skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis

Abstract

Arabidopsis thaliana GOLDEN2-LIKE (GLKs) transcription factors play important roles in regulation of photosynthesis-associated nuclear genes, as well as participate in chloroplast development. However, the involvement of GLKs in plants resistance to virus remains largely unknown. Here, the relationship between GLKs and Cucumber mosaic virus (CMV) stress response was investigated. Our results showed that the Arabidopsis glk1glk2 double-mutant was more susceptible to CMV infection and suffered more serious damages (such as higher oxidative damages, more compromised in PSII photochemistry and more reactive oxygen species accumulation) when compared with the wild-type plants. Interestingly, there was little difference between single mutant (glk1 or glk2) and wild-type plants in response to CMV infection, suggesting GLK1 and GLK2 might function redundant in virus resistance in Arabidopsis. Furthermore, the induction of antioxidant system and defense-associated genes expression in the double mutant were inhibited when compared with single mutant or wild-type plants after CMV infection. Further evidences showed that salicylic acid (SA) and jasmonic acid (JA) might be involved in GLKs-mediated virus resistance, as SA or JA level and synthesis-related genes transcription were impaired in glk1glk2 mutant. Taken together, our results indicated that GLKs played a positively role in virus resistance in Arabidopsis. - Highlights: • GLKsmore » play a positive role in CMV resistance in Arabidopsis. • Defective of GLKs suffered more ROS accumulation. • Arabidopsis lacking GLKs have damaged photosynthesis. • Arabidopsis lacking GLKs show low SA and JA accumulation.« less

Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22606180
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIOXIDANTS; ARABIDOPSIS; CHLOROPLASTS; COORDINATES; CUCUMBERS; GENES; MUTANTS; OXIDATION; PHOTOCHEMISTRY; PHOTOSYNTHESIS; SALICYLIC ACID; TOLERANCE; TRANSCRIPTION; TRANSCRIPTION FACTORS; VIRUSES

Citation Formats

Han, Xue-Ying, Li, Peng-Xu, Zou, Li-Juan, Tan, Wen-rong, Zheng, Ting, Zhang, Da-Wei, E-mail: yuanmiao1892@163.com, and Lin, Hong-Hui, E-mail: hhlin@scu.edu.cn. GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.110.
Han, Xue-Ying, Li, Peng-Xu, Zou, Li-Juan, Tan, Wen-rong, Zheng, Ting, Zhang, Da-Wei, E-mail: yuanmiao1892@163.com, & Lin, Hong-Hui, E-mail: hhlin@scu.edu.cn. GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis. United States. doi:10.1016/J.BBRC.2016.06.110.
Han, Xue-Ying, Li, Peng-Xu, Zou, Li-Juan, Tan, Wen-rong, Zheng, Ting, Zhang, Da-Wei, E-mail: yuanmiao1892@163.com, and Lin, Hong-Hui, E-mail: hhlin@scu.edu.cn. Fri . "GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis". United States. doi:10.1016/J.BBRC.2016.06.110.
@article{osti_22606180,
title = {GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis},
author = {Han, Xue-Ying and Li, Peng-Xu and Zou, Li-Juan and Tan, Wen-rong and Zheng, Ting and Zhang, Da-Wei, E-mail: yuanmiao1892@163.com and Lin, Hong-Hui, E-mail: hhlin@scu.edu.cn},
abstractNote = {Arabidopsis thaliana GOLDEN2-LIKE (GLKs) transcription factors play important roles in regulation of photosynthesis-associated nuclear genes, as well as participate in chloroplast development. However, the involvement of GLKs in plants resistance to virus remains largely unknown. Here, the relationship between GLKs and Cucumber mosaic virus (CMV) stress response was investigated. Our results showed that the Arabidopsis glk1glk2 double-mutant was more susceptible to CMV infection and suffered more serious damages (such as higher oxidative damages, more compromised in PSII photochemistry and more reactive oxygen species accumulation) when compared with the wild-type plants. Interestingly, there was little difference between single mutant (glk1 or glk2) and wild-type plants in response to CMV infection, suggesting GLK1 and GLK2 might function redundant in virus resistance in Arabidopsis. Furthermore, the induction of antioxidant system and defense-associated genes expression in the double mutant were inhibited when compared with single mutant or wild-type plants after CMV infection. Further evidences showed that salicylic acid (SA) and jasmonic acid (JA) might be involved in GLKs-mediated virus resistance, as SA or JA level and synthesis-related genes transcription were impaired in glk1glk2 mutant. Taken together, our results indicated that GLKs played a positively role in virus resistance in Arabidopsis. - Highlights: • GLKs play a positive role in CMV resistance in Arabidopsis. • Defective of GLKs suffered more ROS accumulation. • Arabidopsis lacking GLKs have damaged photosynthesis. • Arabidopsis lacking GLKs show low SA and JA accumulation.},
doi = {10.1016/J.BBRC.2016.06.110},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 477,
place = {United States},
year = {Fri Sep 02 00:00:00 EDT 2016},
month = {Fri Sep 02 00:00:00 EDT 2016}
}