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Title: A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters

Abstract

Scanning speed and coupling efficiency of excitation light to optic fibres are two major technical challenges that limit the potential of fluorescence excitation-emission matrix (EEM) spectrometer for on-line applications and in vivo studies. In this paper, a novel EEM system, utilizing a supercontinuum white light source and acousto-optic tunable filters (AOTFs), was introduced and evaluated. The supercontinuum white light, generated by pumping a nonlinear photonic crystal fiber with an 800 nm femtosecond laser, was efficiently coupled into a bifurcated optic fiber bundle. High speed EEM spectral scanning was achieved using AOTFs both for selecting excitation wavelength and scanning emission spectra. Using calibration lamps (neon and mercury argon), wavelength deviations were determined to vary from 0.18 nm to −0.70 nm within the spectral range of 500–850 nm. Spectral bandwidth for filtered excitation light broadened by twofold compared to that measured with monochromatic light between 650 nm and 750 nm. The EEM spectra for methanol solutions of laser dyes were successfully acquired with this rapid fluorometer using an integration time of 5 s.

Authors:
 [1];  [2];  [2]; ; ; ;  [1];  [2]
  1. Imaging Unit, Integrative Oncology Department, BC Cancer Agency Research Center, 675 West 10th Avenue, Vancouver, British Columbia V5Z 1L3 (Canada)
  2. (Canada)
Publication Date:
OSTI Identifier:
22597908
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 87; Journal Issue: 6; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ARGON; COMPARATIVE EVALUATIONS; EMISSION SPECTRA; EXCITATION; FIBERS; FILTERS; FLUORESCENCE; FLUORIMETERS; IN VIVO; LASERS; LIGHT BULBS; LIGHT SOURCES; MERCURY; METHANOL; MONOCHROMATIC RADIATION; NEON; NONLINEAR PROBLEMS; SPECTROMETERS; VELOCITY; WAVELENGTHS

Citation Formats

Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters. United States: N. p., 2016. Web. doi:10.1063/1.4954502.
Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, & Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters. United States. doi:10.1063/1.4954502.
Wang, Wenbo, Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8, Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Wu, Zhenguo, Zhao, Jianhua, Lui, Harvey, Zeng, Haishan, E-mail: hzeng@bccrc.ca, and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8. 2016. "A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters". United States. doi:10.1063/1.4954502.
@article{osti_22597908,
title = {A rapid excitation-emission matrix fluorometer utilizing supercontinuum white light and acousto-optic tunable filters},
author = {Wang, Wenbo and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8 and Department of Biomedical Engineering, University of British Columbia, KAIS 5500, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4 and Wu, Zhenguo and Zhao, Jianhua and Lui, Harvey and Zeng, Haishan, E-mail: hzeng@bccrc.ca and Department of Dermatology and Skin Science, University of British Columbia, 835 West 10th Avenue, Vancouver, British Columbia V5Z 4E8},
abstractNote = {Scanning speed and coupling efficiency of excitation light to optic fibres are two major technical challenges that limit the potential of fluorescence excitation-emission matrix (EEM) spectrometer for on-line applications and in vivo studies. In this paper, a novel EEM system, utilizing a supercontinuum white light source and acousto-optic tunable filters (AOTFs), was introduced and evaluated. The supercontinuum white light, generated by pumping a nonlinear photonic crystal fiber with an 800 nm femtosecond laser, was efficiently coupled into a bifurcated optic fiber bundle. High speed EEM spectral scanning was achieved using AOTFs both for selecting excitation wavelength and scanning emission spectra. Using calibration lamps (neon and mercury argon), wavelength deviations were determined to vary from 0.18 nm to −0.70 nm within the spectral range of 500–850 nm. Spectral bandwidth for filtered excitation light broadened by twofold compared to that measured with monochromatic light between 650 nm and 750 nm. The EEM spectra for methanol solutions of laser dyes were successfully acquired with this rapid fluorometer using an integration time of 5 s.},
doi = {10.1063/1.4954502},
journal = {Review of Scientific Instruments},
number = 6,
volume = 87,
place = {United States},
year = 2016,
month = 6
}
  • Optical multipass schemes of the interaction of light and sound, which are promising for filtration of optical beams based on tunable acousto-optic filters, are studied. The features of operation of acousto-optic filters in the rejection and transmission regimes are considered. It is proved theoretically and confirmed experimentally that the use of multiple interaction improves the spectral and energy parameters of acousto-optic devices. The collinear and transverse geometry of acousto-optic interaction in cells based on a paratellurite crystal is studied in the double-pass, three-pass, and multipass diffraction regimes. (control of laser radiation parameters)
  • A new luminescence spectrometer based on quartz-collinear acousto-optic tunable filters (AOTFs) and capable of synchronous scanning is described. An acousto-optic tunable filter is an electronically tunable optical bandpass filter. Unlike a tunable grating monochromator, an AOTF has no moving mechanical parts, and an AOTF can be tuned to any wavelength within its operating range in microseconds. These characteristics, combined with the small size of these devices, make AOTFs an important new alternative to conventional monochromators, especially for portable instrumentation. The relevant performance of the AOTFs (efficiency, bandwidth, rejection, etc.) is compared with that of typical small-grating monochromator. {copyright} {ital 1995more » Society for Applied Spectroscopy.}« less
  • Quasi-collinear acousto-optic interaction is studied in acoustically and optically anisotropic paratellurite crystals. The possible applications of this interaction in acousto-optic tunable filters with a high spectral resolution are discussed. Different modifications of devices are compared and variants of devices intended for processing light beams and selection of light signals in fibreoptic communication systems with wavelength division multiplexing (WDM) at {lambda} {approx_equal} 1550 nm are considered. (light modulation)
  • A compact, solid-state, high-resolution spectrometer consisting of an acousto-optic tunable filter (AOTF) and a fiber-optic Fabry{endash}Perot (FFP) interferometer has been developed. The system has been designed for high-resolution inductively coupled plasma atomic emission spectroscopy (ICP-AES) applications. A description of the AOTF-FFP and its performance is presented. The resolution of the AOTF-FFP was determined by measuring the physical widths of ICP emission lines using a 1.5-m-focal-length grating spectrometer and deconvoluting the physical line shapes from the acquired AOTF-FFP spectra. Over the optimum range of the FFP mirror coatings, the resolution is sufficient for the determination of isotopic and hyperfine emission featuresmore » in ICP-AES experiments, and approaches that of the 1.5-m spectrometer. The application of the AOTF-FFP to the determination of uranium isotopes (U-235 and U-238) introduced into the ICP is presented. {copyright} {ital 1996 Society for Applied Spectroscopy.}« less
  • This paper describes a new fiber-optic multisensor based on an acousto-optic tunable filter (AOTF) and capable of remote sensing using a multioptical fiber array (MOFA). A two-dimensional charge-coupled device (CCD) was used as a detector, and the AOTF was used as a wavelength selector. Unlike a tunable grating or prism-based monochromator, an AOTF has no moving parts, and an AOTF can be rapidly tuned to any wavelength in its operating range within microseconds. The large aperture of the AOTF allows the optical signal from over 100 fiber-optic sensors to be measured simultaneously. These characteristics, combined with their small size, makemore » AOTFs an important new alternative to conventional monochromators, especially for spectral multisensing and imaging. A prototype fiber-optic multisensor system has been developed, and its feasibility for simultaneous detection of molecular luminescence signal via fiber-optic probes is demonstrated. {copyright} {ital 1996} {ital Society for Applied Spectroscopy}« less