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Title: MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer

Abstract

The dysfunction of DNA damage repair (DDR) pathway contributes to tumorigenesis and drug-resistance in cancer. MUS81 is a member of the conserved xeroderma pigmentosum group F (XPF) family protein of endonucleases, which is important to the DDR pathway. However, the role of MUS81 in the development of ovarian cancer remains uncertain. To explore the expression of MUS81 and its association to serous ovarian cancer (SOC), 43 biopsies of SOC patients were detected by qRT-PCR, and 29 specimens were further performed by immunohistochemistry analysis. Here, we observed that MUS81 was over-expressed in SOC tissues at both transcript and protein levels, and the expression level of MUS81 protein in ovarian cancer cell lines was also higher than that in human normal ovarian surface epithelial cell line (HOSEpiC). We also found that down-regulation of MUS81 expression in ovarian cancer cells inhibited cell proliferation and colony formation ability, and influenced cell cycle progression. Moreover, inhibition of MUS81 expression induced cellular senescence and enhanced the antitumor effect of cisplatin. Down-regulation of MUS81 expression could suppress the growth and development of SOC. These results indicate that MUS81 might play important roles in the progression of SOC and influence the antitumor effect of cisplatin. - Highlights: •more » MUS81 was overexpression in serous ovarian cancer (SOC). • Meanwhile down-regulation of inhibited cell proliferation and influenced cell cycle progression. • Inhibition of MUS81 induced cell cellular senescence and enhanced the antitumor effect of cisplatin. • Down-regulation of MUS81 expression could suppress the growth and development of SOC.« less

Authors:
;  [1];  [2];  [3]; ; ; ;  [1];  [2];  [1];  [2]
  1. Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai (China)
  2. (China)
  3. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai (China)
Publication Date:
OSTI Identifier:
22606134
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BIOPSY; CELL CYCLE; CELL PROLIFERATION; COLONY FORMATION; CONGENITAL DISEASES; DNA DAMAGES; ENDONUCLEASES; HEREDITARY DISEASES; INHIBITION; NEOPLASMS; OVARIES; POLYMERASE CHAIN REACTION; SKIN DISEASES

Citation Formats

Xie, Suhong, Zheng, Hui, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Wen, Xuemei, Sun, Jiajun, Wang, Yanchun, Gao, Xiang, Guo, Lin, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Lu, Renquan, E-mail: lurenquan@126.com, and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai. MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.152.
Xie, Suhong, Zheng, Hui, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Wen, Xuemei, Sun, Jiajun, Wang, Yanchun, Gao, Xiang, Guo, Lin, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Lu, Renquan, E-mail: lurenquan@126.com, & Department of Oncology, Shanghai Medical College, Fudan University, Shanghai. MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer. United States. doi:10.1016/J.BBRC.2016.05.152.
Xie, Suhong, Zheng, Hui, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Wen, Xuemei, Sun, Jiajun, Wang, Yanchun, Gao, Xiang, Guo, Lin, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, Lu, Renquan, E-mail: lurenquan@126.com, and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai. 2016. "MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer". United States. doi:10.1016/J.BBRC.2016.05.152.
@article{osti_22606134,
title = {MUS81 is associated with cell proliferation and cisplatin sensitivity in serous ovarian cancer},
author = {Xie, Suhong and Zheng, Hui and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai and Wen, Xuemei and Sun, Jiajun and Wang, Yanchun and Gao, Xiang and Guo, Lin and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai and Lu, Renquan, E-mail: lurenquan@126.com and Department of Oncology, Shanghai Medical College, Fudan University, Shanghai},
abstractNote = {The dysfunction of DNA damage repair (DDR) pathway contributes to tumorigenesis and drug-resistance in cancer. MUS81 is a member of the conserved xeroderma pigmentosum group F (XPF) family protein of endonucleases, which is important to the DDR pathway. However, the role of MUS81 in the development of ovarian cancer remains uncertain. To explore the expression of MUS81 and its association to serous ovarian cancer (SOC), 43 biopsies of SOC patients were detected by qRT-PCR, and 29 specimens were further performed by immunohistochemistry analysis. Here, we observed that MUS81 was over-expressed in SOC tissues at both transcript and protein levels, and the expression level of MUS81 protein in ovarian cancer cell lines was also higher than that in human normal ovarian surface epithelial cell line (HOSEpiC). We also found that down-regulation of MUS81 expression in ovarian cancer cells inhibited cell proliferation and colony formation ability, and influenced cell cycle progression. Moreover, inhibition of MUS81 expression induced cellular senescence and enhanced the antitumor effect of cisplatin. Down-regulation of MUS81 expression could suppress the growth and development of SOC. These results indicate that MUS81 might play important roles in the progression of SOC and influence the antitumor effect of cisplatin. - Highlights: • MUS81 was overexpression in serous ovarian cancer (SOC). • Meanwhile down-regulation of inhibited cell proliferation and influenced cell cycle progression. • Inhibition of MUS81 induced cell cellular senescence and enhanced the antitumor effect of cisplatin. • Down-regulation of MUS81 expression could suppress the growth and development of SOC.},
doi = {10.1016/J.BBRC.2016.05.152},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 476,
place = {United States},
year = 2016,
month = 8
}
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