skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus

Abstract

We investigated the effect of light spectra on retinal damage and stress in goldfish using green (530 nm) and red (620 nm) light emitting diodes (LEDs) at three intensities each (0.5, 1.0, and 1.5 W/m{sup 2}). We measured the change in the levels of plasma cortisol and H{sub 2}O{sub 2} and expression and levels of caspase-3. The apoptotic response of green and red LED spectra was assessed using the terminal transferase dUTP nick end labeling (TUNEL) assay. Stress indicator (cortisol and H{sub 2}O{sub 2}) and apoptosis-related genes (caspase-3) decreased in green light, but increased in red light with higher light intensities over time. The TUNEL assay revealed that more apoptotic cells were detected in outer nuclear layers after exposure to red LED over time with the increase in light intensity, than the other spectra. These results indicate that green light efficiently reduces retinal damage and stress, whereas red light induces it. Therefore, red light-induced retina damage may induce apoptosis in goldfish retina. -- Highlights: •Green light efficiently reduces retinal damage and stress. •Green spectra reduce caspase production and apoptosis. •Red light-induced retina damage may induce apoptosis in goldfish retina. •The retina of goldfish recognizes green spectra as a stable environment.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22598782
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 476; Journal Issue: 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BIOLOGICAL INDICATORS; BIOLOGICAL STRESS; DAMAGE; GENES; GOLDFISH; HYDROCORTISONE; LABELLING; RETINA; TRANSFERASES

Citation Formats

Song, Jin Ah, Kim, Na Na, Choi, Young Jae, and Choi, Cheol Young, E-mail: choic@kmou.ac.kr. Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.049.
Song, Jin Ah, Kim, Na Na, Choi, Young Jae, & Choi, Cheol Young, E-mail: choic@kmou.ac.kr. Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus. United States. doi:10.1016/J.BBRC.2016.05.049.
Song, Jin Ah, Kim, Na Na, Choi, Young Jae, and Choi, Cheol Young, E-mail: choic@kmou.ac.kr. 2016. "Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus". United States. doi:10.1016/J.BBRC.2016.05.049.
@article{osti_22598782,
title = {Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus},
author = {Song, Jin Ah and Kim, Na Na and Choi, Young Jae and Choi, Cheol Young, E-mail: choic@kmou.ac.kr},
abstractNote = {We investigated the effect of light spectra on retinal damage and stress in goldfish using green (530 nm) and red (620 nm) light emitting diodes (LEDs) at three intensities each (0.5, 1.0, and 1.5 W/m{sup 2}). We measured the change in the levels of plasma cortisol and H{sub 2}O{sub 2} and expression and levels of caspase-3. The apoptotic response of green and red LED spectra was assessed using the terminal transferase dUTP nick end labeling (TUNEL) assay. Stress indicator (cortisol and H{sub 2}O{sub 2}) and apoptosis-related genes (caspase-3) decreased in green light, but increased in red light with higher light intensities over time. The TUNEL assay revealed that more apoptotic cells were detected in outer nuclear layers after exposure to red LED over time with the increase in light intensity, than the other spectra. These results indicate that green light efficiently reduces retinal damage and stress, whereas red light induces it. Therefore, red light-induced retina damage may induce apoptosis in goldfish retina. -- Highlights: •Green light efficiently reduces retinal damage and stress. •Green spectra reduce caspase production and apoptosis. •Red light-induced retina damage may induce apoptosis in goldfish retina. •The retina of goldfish recognizes green spectra as a stable environment.},
doi = {10.1016/J.BBRC.2016.05.049},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 476,
place = {United States},
year = 2016,
month = 7
}