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Title: Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast

Abstract

Nucleosome dynamics facilitated by histone turnover is required for transcription as well as DNA replication and repair. Histone turnover is often associated with various histone modifications such as H3K56 acetylation (H3K56Ac), H3K36 methylation (H3K36me), and H4K20 methylation (H4K20me). In order to correlate histone modifications and transcription-dependent histone turnover, we performed genome wide analyses for euchromatic regions in G2/M-arrested fission yeast. The results show that transcription-dependent histone turnover at 5′ promoter and 3′ termination regions is directly correlated with the occurrence of H3K56Ac and H4K20 mono-methylation (H4K20me1) in actively transcribed genes. Furthermore, the increase of H3K56Ac and H4K20me1 and antisense RNA production was observed in the absence of the histone H3K36 methyltransferase Set2 and histone deacetylase complex (HDAC) that are involved in the suppression of histone turnover within the coding regions. These results together indicate that H4K20me1 as well as H3K56Ac are bona fide marks for transcription-dependent histone turnover in fission yeast.

Authors:
 [1];  [2];  [1];  [1]
  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141 (Korea, Republic of)
  2. Department of Chemistry and Biology, Korea Science Academy of KAIST, Busan, 614-822 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22606136
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETYLATION; DNA; DNA REPLICATION; FISSION; GENES; HISTONES; INHIBITION; LYSINE; METHYL TRANSFERASES; METHYLATION; NUCLEOSOMES; PROMOTERS; RNA; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION

Citation Formats

Yang, Hanna, Kwon, Chang Seob, Choi, Yoonjung, E-mail: jjungii@kaist.ac.kr, and Lee, Daeyoup, E-mail: daeyoup@kaist.ac.kr. Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.155.
Yang, Hanna, Kwon, Chang Seob, Choi, Yoonjung, E-mail: jjungii@kaist.ac.kr, & Lee, Daeyoup, E-mail: daeyoup@kaist.ac.kr. Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast. United States. doi:10.1016/J.BBRC.2016.05.155.
Yang, Hanna, Kwon, Chang Seob, Choi, Yoonjung, E-mail: jjungii@kaist.ac.kr, and Lee, Daeyoup, E-mail: daeyoup@kaist.ac.kr. Fri . "Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast". United States. doi:10.1016/J.BBRC.2016.05.155.
@article{osti_22606136,
title = {Both H4K20 mono-methylation and H3K56 acetylation mark transcription-dependent histone turnover in fission yeast},
author = {Yang, Hanna and Kwon, Chang Seob and Choi, Yoonjung, E-mail: jjungii@kaist.ac.kr and Lee, Daeyoup, E-mail: daeyoup@kaist.ac.kr},
abstractNote = {Nucleosome dynamics facilitated by histone turnover is required for transcription as well as DNA replication and repair. Histone turnover is often associated with various histone modifications such as H3K56 acetylation (H3K56Ac), H3K36 methylation (H3K36me), and H4K20 methylation (H4K20me). In order to correlate histone modifications and transcription-dependent histone turnover, we performed genome wide analyses for euchromatic regions in G2/M-arrested fission yeast. The results show that transcription-dependent histone turnover at 5′ promoter and 3′ termination regions is directly correlated with the occurrence of H3K56Ac and H4K20 mono-methylation (H4K20me1) in actively transcribed genes. Furthermore, the increase of H3K56Ac and H4K20me1 and antisense RNA production was observed in the absence of the histone H3K36 methyltransferase Set2 and histone deacetylase complex (HDAC) that are involved in the suppression of histone turnover within the coding regions. These results together indicate that H4K20me1 as well as H3K56Ac are bona fide marks for transcription-dependent histone turnover in fission yeast.},
doi = {10.1016/J.BBRC.2016.05.155},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 476,
place = {United States},
year = {Fri Aug 05 00:00:00 EDT 2016},
month = {Fri Aug 05 00:00:00 EDT 2016}
}