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Title: ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus

Abstract

Starch is the main storage compound in underground organs like corms. ADP-glucose pyrophosphorylase (AGPase) plays a key role in regulating starch biosynthesis in storage organs and is likely one of the most important determinant of sink strength. Here, we identify an AGPase gene (GhAGPS1) from gladiolus. The highest transcriptional levels of GhAGPS1 were observed in cormels and corms. Transformation of GhAGPS1 into Arabidopsis rescued the phenotype of aps1 mutant. Silencing GhAGPS1 in gladiolus corms by virus-induced gene silencing (VIGS) decreased the transcriptional levels of two genes and starch content. Transmission electron microscopy analyses of leaf and corm sections confirmed that starch biosynthesis was inhibited. Corm weight and cormel number reduced significantly in the silenced plants. Taken together, these results indicate that inhibiting the expression of AGPase gene could impair starch synthesis, which results in the lowered corm quality and cormel yield in gladiolus. -- Highlights: •Cormel quantity was reduced significantly in silenced Gladiolus plants. •Corm quality was declined significantly in silenced Gladiolus plants. •Starch synthesis was inhibited in silenced Gladiolus plants.

Authors:
 [1];  [1];  [1];  [2]; ; ; ; ; ; ;  [1];  [1]
  1. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Yuan Mingyuan Western Road 2#, Beijing 100193 (China)
  2. (China)
Publication Date:
OSTI Identifier:
22598737
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 474; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADP; ARABIDOPSIS; BIOSYNTHESIS; GENES; GLUCOSE; MUTANTS; ORGANS; PHENOTYPE; STARCH; TRANSFORMATIONS; TRANSMISSION ELECTRON MICROSCOPY; VIRUSES

Citation Formats

Seng, Shanshan, E-mail: seshsh108@126.com, Wu, Jian, Sui, Juanjuan, College of Biology, Fuyang Normal College, Qinghe Western Road 100#, Fuyang 236037, Anhui, Wu, Chenyu, Zhong, Xionghui, Liu, Chen, Liu, Chao, Gong, Benhe, Zhang, Fengqin, He, Junna, and Yi, Mingfang, E-mail: ymfang@cau.edu.cn. ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.04.103.
Seng, Shanshan, E-mail: seshsh108@126.com, Wu, Jian, Sui, Juanjuan, College of Biology, Fuyang Normal College, Qinghe Western Road 100#, Fuyang 236037, Anhui, Wu, Chenyu, Zhong, Xionghui, Liu, Chen, Liu, Chao, Gong, Benhe, Zhang, Fengqin, He, Junna, & Yi, Mingfang, E-mail: ymfang@cau.edu.cn. ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus. United States. doi:10.1016/J.BBRC.2016.04.103.
Seng, Shanshan, E-mail: seshsh108@126.com, Wu, Jian, Sui, Juanjuan, College of Biology, Fuyang Normal College, Qinghe Western Road 100#, Fuyang 236037, Anhui, Wu, Chenyu, Zhong, Xionghui, Liu, Chen, Liu, Chao, Gong, Benhe, Zhang, Fengqin, He, Junna, and Yi, Mingfang, E-mail: ymfang@cau.edu.cn. Fri . "ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus". United States. doi:10.1016/J.BBRC.2016.04.103.
@article{osti_22598737,
title = {ADP-glucose pyrophosphorylase gene plays a key role in the quality of corm and yield of cormels in gladiolus},
author = {Seng, Shanshan, E-mail: seshsh108@126.com and Wu, Jian and Sui, Juanjuan and College of Biology, Fuyang Normal College, Qinghe Western Road 100#, Fuyang 236037, Anhui and Wu, Chenyu and Zhong, Xionghui and Liu, Chen and Liu, Chao and Gong, Benhe and Zhang, Fengqin and He, Junna and Yi, Mingfang, E-mail: ymfang@cau.edu.cn},
abstractNote = {Starch is the main storage compound in underground organs like corms. ADP-glucose pyrophosphorylase (AGPase) plays a key role in regulating starch biosynthesis in storage organs and is likely one of the most important determinant of sink strength. Here, we identify an AGPase gene (GhAGPS1) from gladiolus. The highest transcriptional levels of GhAGPS1 were observed in cormels and corms. Transformation of GhAGPS1 into Arabidopsis rescued the phenotype of aps1 mutant. Silencing GhAGPS1 in gladiolus corms by virus-induced gene silencing (VIGS) decreased the transcriptional levels of two genes and starch content. Transmission electron microscopy analyses of leaf and corm sections confirmed that starch biosynthesis was inhibited. Corm weight and cormel number reduced significantly in the silenced plants. Taken together, these results indicate that inhibiting the expression of AGPase gene could impair starch synthesis, which results in the lowered corm quality and cormel yield in gladiolus. -- Highlights: •Cormel quantity was reduced significantly in silenced Gladiolus plants. •Corm quality was declined significantly in silenced Gladiolus plants. •Starch synthesis was inhibited in silenced Gladiolus plants.},
doi = {10.1016/J.BBRC.2016.04.103},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 474,
place = {United States},
year = {2016},
month = {5}
}