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Title: A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters

Abstract

Scanning speed and coupling efficiency of excitation light to optic fibres are two major technical challenges that limit the potential of fluorescence excitation-emission matrix (EEM) spectrometer for on-line applications and in vivo studies. In this paper, a novel EEM system, utilizing a supercontinuum white light source and acousto-optic tunable filters (AOTFs), was introduced and evaluated. The supercontinuum white light, generated by pumping a nonlinear photonic crystal fiber with an 800 nm femtosecond laser, was efficiently coupled into a bifurcated optic fiber bundle. High speed EEM spectral scanning was achieved using AOTFs both for selecting excitation wavelength and scanning emission spectra. Using calibration lamps (neon and mercury argon), wavelength deviations were determined to vary from 0.18 nm to −0.70 nm within the spectral range of 500–850 nm. Spectral bandwidth for filtered excitation light broadened by twofold compared to that measured with monochromatic light between 650 nm and 750 nm. The EEM spectra for methanol solutions of laser dyes were successfully acquired with this rapid fluorometer using an integration time of 5 s.

Authors:
 [1];  [2];  [2]; ; ; ;  [1];  [2]
  1. Imaging Unit, Integrative Oncology Department, BC Cancer Agency Research Center, 675 West 10th Avenue, Vancouver, British Columbia V5Z 1L3 (Canada)
  2. (Canada)
Publication Date:
OSTI Identifier:
22597908
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 87; Journal Issue: 6; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ARGON; COMPARATIVE EVALUATIONS; EMISSION SPECTRA; EXCITATION; FIBERS; FILTERS; FLUORESCENCE; FLUORIMETERS; IN VIVO; LASERS; LIGHT BULBS; LIGHT SOURCES; MERCURY; METHANOL; MONOCHROMATIC RADIATION; NEON; NONLINEAR PROBLEMS; SPECTROMETERS; VELOCITY; WAVELENGTHS

Citation Formats

Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters. United States: N. p., 2016. Web. doi:10.1063/1.4954502.
Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, & Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters. United States. doi:10.1063/1.4954502.
Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. Wed . "A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters". United States. doi:10.1063/1.4954502.
@article{osti_22597908,
title = {A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters},
author = {Wang, Wenbo and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8 and Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4 and Wu, Zhenguo and Zhao, Jianhua and Lui, Harvey and Zeng, Haishan, E-mail: hzeng@bccrc.ca and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8},
abstractNote = {Scanning speed and coupling efficiency of excitation light to optic fibres are two major technical challenges that limit the potential of fluorescence excitation-emission matrix (EEM) spectrometer for on-line applications and in vivo studies. In this paper, a novel EEM system, utilizing a supercontinuum white light source and acousto-optic tunable filters (AOTFs), was introduced and evaluated. The supercontinuum white light, generated by pumping a nonlinear photonic crystal fiber with an 800 nm femtosecond laser, was efficiently coupled into a bifurcated optic fiber bundle. High speed EEM spectral scanning was achieved using AOTFs both for selecting excitation wavelength and scanning emission spectra. Using calibration lamps (neon and mercury argon), wavelength deviations were determined to vary from 0.18 nm to −0.70 nm within the spectral range of 500–850 nm. Spectral bandwidth for filtered excitation light broadened by twofold compared to that measured with monochromatic light between 650 nm and 750 nm. The EEM spectra for methanol solutions of laser dyes were successfully acquired with this rapid fluorometer using an integration time of 5 s.},
doi = {10.1063/1.4954502},
journal = {Review of Scientific Instruments},
number = 6,
volume = 87,
place = {United States},
year = {Wed Jun 15 00:00:00 EDT 2016},
month = {Wed Jun 15 00:00:00 EDT 2016}
}