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Title: Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition

Abstract

We have previously reported the co-localization of O-propargyl-puromycin (OP-Puro) with SUMO-2/3 and ubiquitin at promyelocytic leukemia-nuclear bodies (PML-NBs) in the presence of the proteasome inhibitor MG132, implying a role for the ubiquitin family in sequestering OP-puromycylated immature polypeptides to the nucleus during impaired proteasome activity. Here, we found that as expected puromycin induced SUMO-1/2/3 accumulation with ubiquitin at multiple nuclear foci in HeLa cells when co-exposed to MG132. Co-administration of puromycin and MG132 also facilitated redistribution of PML and the SUMO-targeted ubiquitin ligase RNF4 concurrently with SUMO-2/3. As removal of the drugs from the medium led to disappearance of the SUMO-2/3-ubiquitin nuclear foci, our findings indicated that nuclear assembly/disassembly of SUMO-2/3 and ubiquitin was pharmacologically manipulable, supporting our previous observation on OP-Puro, which predicted the ubiquitin family function in sequestrating aberrant proteins to the nucleus. -- Highlights: •Puromycin exhibits the O-propargyl-puromycin effect. •Puromycin induces SUMO redistribution upon proteasome inhibition. •Ubiquitin and RNF4 accumulate at PML-nuclear bodies with SUMO-2/3. •The ubiquitin family may function in nuclear sequestration of immature proteins.

Authors:
 [1];  [1];  [2]
  1. Course for Biological Sciences, Faculty of Science, Kumamoto University, Kumamoto (Japan)
  2. (Japan)
Publication Date:
OSTI Identifier:
22598783
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; HELA CELLS; INHIBITION; LEUKEMIA; LIGASES; POLYPEPTIDES; PUROMYCIN

Citation Formats

Matsumoto, Hotaru, Saitoh, Hisato, E-mail: hisa@kumamoto-u.ac.jp, and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.025.
Matsumoto, Hotaru, Saitoh, Hisato, E-mail: hisa@kumamoto-u.ac.jp, & Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition. United States. doi:10.1016/J.BBRC.2016.05.025.
Matsumoto, Hotaru, Saitoh, Hisato, E-mail: hisa@kumamoto-u.ac.jp, and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Fri . "Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition". United States. doi:10.1016/J.BBRC.2016.05.025.
@article{osti_22598783,
title = {Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition},
author = {Matsumoto, Hotaru and Saitoh, Hisato, E-mail: hisa@kumamoto-u.ac.jp and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto},
abstractNote = {We have previously reported the co-localization of O-propargyl-puromycin (OP-Puro) with SUMO-2/3 and ubiquitin at promyelocytic leukemia-nuclear bodies (PML-NBs) in the presence of the proteasome inhibitor MG132, implying a role for the ubiquitin family in sequestering OP-puromycylated immature polypeptides to the nucleus during impaired proteasome activity. Here, we found that as expected puromycin induced SUMO-1/2/3 accumulation with ubiquitin at multiple nuclear foci in HeLa cells when co-exposed to MG132. Co-administration of puromycin and MG132 also facilitated redistribution of PML and the SUMO-targeted ubiquitin ligase RNF4 concurrently with SUMO-2/3. As removal of the drugs from the medium led to disappearance of the SUMO-2/3-ubiquitin nuclear foci, our findings indicated that nuclear assembly/disassembly of SUMO-2/3 and ubiquitin was pharmacologically manipulable, supporting our previous observation on OP-Puro, which predicted the ubiquitin family function in sequestrating aberrant proteins to the nucleus. -- Highlights: •Puromycin exhibits the O-propargyl-puromycin effect. •Puromycin induces SUMO redistribution upon proteasome inhibition. •Ubiquitin and RNF4 accumulate at PML-nuclear bodies with SUMO-2/3. •The ubiquitin family may function in nuclear sequestration of immature proteins.},
doi = {10.1016/J.BBRC.2016.05.025},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 476,
place = {United States},
year = {Fri Jul 29 00:00:00 EDT 2016},
month = {Fri Jul 29 00:00:00 EDT 2016}
}