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Title: Ubiquitination of specific mitochondrial matrix proteins

Abstract

Several protein quality control systems in bacteria and/or mitochondrial matrix from lower eukaryotes are absent in higher eukaryotes. These are transfer-messenger RNA (tmRNA), The N-end rule ATP-dependent protease ClpAP, and two more ATP-dependent proteases, HslUV and ClpXP (in yeast). The lost proteases resemble the 26S proteasome and the role of tmRNA and the N-end rule in eukaryotic cytosol is performed by the ubiquitin proteasome system (UPS). Therefore, we hypothesized that the UPS might have substituted these systems – at least partially – in the mitochondrial matrix of higher eukaryotes. Using three independent experimental approaches, we demonstrated the presence of ubiquitinated proteins in the matrix of isolated yeast mitochondria. First, we show that isolated mitochondria contain ubiquitin (Ub) conjugates, which remained intact after trypsin digestion. Second, we demonstrate that the mitochondrial soluble fraction contains Ub-conjugates, several of which were identified by mass spectrometry and are localized to the matrix. Third, using immunoaffinity enrichment by specific antibodies recognizing digested ubiquitinated peptides, we identified a group of Ub-modified matrix proteins. The modification was further substantiated by separation on SDS-PAGE and immunoblots. Last, we attempted to identify the ubiquitin ligase(s) involved, and identified Dma1p as a trypsin-resistant protein in our mitochondrial preparations. Taken together,more » these data suggest a yet undefined role for the UPS in regulation of the mitochondrial matrix proteins. -- Highlights: •Mitochondrial matrix contains ubiquitinated proteins. •Ubiquitination occurs most probably in the matrix. •Dma1p is a ubiquitin ligase present in mitochondrial preparations.« less

Authors:
 [1];  [2];  [1];  [2];  [1];  [1]
  1. The Janet and David Polak Tumor and Vascular Biology Research Center and the Technion Integrated Cancer Center (TICC), The Rappaport Faculty of Medicine and Research Institute, Haifa, 31096 (Israel)
  2. The Smoler Proteomics Center, Faculty of Biology – Technion-Israel Institute of Technology, Haifa, 32000 (Israel)
Publication Date:
OSTI Identifier:
22598769
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 475; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ATP; BACTERIA; CELL PROLIFERATION; DIGESTION; GENE REGULATION; LIGASES; MASS SPECTROSCOPY; MESSENGER-RNA; MITOCHONDRIA; PEPTIDES; QUALITY CONTROL; TRYPSIN; YEASTS

Citation Formats

Lehmann, Gilad, Ziv, Tamar, Braten, Ori, Admon, Arie, Udasin, Ronald G., and Ciechanover, Aaron, E-mail: aaroncie@tx.technion.ac.il. Ubiquitination of specific mitochondrial matrix proteins. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.04.150.
Lehmann, Gilad, Ziv, Tamar, Braten, Ori, Admon, Arie, Udasin, Ronald G., & Ciechanover, Aaron, E-mail: aaroncie@tx.technion.ac.il. Ubiquitination of specific mitochondrial matrix proteins. United States. doi:10.1016/J.BBRC.2016.04.150.
Lehmann, Gilad, Ziv, Tamar, Braten, Ori, Admon, Arie, Udasin, Ronald G., and Ciechanover, Aaron, E-mail: aaroncie@tx.technion.ac.il. Fri . "Ubiquitination of specific mitochondrial matrix proteins". United States. doi:10.1016/J.BBRC.2016.04.150.
@article{osti_22598769,
title = {Ubiquitination of specific mitochondrial matrix proteins},
author = {Lehmann, Gilad and Ziv, Tamar and Braten, Ori and Admon, Arie and Udasin, Ronald G. and Ciechanover, Aaron, E-mail: aaroncie@tx.technion.ac.il},
abstractNote = {Several protein quality control systems in bacteria and/or mitochondrial matrix from lower eukaryotes are absent in higher eukaryotes. These are transfer-messenger RNA (tmRNA), The N-end rule ATP-dependent protease ClpAP, and two more ATP-dependent proteases, HslUV and ClpXP (in yeast). The lost proteases resemble the 26S proteasome and the role of tmRNA and the N-end rule in eukaryotic cytosol is performed by the ubiquitin proteasome system (UPS). Therefore, we hypothesized that the UPS might have substituted these systems – at least partially – in the mitochondrial matrix of higher eukaryotes. Using three independent experimental approaches, we demonstrated the presence of ubiquitinated proteins in the matrix of isolated yeast mitochondria. First, we show that isolated mitochondria contain ubiquitin (Ub) conjugates, which remained intact after trypsin digestion. Second, we demonstrate that the mitochondrial soluble fraction contains Ub-conjugates, several of which were identified by mass spectrometry and are localized to the matrix. Third, using immunoaffinity enrichment by specific antibodies recognizing digested ubiquitinated peptides, we identified a group of Ub-modified matrix proteins. The modification was further substantiated by separation on SDS-PAGE and immunoblots. Last, we attempted to identify the ubiquitin ligase(s) involved, and identified Dma1p as a trypsin-resistant protein in our mitochondrial preparations. Taken together, these data suggest a yet undefined role for the UPS in regulation of the mitochondrial matrix proteins. -- Highlights: •Mitochondrial matrix contains ubiquitinated proteins. •Ubiquitination occurs most probably in the matrix. •Dma1p is a ubiquitin ligase present in mitochondrial preparations.},
doi = {10.1016/J.BBRC.2016.04.150},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 475,
place = {United States},
year = {Fri Jun 17 00:00:00 EDT 2016},
month = {Fri Jun 17 00:00:00 EDT 2016}
}