Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes

Xie, Xin; Dai, Hui; Zhuang, Binyu; Chai, Li; Xie, Yanguang; Li, Yuzhen

doi:10.1016/J.BBRC.2016.01.047

Title: Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes

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Abstract

The effects and the underlying mechanisms of hydrogen sulfide (H{sub 2}S) on keratinocyte proliferation and differentiation are still less known. In the current study, we investigated the effects and the underlying mechanisms of exogenous H{sub 2}S on keratinocyte proliferation and differentiation. Human keratinocytes (HaCaT cells) were treated with various concentrations (0.05, 0.25, 0.5 and 1 mM) of sodium hydrosulfide (NaHS, a donor of H{sub 2}S) for 24 h. A CCK-8 assay was used to assess cell viability. Western blot analysis was performed to determine the expression levels of proteins associated with differentiation and autophagy. Transmission electron microscopy was performed to observe autophagic vacuoles, and flow cytometry was applied to evaluate apoptosis. NaHS promoted the viability, induced the differentiation, and enhanced autophagic activity in a dose-dependent manner in HaCaT cells but had no effect on cell apoptosis. Blockage of autophagy by ATG5 siRNA inhibited NaHS-induced cell proliferation and differentiation. The current study demonstrated that autophagy in response to exogenous H{sub 2}S treatment promoted keratinocyte proliferation and differentiation. Our results provide additional insights into the potential role of autophagy in keratinocyte proliferation and differentiation. - Highlights: • Exogenous H{sub 2}S promotes keratinocyte proliferation and differentiation. • The effects of H{sub 2}S on proliferation andmore »« less

Authors:

Xie, Xin ^[1]; Dai, Hui ^[2]; Zhuang, Binyu ^[1]; Chai, Li; Xie, Yanguang ^[3]; Li, Yuzhen ^[1]

Department of Dermatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang Province (China)
Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang Province (China)
Institute of Dermatology of Heilongjiang Province, Harbin, 150001, Heilongjiang Province (China)

Publication Date:: Fri Apr 08 00:00:00 EDT 2016

OSTI Identifier:: 22596318

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 472; Journal Issue: 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; AMP; APOPTOSIS; CELL PROLIFERATION; CONCENTRATION RATIO; HYDROGEN SULFIDES; KERATIN; RNA; SODIUM HYDRIDES; TRANSMISSION ELECTRON MICROSCOPY

Citation Formats


                    Xie, Xin, Dai, Hui, Zhuang, Binyu, Chai, Li, Xie, Yanguang, and Li, Yuzhen. Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes.  United States: N. p., 2016. 
        Web.  doi:10.1016/J.BBRC.2016.01.047.

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                    Xie, Xin, Dai, Hui, Zhuang, Binyu, Chai, Li, Xie, Yanguang, & Li, Yuzhen. Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes.  United States.  https://doi.org/10.1016/J.BBRC.2016.01.047

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                    Xie, Xin, Dai, Hui, Zhuang, Binyu, Chai, Li, Xie, Yanguang, and Li, Yuzhen. 2016.  
        "Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes".  United States.  https://doi.org/10.1016/J.BBRC.2016.01.047.

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@article{osti_22596318,

  title        = {Exogenous hydrogen sulfide promotes cell proliferation and differentiation by modulating autophagy in human keratinocytes},

  author       = {Xie, Xin and Dai, Hui and Zhuang, Binyu and Chai, Li and Xie, Yanguang and Li, Yuzhen},

  abstractNote = {The effects and the underlying mechanisms of hydrogen sulfide (H{sub 2}S) on keratinocyte proliferation and differentiation are still less known. In the current study, we investigated the effects and the underlying mechanisms of exogenous H{sub 2}S on keratinocyte proliferation and differentiation. Human keratinocytes (HaCaT cells) were treated with various concentrations (0.05, 0.25, 0.5 and 1 mM) of sodium hydrosulfide (NaHS, a donor of H{sub 2}S) for 24 h. A CCK-8 assay was used to assess cell viability. Western blot analysis was performed to determine the expression levels of proteins associated with differentiation and autophagy. Transmission electron microscopy was performed to observe autophagic vacuoles, and flow cytometry was applied to evaluate apoptosis. NaHS promoted the viability, induced the differentiation, and enhanced autophagic activity in a dose-dependent manner in HaCaT cells but had no effect on cell apoptosis. Blockage of autophagy by ATG5 siRNA inhibited NaHS-induced cell proliferation and differentiation. The current study demonstrated that autophagy in response to exogenous H{sub 2}S treatment promoted keratinocyte proliferation and differentiation. Our results provide additional insights into the potential role of autophagy in keratinocyte proliferation and differentiation. - Highlights: • Exogenous H{sub 2}S promotes keratinocyte proliferation and differentiation. • The effects of H{sub 2}S on proliferation and differentiation is modulated by autophagy. • Exogenous H{sub 2}S has no effect on keratinocyte apoptosis.},

  doi          = {10.1016/J.BBRC.2016.01.047},

  url          = {https://www.osti.gov/biblio/22596318},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 3,

  volume       = 472,

  place        = {United States},

  year         = {Fri Apr 08 00:00:00 EDT 2016},

  month        = {Fri Apr 08 00:00:00 EDT 2016}

}

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