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Title: Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4

Abstract

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and GATA Binding Protein 4 (GATA4) are important for the growth of cardiac fibroblasts (CFs). When deregulated, LOX-1 and GATA4 can cause cardiac remodeling. In the present study, we found novel evidence that GATA4 was required for the LOX-1 regulation of CF proliferation. The inhibition of LOX-1 by RNA interference LOX-1 lentivirus resulted in the loss of PI3K/Akt activation and GATA4 protein expression. The overexpression of LOX-1 by lentivirus rescued CF proliferation, PI3K/Akt activation, and GATA4 protein expression. Moreover, GATA4 overexpression enhanced CF proliferation with LOX-1 inhibition. We also found that the inhibition of PI3K/Akt activation by LY294002, a PI3K inhibitor, reduced cell proliferation and protein level of GATA4. In summary, GATA4 may play an important role in the LOX-1 and PI3K/Akt regulation of CF proliferation. -- Highlights: •GATA4 is regulated by LOX-1 signaling in CFs. •GATA4 is involved in LOX-1 regulating CF proliferation. •GATA4 is regulated by PI3K/Akt signaling in CFs.

Authors:
; ; ; ; ;  [1];  [2];  [1];  [2]
  1. Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou (China)
  2. (China)
Publication Date:
OSTI Identifier:
22598778
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 475; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; FIBROBLASTS; GENE REGULATION; INHIBITION; LECTINS; LIPOPROTEINS; RECEPTORS; RNA

Citation Formats

Liu, Bin, Liu, Ning-Ning, Liu, Wei-Hua, Zhang, Shuang-Wei, Zhang, Jing-Zhi, Li, Ai-Qun, Guangzhou Institute of Cardiovascular Disease, Guangzhou, Liu, Shi-Ming, E-mail: gzliushiming@126.com, and Guangzhou Institute of Cardiovascular Disease, Guangzhou. Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.095.
Liu, Bin, Liu, Ning-Ning, Liu, Wei-Hua, Zhang, Shuang-Wei, Zhang, Jing-Zhi, Li, Ai-Qun, Guangzhou Institute of Cardiovascular Disease, Guangzhou, Liu, Shi-Ming, E-mail: gzliushiming@126.com, & Guangzhou Institute of Cardiovascular Disease, Guangzhou. Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4. United States. doi:10.1016/J.BBRC.2016.05.095.
Liu, Bin, Liu, Ning-Ning, Liu, Wei-Hua, Zhang, Shuang-Wei, Zhang, Jing-Zhi, Li, Ai-Qun, Guangzhou Institute of Cardiovascular Disease, Guangzhou, Liu, Shi-Ming, E-mail: gzliushiming@126.com, and Guangzhou Institute of Cardiovascular Disease, Guangzhou. Fri . "Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4". United States. doi:10.1016/J.BBRC.2016.05.095.
@article{osti_22598778,
title = {Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4},
author = {Liu, Bin and Liu, Ning-Ning and Liu, Wei-Hua and Zhang, Shuang-Wei and Zhang, Jing-Zhi and Li, Ai-Qun and Guangzhou Institute of Cardiovascular Disease, Guangzhou and Liu, Shi-Ming, E-mail: gzliushiming@126.com and Guangzhou Institute of Cardiovascular Disease, Guangzhou},
abstractNote = {Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and GATA Binding Protein 4 (GATA4) are important for the growth of cardiac fibroblasts (CFs). When deregulated, LOX-1 and GATA4 can cause cardiac remodeling. In the present study, we found novel evidence that GATA4 was required for the LOX-1 regulation of CF proliferation. The inhibition of LOX-1 by RNA interference LOX-1 lentivirus resulted in the loss of PI3K/Akt activation and GATA4 protein expression. The overexpression of LOX-1 by lentivirus rescued CF proliferation, PI3K/Akt activation, and GATA4 protein expression. Moreover, GATA4 overexpression enhanced CF proliferation with LOX-1 inhibition. We also found that the inhibition of PI3K/Akt activation by LY294002, a PI3K inhibitor, reduced cell proliferation and protein level of GATA4. In summary, GATA4 may play an important role in the LOX-1 and PI3K/Akt regulation of CF proliferation. -- Highlights: •GATA4 is regulated by LOX-1 signaling in CFs. •GATA4 is involved in LOX-1 regulating CF proliferation. •GATA4 is regulated by PI3K/Akt signaling in CFs.},
doi = {10.1016/J.BBRC.2016.05.095},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 475,
place = {United States},
year = {Fri Jul 08 00:00:00 EDT 2016},
month = {Fri Jul 08 00:00:00 EDT 2016}
}