOSIS; BRAIN; CELL CYCLE; CELL PROLIFERATION; INJURIES; ISCHEMIA; LUCIFERASE; MESSENGER-RNA; NERVE CELLS; PATIENTS

Citation Formats

Sun, Xingyuan, E-mail: sunxingyuan@sina.com, Ren, Zhanjun, Pan, Yunzhi, and Zhang, Chenxin. Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.121.
Sun, Xingyuan, E-mail: sunxingyuan@sina.com, Ren, Zhanjun, Pan, Yunzhi, & Zhang, Chenxin. Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan. United States. doi:10.1016/J.BBRC.2016.06.121.
Sun, Xingyuan, E-mail: sunxingyuan@sina.com, Ren, Zhanjun, Pan, Yunzhi, and Zhang, Chenxin. 2016. "Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan". United States. doi:10.1016/J.BBRC.2016.06.121.
@article{osti_22606185,
title = {Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan},
author = {Sun, Xingyuan, E-mail: sunxingyuan@sina.com and Ren, Zhanjun and Pan, Yunzhi and Zhang, Chenxin},
abstractNote = {Hypoxia-induced apoptosis-related mechanisms involved in the brain damage following cerebral ischemia injury. A subset of the small noncoding microRNA (miRNAs) is regulated by tissue oxygen levels, and miR-24 was found to be activated by hypoxic conditions. However, the roles of miR-24 and its target gene in neuron are not well understood. Here, we validated miRNA-24 is down-regulated in patients with cerebral infarction. Hypoxia suppressed the expression of miR-24, but increased the expression of neurocan in both mRNA and protein levels in SH-SY5Y cells. MiR-24 mimics reduced the expression of neurocan, suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. By luciferase reporter assay, neurocan is validated a direct target gene of miR-24. Furthermore, knockdown of neurocan suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. Taken together, miR-24 overexpression or silencing of neurocan shows an antihypoxic effect in SH-SY5Y cells. Therefore, miR-24 and neurocan play critical roles in neuron cell apoptosis and are potential therapeutic targets for ischemic brain disease. - Highlights: • miR-24 and neurocan play critical roles in neuron cell apoptosis. • miR-24 and neurocan are potential therapeutic targets for ischemic brain disease. • Antihypoxic effect of miR-24 and neurocan in SH-SY5Y cells.},
doi = {10.1016/J.BBRC.2016.06.121},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 477,
place = {United States},
year = 2016,
month = 9
}
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