skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2

Abstract

miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy. - Highlights: • miR-411-5p is downregulated in breast cancer tissues and cell lines. • miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro. • GRB2more » is a direct target of miR-411-5p in breast cancer. • GRB2 is overexpressed in breast cancer and associates with disease outcome. • miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway.« less

Authors:
 [1];  [2];  [1];  [2]; ;  [1];  [1];  [2];  [3]; ; ;  [1];  [2];  [1];  [2];  [1];  [2]
  1. Department of Gastrointestinal Surgery, First Affiliated Hospital of Xiamen University, Xiamen 361003 (China)
  2. (China)
  3. State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102 (China)
Publication Date:
OSTI Identifier:
22606141
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; IN VITRO; MAMMARY GLANDS; METASTASES; NEOPLASMS

Citation Formats

Zhang, Yunda, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Xu, Guoxing, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Liu, Gang, Ye, Yongzhi, Zhang, Chuankai, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Fan, Chuannan, Wang, Haibin, Cai, Huali, Xiao, Rui, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Huang, Zhengjie, E-mail: huangzhengjie@xmu.edu.cn, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Luo, Qi, E-mail: luoqixmzsh@126.com, and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005. miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.06.006.
Zhang, Yunda, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Xu, Guoxing, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Liu, Gang, Ye, Yongzhi, Zhang, Chuankai, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Fan, Chuannan, Wang, Haibin, Cai, Huali, Xiao, Rui, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Huang, Zhengjie, E-mail: huangzhengjie@xmu.edu.cn, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Luo, Qi, E-mail: luoqixmzsh@126.com, & Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005. miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2. United States. doi:10.1016/J.BBRC.2016.06.006.
Zhang, Yunda, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Xu, Guoxing, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Liu, Gang, Ye, Yongzhi, Zhang, Chuankai, State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102, Fan, Chuannan, Wang, Haibin, Cai, Huali, Xiao, Rui, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Huang, Zhengjie, E-mail: huangzhengjie@xmu.edu.cn, Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005, Luo, Qi, E-mail: luoqixmzsh@126.com, and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005. Fri . "miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2". United States. doi:10.1016/J.BBRC.2016.06.006.
@article{osti_22606141,
title = {miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2},
author = {Zhang, Yunda and State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102 and Xu, Guoxing and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005 and Liu, Gang and Ye, Yongzhi and Zhang, Chuankai and State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen 361102 and Fan, Chuannan and Wang, Haibin and Cai, Huali and Xiao, Rui and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005 and Huang, Zhengjie, E-mail: huangzhengjie@xmu.edu.cn and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005 and Luo, Qi, E-mail: luoqixmzsh@126.com and Department of Gastrointestinal Surgery, First Clinical Medical College of Fujian Medical University, Fuzhou 350005},
abstractNote = {miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy. - Highlights: • miR-411-5p is downregulated in breast cancer tissues and cell lines. • miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro. • GRB2 is a direct target of miR-411-5p in breast cancer. • GRB2 is overexpressed in breast cancer and associates with disease outcome. • miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway.},
doi = {10.1016/J.BBRC.2016.06.006},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 476,
place = {United States},
year = {Fri Aug 05 00:00:00 EDT 2016},
month = {Fri Aug 05 00:00:00 EDT 2016}
}