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Title: 17β-estradiol regulates the differentiation of cementoblasts via Notch signaling cascade

Abstract

Estrogen has been well recognized as a key factor in the homeostasis of bone and periodontal tissue, but the way it regulates the activities of cementoblasts, the cell population maintaining cementum has not been fully understood. In this study, we examined the expression of estrogen receptor in OCCM-30 cells and the effect of 17β-estradiol (E2) on the proliferation and differentiation of OCCM-30 cells. We found that both estrogen receptor α and β were expressed in OCCM-30 cells. E2 exerted no significant influence on the proliferation of OCCM-30 cells, but inhibited the transcription and translation of BSP and Runx2 in the early phase of osteogenic induction except the BSP mRNA. Afterwards in the late phase of osteogenic induction, E2 enhanced the transcription and translation of BSP and Runx2 and promoted the calcium deposition. In addition, the expression level of Notch1, NICD and Hey1 mRNAs responded to exogenous E2 in a pattern similar to that of the osteoblastic markers. DAPT could attenuate the effect of E2 on the expression of osteoblastic markers. These findings indicated that E2 might regulate the differentiation of cementoblasts via Notch signaling. - Highlights: • 17β-estradiol showed no significant effect on the proliferation of cementoblasts. • 17β-estradiol promoted the osteoblastic differentiation ofmore » cementoblasts despite of an early transient inhibition. • Notch signaling was regulated by 17β-estradiol and was responsible for mediating the effect of E2 on cementoblasts. • Hey1 might display an opposite expression pattern to Notch signaling in certain circumstances.« less

Authors:
; ; ;  [1];  [2];  [1]
  1. Department of Orthodontics, The State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province (China)
  2. Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province (China)
Publication Date:
OSTI Identifier:
22606149
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 477; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; ESTRADIOL; HOMEOSTASIS; INHIBITION; MESSENGER-RNA; RECEPTORS; SKELETON; TRANSCRIPTION

Citation Formats

Liao, Jing, Zhou, Zeyuan, Huang, Li, Li, Yuyu, Li, Jingtao, and Zou, Shujuan, E-mail: drzsj@scu.edu.cn. 17β-estradiol regulates the differentiation of cementoblasts via Notch signaling cascade. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.028.
Liao, Jing, Zhou, Zeyuan, Huang, Li, Li, Yuyu, Li, Jingtao, & Zou, Shujuan, E-mail: drzsj@scu.edu.cn. 17β-estradiol regulates the differentiation of cementoblasts via Notch signaling cascade. United States. doi:10.1016/J.BBRC.2016.06.028.
Liao, Jing, Zhou, Zeyuan, Huang, Li, Li, Yuyu, Li, Jingtao, and Zou, Shujuan, E-mail: drzsj@scu.edu.cn. Fri . "17β-estradiol regulates the differentiation of cementoblasts via Notch signaling cascade". United States. doi:10.1016/J.BBRC.2016.06.028.
@article{osti_22606149,
title = {17β-estradiol regulates the differentiation of cementoblasts via Notch signaling cascade},
author = {Liao, Jing and Zhou, Zeyuan and Huang, Li and Li, Yuyu and Li, Jingtao and Zou, Shujuan, E-mail: drzsj@scu.edu.cn},
abstractNote = {Estrogen has been well recognized as a key factor in the homeostasis of bone and periodontal tissue, but the way it regulates the activities of cementoblasts, the cell population maintaining cementum has not been fully understood. In this study, we examined the expression of estrogen receptor in OCCM-30 cells and the effect of 17β-estradiol (E2) on the proliferation and differentiation of OCCM-30 cells. We found that both estrogen receptor α and β were expressed in OCCM-30 cells. E2 exerted no significant influence on the proliferation of OCCM-30 cells, but inhibited the transcription and translation of BSP and Runx2 in the early phase of osteogenic induction except the BSP mRNA. Afterwards in the late phase of osteogenic induction, E2 enhanced the transcription and translation of BSP and Runx2 and promoted the calcium deposition. In addition, the expression level of Notch1, NICD and Hey1 mRNAs responded to exogenous E2 in a pattern similar to that of the osteoblastic markers. DAPT could attenuate the effect of E2 on the expression of osteoblastic markers. These findings indicated that E2 might regulate the differentiation of cementoblasts via Notch signaling. - Highlights: • 17β-estradiol showed no significant effect on the proliferation of cementoblasts. • 17β-estradiol promoted the osteoblastic differentiation of cementoblasts despite of an early transient inhibition. • Notch signaling was regulated by 17β-estradiol and was responsible for mediating the effect of E2 on cementoblasts. • Hey1 might display an opposite expression pattern to Notch signaling in certain circumstances.},
doi = {10.1016/J.BBRC.2016.06.028},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 477,
place = {United States},
year = {Fri Aug 12 00:00:00 EDT 2016},
month = {Fri Aug 12 00:00:00 EDT 2016}
}