skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition

Abstract

We have previously reported the co-localization of O-propargyl-puromycin (OP-Puro) with SUMO-2/3 and ubiquitin at promyelocytic leukemia-nuclear bodies (PML-NBs) in the presence of the proteasome inhibitor MG132, implying a role for the ubiquitin family in sequestering OP-puromycylated immature polypeptides to the nucleus during impaired proteasome activity. Here, we found that as expected puromycin induced SUMO-1/2/3 accumulation with ubiquitin at multiple nuclear foci in HeLa cells when co-exposed to MG132. Co-administration of puromycin and MG132 also facilitated redistribution of PML and the SUMO-targeted ubiquitin ligase RNF4 concurrently with SUMO-2/3. As removal of the drugs from the medium led to disappearance of the SUMO-2/3-ubiquitin nuclear foci, our findings indicated that nuclear assembly/disassembly of SUMO-2/3 and ubiquitin was pharmacologically manipulable, supporting our previous observation on OP-Puro, which predicted the ubiquitin family function in sequestrating aberrant proteins to the nucleus. -- Highlights: •Puromycin exhibits the O-propargyl-puromycin effect. •Puromycin induces SUMO redistribution upon proteasome inhibition. •Ubiquitin and RNF4 accumulate at PML-nuclear bodies with SUMO-2/3. •The ubiquitin family may function in nuclear sequestration of immature proteins.

Authors:
 [1];  [1]
  1. Course for Biological Sciences, Faculty of Science, Kumamoto University, Kumamoto (Japan)
Publication Date:
OSTI Identifier:
22598783
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 476; Journal Issue: 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; HELA CELLS; INHIBITION; LEUKEMIA; LIGASES; POLYPEPTIDES; PUROMYCIN

Citation Formats

Matsumoto, Hotaru, Saitoh, Hisato, and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.025.
Matsumoto, Hotaru, Saitoh, Hisato, & Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition. United States. doi:10.1016/J.BBRC.2016.05.025.
Matsumoto, Hotaru, Saitoh, Hisato, and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto. Fri . "Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition". United States. doi:10.1016/J.BBRC.2016.05.025.
@article{osti_22598783,
title = {Puromycin induces SUMO and ubiquitin redistribution upon proteasome inhibition},
author = {Matsumoto, Hotaru and Saitoh, Hisato and Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto},
abstractNote = {We have previously reported the co-localization of O-propargyl-puromycin (OP-Puro) with SUMO-2/3 and ubiquitin at promyelocytic leukemia-nuclear bodies (PML-NBs) in the presence of the proteasome inhibitor MG132, implying a role for the ubiquitin family in sequestering OP-puromycylated immature polypeptides to the nucleus during impaired proteasome activity. Here, we found that as expected puromycin induced SUMO-1/2/3 accumulation with ubiquitin at multiple nuclear foci in HeLa cells when co-exposed to MG132. Co-administration of puromycin and MG132 also facilitated redistribution of PML and the SUMO-targeted ubiquitin ligase RNF4 concurrently with SUMO-2/3. As removal of the drugs from the medium led to disappearance of the SUMO-2/3-ubiquitin nuclear foci, our findings indicated that nuclear assembly/disassembly of SUMO-2/3 and ubiquitin was pharmacologically manipulable, supporting our previous observation on OP-Puro, which predicted the ubiquitin family function in sequestrating aberrant proteins to the nucleus. -- Highlights: •Puromycin exhibits the O-propargyl-puromycin effect. •Puromycin induces SUMO redistribution upon proteasome inhibition. •Ubiquitin and RNF4 accumulate at PML-nuclear bodies with SUMO-2/3. •The ubiquitin family may function in nuclear sequestration of immature proteins.},
doi = {10.1016/J.BBRC.2016.05.025},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 476,
place = {United States},
year = {2016},
month = {7}
}