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Title: TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria

Abstract

Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRβ1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRβ3*, β4*, β5* alleles. Complete PPII{sub L} 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRβ1*PBR pockets 1 and 9, a gauche{sup −} rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche{sup +} orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory. - Highlights: • Stereo-electronic and topochemical rules associated with FPLLI immunological memory. • Presence of very high long-lasting antibody titres against Plasmodium falciparum Spz. • Protective memory induction associated with a binding capacity to HLA-DRβ1*. • gauche{sup −} rotamer orientation in p8 polar residue is related to is related to immunological memory.

Authors:
;  [1];  [2];  [3];  [1]; ;  [1];  [2];  [1];  [2]
  1. Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C. (Colombia)
  2. (Colombia)
  3. (UDCA), Bogotá (Colombia)
Publication Date:
OSTI Identifier:
22606183
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; IMMUNITY; MALARIA; MONKEYS; ORIENTATION; PEPTIDES; PLASMODIUM; PROTEIN STRUCTURE; RECEPTORS; RESIDUES

Citation Formats

Alba, Martha P., Suarez, Carlos F., Universidad del Rosario, Bogotá D. C., Universidad de Ciencias Aplicadas y Ambientales, Varela, Yahson, Patarroyo, Manuel A., Bermudez, Adriana, Universidad del Rosario, Bogotá D. C., Patarroyo, Manuel E., E-mail: mepatarr@gmail.com, and Universidad Nacional de Colombia, Bogotá D. C. TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.115.
Alba, Martha P., Suarez, Carlos F., Universidad del Rosario, Bogotá D. C., Universidad de Ciencias Aplicadas y Ambientales, Varela, Yahson, Patarroyo, Manuel A., Bermudez, Adriana, Universidad del Rosario, Bogotá D. C., Patarroyo, Manuel E., E-mail: mepatarr@gmail.com, & Universidad Nacional de Colombia, Bogotá D. C. TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria. United States. doi:10.1016/J.BBRC.2016.06.115.
Alba, Martha P., Suarez, Carlos F., Universidad del Rosario, Bogotá D. C., Universidad de Ciencias Aplicadas y Ambientales, Varela, Yahson, Patarroyo, Manuel A., Bermudez, Adriana, Universidad del Rosario, Bogotá D. C., Patarroyo, Manuel E., E-mail: mepatarr@gmail.com, and Universidad Nacional de Colombia, Bogotá D. C. 2016. "TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria". United States. doi:10.1016/J.BBRC.2016.06.115.
@article{osti_22606183,
title = {TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria},
author = {Alba, Martha P. and Suarez, Carlos F. and Universidad del Rosario, Bogotá D. C. and Universidad de Ciencias Aplicadas y Ambientales and Varela, Yahson and Patarroyo, Manuel A. and Bermudez, Adriana and Universidad del Rosario, Bogotá D. C. and Patarroyo, Manuel E., E-mail: mepatarr@gmail.com and Universidad Nacional de Colombia, Bogotá D. C.},
abstractNote = {Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRβ1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRβ3*, β4*, β5* alleles. Complete PPII{sub L} 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRβ1*PBR pockets 1 and 9, a gauche{sup −} rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche{sup +} orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory. - Highlights: • Stereo-electronic and topochemical rules associated with FPLLI immunological memory. • Presence of very high long-lasting antibody titres against Plasmodium falciparum Spz. • Protective memory induction associated with a binding capacity to HLA-DRβ1*. • gauche{sup −} rotamer orientation in p8 polar residue is related to is related to immunological memory.},
doi = {10.1016/J.BBRC.2016.06.115},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 477,
place = {United States},
year = 2016,
month = 9
}
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