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Title: ADAM10 is essential for cranial neural crest-derived maxillofacial bone development

Abstract

Growth disorders of the craniofacial bones may lead to craniofacial deformities. The majority of maxillofacial bones are derived from cranial neural crest cells via intramembranous bone formation. Any interruption of the craniofacial skeleton development process might lead to craniofacial malformation. A disintegrin and metalloprotease (ADAM)10 plays an essential role in organ development and tissue integrity in different organs. However, little is known about its function in craniofacial bone formation. Therefore, we investigated the role of ADAM10 in the developing craniofacial skeleton, particularly during typical mandibular bone development. First, we showed that ADAM10 was expressed in a specific area of the craniofacial bone and that the expression pattern dynamically changed during normal mouse craniofacial development. Then, we crossed wnt1-cre transgenic mice with adam10-flox mice to generate ADAM10 conditional knockout mice. The stereomicroscopic, radiographic, and von Kossa staining results showed that conditional knockout of ADAM10 in cranial neural crest cells led to embryonic death, craniofacial dysmorphia and bone defects. Furthermore, we demonstrated that impaired mineralization could be triggered by decreased osteoblast differentiation, increased cell death. Overall, these findings show that ADAM10 plays an essential role in craniofacial bone development. -- Highlights: •We firstly reported that ADAM10 was essentially involved in maxillofacial bonemore » development. •ADAM10 cKO mice present craniofacial dysmorphia and bone defects. •Impaired osteoblast differentiation,proliferation and apoptosis underlie the bone deformity.« less

Authors:
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Publication Date:
OSTI Identifier:
22598777
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 475; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; CELL PROLIFERATION; CONNECTIVE TISSUE CELLS; KNOCK-OUT REACTIONS; MALFORMATIONS; MINERALIZATION; SKELETON; TRANSGENIC MICE

Citation Formats

Tan, Yu, E-mail: tanyu2048@163.com, Fu, Runqing, E-mail: furunqing@sjtu.edu.cn, Liu, Jiaqiang, E-mail: liujqmj@163.com, Wu, Yong, E-mail: wyonger@gmail.com, Wang, Bo, E-mail: wb228@126.com, Jiang, Ning, E-mail: 179639060@qq.com, Nie, Ping, E-mail: nieping1011@sina.com, Cao, Haifeng, E-mail: 0412chf@163.com, Yang, Zhi, E-mail: wcums1981@163.com, and Fang, Bing, E-mail: fangbing@sjtu.edu.cn. ADAM10 is essential for cranial neural crest-derived maxillofacial bone development. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.101.
Tan, Yu, E-mail: tanyu2048@163.com, Fu, Runqing, E-mail: furunqing@sjtu.edu.cn, Liu, Jiaqiang, E-mail: liujqmj@163.com, Wu, Yong, E-mail: wyonger@gmail.com, Wang, Bo, E-mail: wb228@126.com, Jiang, Ning, E-mail: 179639060@qq.com, Nie, Ping, E-mail: nieping1011@sina.com, Cao, Haifeng, E-mail: 0412chf@163.com, Yang, Zhi, E-mail: wcums1981@163.com, & Fang, Bing, E-mail: fangbing@sjtu.edu.cn. ADAM10 is essential for cranial neural crest-derived maxillofacial bone development. United States. doi:10.1016/J.BBRC.2016.05.101.
Tan, Yu, E-mail: tanyu2048@163.com, Fu, Runqing, E-mail: furunqing@sjtu.edu.cn, Liu, Jiaqiang, E-mail: liujqmj@163.com, Wu, Yong, E-mail: wyonger@gmail.com, Wang, Bo, E-mail: wb228@126.com, Jiang, Ning, E-mail: 179639060@qq.com, Nie, Ping, E-mail: nieping1011@sina.com, Cao, Haifeng, E-mail: 0412chf@163.com, Yang, Zhi, E-mail: wcums1981@163.com, and Fang, Bing, E-mail: fangbing@sjtu.edu.cn. Fri . "ADAM10 is essential for cranial neural crest-derived maxillofacial bone development". United States. doi:10.1016/J.BBRC.2016.05.101.
@article{osti_22598777,
title = {ADAM10 is essential for cranial neural crest-derived maxillofacial bone development},
author = {Tan, Yu, E-mail: tanyu2048@163.com and Fu, Runqing, E-mail: furunqing@sjtu.edu.cn and Liu, Jiaqiang, E-mail: liujqmj@163.com and Wu, Yong, E-mail: wyonger@gmail.com and Wang, Bo, E-mail: wb228@126.com and Jiang, Ning, E-mail: 179639060@qq.com and Nie, Ping, E-mail: nieping1011@sina.com and Cao, Haifeng, E-mail: 0412chf@163.com and Yang, Zhi, E-mail: wcums1981@163.com and Fang, Bing, E-mail: fangbing@sjtu.edu.cn},
abstractNote = {Growth disorders of the craniofacial bones may lead to craniofacial deformities. The majority of maxillofacial bones are derived from cranial neural crest cells via intramembranous bone formation. Any interruption of the craniofacial skeleton development process might lead to craniofacial malformation. A disintegrin and metalloprotease (ADAM)10 plays an essential role in organ development and tissue integrity in different organs. However, little is known about its function in craniofacial bone formation. Therefore, we investigated the role of ADAM10 in the developing craniofacial skeleton, particularly during typical mandibular bone development. First, we showed that ADAM10 was expressed in a specific area of the craniofacial bone and that the expression pattern dynamically changed during normal mouse craniofacial development. Then, we crossed wnt1-cre transgenic mice with adam10-flox mice to generate ADAM10 conditional knockout mice. The stereomicroscopic, radiographic, and von Kossa staining results showed that conditional knockout of ADAM10 in cranial neural crest cells led to embryonic death, craniofacial dysmorphia and bone defects. Furthermore, we demonstrated that impaired mineralization could be triggered by decreased osteoblast differentiation, increased cell death. Overall, these findings show that ADAM10 plays an essential role in craniofacial bone development. -- Highlights: •We firstly reported that ADAM10 was essentially involved in maxillofacial bone development. •ADAM10 cKO mice present craniofacial dysmorphia and bone defects. •Impaired osteoblast differentiation,proliferation and apoptosis underlie the bone deformity.},
doi = {10.1016/J.BBRC.2016.05.101},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 475,
place = {United States},
year = {Fri Jul 08 00:00:00 EDT 2016},
month = {Fri Jul 08 00:00:00 EDT 2016}
}