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Title: Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling

Abstract

Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor-based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses. -- Highlights: •Minute amount of colonic biopsies by endoscopy is suitable for proteomic analysis. •Centrifugal proteomic reactor can be used for processingmore » tiny clinic biopsy sample. •SOD3 and PRELP are down-regulated in CRC, while NGAL is up-regulated in CRC.« less

Authors:
 [1];  [2];  [1];  [3];  [1]; ;  [4];  [5];  [3];  [1]
  1. Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (China)
  2. Fuyang People’s Hospital (China)
  3. Digestive Endoscopic Center, Shanghai Jiaotong University Affiliated Sixth People’s Hospital (China)
  4. Shanghai Key Laboratory of Diabetes Mellitus, Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital (China)
  5. Department of Biochemistry, Microbiology and Immunology, and Department of Chemistry and Biomolecular Sciences, University of Ottawa (Canada)
Publication Date:
OSTI Identifier:
22598788
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BIOLOGICAL MARKERS; BIOPSY; DIAGNOSIS; MUCOUS MEMBRANES; NEOPLASMS; PROTEINS; SAMPLING; VALIDATION

Citation Formats

Liu, Xing, Xu, Yanli, Meng, Qian, Zheng, Qingqing, Wu, Jianhong, Wang, Chen, Jia, Weiping, Figeys, Daniel, Chang, Ying, E-mail: emulan@163.com, and Zhou, Hu, E-mail: zhouhu@simm.ac.cn. Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.114.
Liu, Xing, Xu, Yanli, Meng, Qian, Zheng, Qingqing, Wu, Jianhong, Wang, Chen, Jia, Weiping, Figeys, Daniel, Chang, Ying, E-mail: emulan@163.com, & Zhou, Hu, E-mail: zhouhu@simm.ac.cn. Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling. United States. doi:10.1016/J.BBRC.2016.05.114.
Liu, Xing, Xu, Yanli, Meng, Qian, Zheng, Qingqing, Wu, Jianhong, Wang, Chen, Jia, Weiping, Figeys, Daniel, Chang, Ying, E-mail: emulan@163.com, and Zhou, Hu, E-mail: zhouhu@simm.ac.cn. 2016. "Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling". United States. doi:10.1016/J.BBRC.2016.05.114.
@article{osti_22598788,
title = {Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling},
author = {Liu, Xing and Xu, Yanli and Meng, Qian and Zheng, Qingqing and Wu, Jianhong and Wang, Chen and Jia, Weiping and Figeys, Daniel and Chang, Ying, E-mail: emulan@163.com and Zhou, Hu, E-mail: zhouhu@simm.ac.cn},
abstractNote = {Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor-based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses. -- Highlights: •Minute amount of colonic biopsies by endoscopy is suitable for proteomic analysis. •Centrifugal proteomic reactor can be used for processing tiny clinic biopsy sample. •SOD3 and PRELP are down-regulated in CRC, while NGAL is up-regulated in CRC.},
doi = {10.1016/J.BBRC.2016.05.114},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 476,
place = {United States},
year = 2016,
month = 8
}