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Title: Suppressive effects of lysozyme on polyphosphate-mediated vascular inflammatory responses

Abstract

Lysozyme, found in relatively high concentration in blood, saliva, tears, and milk, protects us from the ever-present danger of bacterial infection. Previous studies have reported proinflammatory responses of endothelial cells to the release of polyphosphate(PolyP). In this study, we examined the anti-inflammatory responses and mechanisms of lysozyme and its effects on PolyP-induced septic activities in human umbilical vein endothelial cells (HUVECs) and mice. The survival rates, septic biomarker levels, behavior of human neutrophils, and vascular permeability were determined in PolyP-activated HUVECs and mice. Lysozyme suppressed the PolyP-mediated vascular barrier permeability, upregulation of inflammatory biomarkers, adhesion/migration of leukocytes, and activation and/or production of nuclear factor-κB, tumor necrosis factor-α, and interleukin-6. Furthermore, lysozyme demonstrated protective effects on PolyP-mediated lethal death and the levels of the related septic biomarkers. Therefore, these results indicated the therapeutic potential of lysozyme on various systemic inflammatory diseases, such as sepsis or septic shock. -- Highlights: •PolyP is shown to be an important mediator of vascular inflammation. •Lysozyme inhibited PolyP-mediated hyperpermeability. •Lysozyme inhibited PolyP-mediated septic response. •Lysozyme reduced PolyP-induced septic mortality.

Authors:
 [1];  [2];  [1];  [1]
  1. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, Kyungpook National University, Daegu 41566 (Korea, Republic of)
  2. Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22598767
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 474; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; CONCENTRATION RATIO; INFLAMMATION; LYMPHOKINES; LYSOZYME; MICE; MILK; MORTALITY; NECROSIS; NEOPLASMS; NEUTROPHILS; PERMEABILITY; SALIVA; VEINS

Citation Formats

Chung, Jiwoo, Ku, Sae-Kwang, Lee, Suyeon, and Bae, Jong-Sup, E-mail: baejs@knu.ac.kr. Suppressive effects of lysozyme on polyphosphate-mediated vascular inflammatory responses. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.05.016.
Chung, Jiwoo, Ku, Sae-Kwang, Lee, Suyeon, & Bae, Jong-Sup, E-mail: baejs@knu.ac.kr. Suppressive effects of lysozyme on polyphosphate-mediated vascular inflammatory responses. United States. doi:10.1016/J.BBRC.2016.05.016.
Chung, Jiwoo, Ku, Sae-Kwang, Lee, Suyeon, and Bae, Jong-Sup, E-mail: baejs@knu.ac.kr. Fri . "Suppressive effects of lysozyme on polyphosphate-mediated vascular inflammatory responses". United States. doi:10.1016/J.BBRC.2016.05.016.
@article{osti_22598767,
title = {Suppressive effects of lysozyme on polyphosphate-mediated vascular inflammatory responses},
author = {Chung, Jiwoo and Ku, Sae-Kwang and Lee, Suyeon and Bae, Jong-Sup, E-mail: baejs@knu.ac.kr},
abstractNote = {Lysozyme, found in relatively high concentration in blood, saliva, tears, and milk, protects us from the ever-present danger of bacterial infection. Previous studies have reported proinflammatory responses of endothelial cells to the release of polyphosphate(PolyP). In this study, we examined the anti-inflammatory responses and mechanisms of lysozyme and its effects on PolyP-induced septic activities in human umbilical vein endothelial cells (HUVECs) and mice. The survival rates, septic biomarker levels, behavior of human neutrophils, and vascular permeability were determined in PolyP-activated HUVECs and mice. Lysozyme suppressed the PolyP-mediated vascular barrier permeability, upregulation of inflammatory biomarkers, adhesion/migration of leukocytes, and activation and/or production of nuclear factor-κB, tumor necrosis factor-α, and interleukin-6. Furthermore, lysozyme demonstrated protective effects on PolyP-mediated lethal death and the levels of the related septic biomarkers. Therefore, these results indicated the therapeutic potential of lysozyme on various systemic inflammatory diseases, such as sepsis or septic shock. -- Highlights: •PolyP is shown to be an important mediator of vascular inflammation. •Lysozyme inhibited PolyP-mediated hyperpermeability. •Lysozyme inhibited PolyP-mediated septic response. •Lysozyme reduced PolyP-induced septic mortality.},
doi = {10.1016/J.BBRC.2016.05.016},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 474,
place = {United States},
year = {Fri Jun 10 00:00:00 EDT 2016},
month = {Fri Jun 10 00:00:00 EDT 2016}
}