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Title: In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay

Abstract

In mouse testes, spermatogonial stem cells (SSCs), a subpopulation of GFRα1 (GDNF family receptor-α1)-positive spermatogonia, are widely distributed along the convoluted seminiferous tubules. The proliferation and differentiation of the SSCs are regulated in part by local expression of GDNF (glial cell-derived neurotorphic factor), one of major niche factors for SSCs. However, the in vivo dynamics of the GDNF-stimulated GFRα1-positive spermatogonia remains unclear. Here, we developed a simple method for transplanting DiI-labeled and GDNF-soaked beads into the mouse testicular interstitium. By using this method, we examined the dynamics of GFRα1-positive spermatogonia in the tubular walls close to the transplanted GDNF-soaked beads. The bead-derived GDNF signals were able to induce the stratified aggregate formation of GFRα1-positive undifferentiated spermatogonia by day 3 post-transplantation. Each aggregate consisted of tightly compacted A{sub single} and marginal A{sub paired}–A{sub aligned} GFRα1-positive spermatogonia and was surrounded by A{sub aligned} GFRα1-negative spermatogonia at more advanced stages. These data not only provide in vivo evidence for the inductive roles of GDNF in forming a rapid aggregation of GFRα1-positive spermatogonia but also indicate the usefulness of this in vivo assay system of various growth factors for the stem/progenitor spermatogonia in mammalian spermatogenesis. - Highlights: • A novel bead transplantation assay was developed to examinemore » the in vivo effects of growth factors on spermatogonia. • A rapid aggregation of GFRα1-positive spermatogonia was induced by the transplanted GDNF-soaked beads. • Tightly-compacted A{sub single} and marginal A{sub paired}–A{sub aligned} spermatogonia were formed in each GFRα1-positive aggregate.« less

Authors:
; ; ;  [1]; ; ;  [2];  [3]; ;  [1];  [1]
  1. Department of Veterinary Anatomy, The University of Tokyo, Yayoi, Tokyo, 113-8657 (Japan)
  2. Department of Experimental Animal Model for Human Disease, Tokyo Medical and Dental University, Tokyo, 113-8510 (Japan)
  3. Center of Biomedical Research, Kyusyu University, Fukuoka, 812-8582 (Japan)
Publication Date:
OSTI Identifier:
22606138
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; GROWTH FACTORS; HISTONES; IN VIVO; MICE; RECEPTORS; SPERMATOGENESIS; SPERMATOGONIA; STEM CELLS; TESTES; TRANSPLANTS

Citation Formats

Uchida, Aya, Kishi, Kasane, Aiyama, Yoshimi, Miura, Kento, Takase, Hinako M., Suzuki, Hitomi, Kanai-Azuma, Masami, Iwamori, Tokuko, Kurohmaru, Masamichi, Tsunekawa, Naoki, and Kanai, Yoshiakira, E-mail: aykanai@mail.ecc.u-tokyo.ac.jp. In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.160.
Uchida, Aya, Kishi, Kasane, Aiyama, Yoshimi, Miura, Kento, Takase, Hinako M., Suzuki, Hitomi, Kanai-Azuma, Masami, Iwamori, Tokuko, Kurohmaru, Masamichi, Tsunekawa, Naoki, & Kanai, Yoshiakira, E-mail: aykanai@mail.ecc.u-tokyo.ac.jp. In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay. United States. doi:10.1016/J.BBRC.2016.05.160.
Uchida, Aya, Kishi, Kasane, Aiyama, Yoshimi, Miura, Kento, Takase, Hinako M., Suzuki, Hitomi, Kanai-Azuma, Masami, Iwamori, Tokuko, Kurohmaru, Masamichi, Tsunekawa, Naoki, and Kanai, Yoshiakira, E-mail: aykanai@mail.ecc.u-tokyo.ac.jp. Fri . "In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay". United States. doi:10.1016/J.BBRC.2016.05.160.
@article{osti_22606138,
title = {In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay},
author = {Uchida, Aya and Kishi, Kasane and Aiyama, Yoshimi and Miura, Kento and Takase, Hinako M. and Suzuki, Hitomi and Kanai-Azuma, Masami and Iwamori, Tokuko and Kurohmaru, Masamichi and Tsunekawa, Naoki and Kanai, Yoshiakira, E-mail: aykanai@mail.ecc.u-tokyo.ac.jp},
abstractNote = {In mouse testes, spermatogonial stem cells (SSCs), a subpopulation of GFRα1 (GDNF family receptor-α1)-positive spermatogonia, are widely distributed along the convoluted seminiferous tubules. The proliferation and differentiation of the SSCs are regulated in part by local expression of GDNF (glial cell-derived neurotorphic factor), one of major niche factors for SSCs. However, the in vivo dynamics of the GDNF-stimulated GFRα1-positive spermatogonia remains unclear. Here, we developed a simple method for transplanting DiI-labeled and GDNF-soaked beads into the mouse testicular interstitium. By using this method, we examined the dynamics of GFRα1-positive spermatogonia in the tubular walls close to the transplanted GDNF-soaked beads. The bead-derived GDNF signals were able to induce the stratified aggregate formation of GFRα1-positive undifferentiated spermatogonia by day 3 post-transplantation. Each aggregate consisted of tightly compacted A{sub single} and marginal A{sub paired}–A{sub aligned} GFRα1-positive spermatogonia and was surrounded by A{sub aligned} GFRα1-negative spermatogonia at more advanced stages. These data not only provide in vivo evidence for the inductive roles of GDNF in forming a rapid aggregation of GFRα1-positive spermatogonia but also indicate the usefulness of this in vivo assay system of various growth factors for the stem/progenitor spermatogonia in mammalian spermatogenesis. - Highlights: • A novel bead transplantation assay was developed to examine the in vivo effects of growth factors on spermatogonia. • A rapid aggregation of GFRα1-positive spermatogonia was induced by the transplanted GDNF-soaked beads. • Tightly-compacted A{sub single} and marginal A{sub paired}–A{sub aligned} spermatogonia were formed in each GFRα1-positive aggregate.},
doi = {10.1016/J.BBRC.2016.05.160},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 476,
place = {United States},
year = {Fri Aug 05 00:00:00 EDT 2016},
month = {Fri Aug 05 00:00:00 EDT 2016}
}