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Title: Gamma-glutamylcyclotransferase promotes the growth of human glioma cells by activating Notch-Akt signaling

Abstract

Glioma as an aggressive type tumor is rapidly growing and has become one of the leading cause of cancer-related death worldwide. γ-Glutamylcyclotransferase (GGCT) has been shown as a diagnostic marker in various cancers. To reveal whether there is a correlation between GGCT and human glioma, GGCT expression in human glioma tissues and cell lines was first determined. We found that GGCT expression was up-regulated in human glioma tissues and cell lines. Further, we demonstrate that GGCT knockdown inhibits glioma cell T98G and U251 proliferation and colony formation, whereas GGCT overexpression leads to oppose effects. GGCT overexpression promotes the expression of Notch receptors and activates Akt signaling in glioma cells, and Notch-Akt signaling is activated in glioma tissues with high expression of GGCT. Finally, we show that inhibition of Notch-Akt signaling with Notch inhibitor MK-0752 blocks the effects of GGCT on glioma proliferation and colony formation. In conclusion, GGCT plays a critical role in glioma cell proliferation and may be a potential cancer therapeutic target. - Highlights: • GGCT expression is up-regulated in human glioma tissues and cell lines. • GGCT promotes glioma cell growth and colony formation. • GGCT promotes the activation of Notch-Akt signaling in glioma cells and tissues.more » • Notch inhibition blocks the role of GGCT in human glioma cells.« less

Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
22596300
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 471; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; COLONY FORMATION; CORRELATIONS; DEATH; GLIOMAS; INHIBITION; RECEPTORS

Citation Formats

Shen, Shang-Hang, Yu, Ning, Liu, Xi-Yao, Tan, Guo-Wei, and Wang, Zhan-Xiang, E-mail: md_wzx7189@163.com. Gamma-glutamylcyclotransferase promotes the growth of human glioma cells by activating Notch-Akt signaling. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.01.165.
Shen, Shang-Hang, Yu, Ning, Liu, Xi-Yao, Tan, Guo-Wei, & Wang, Zhan-Xiang, E-mail: md_wzx7189@163.com. Gamma-glutamylcyclotransferase promotes the growth of human glioma cells by activating Notch-Akt signaling. United States. doi:10.1016/J.BBRC.2016.01.165.
Shen, Shang-Hang, Yu, Ning, Liu, Xi-Yao, Tan, Guo-Wei, and Wang, Zhan-Xiang, E-mail: md_wzx7189@163.com. Fri . "Gamma-glutamylcyclotransferase promotes the growth of human glioma cells by activating Notch-Akt signaling". United States. doi:10.1016/J.BBRC.2016.01.165.
@article{osti_22596300,
title = {Gamma-glutamylcyclotransferase promotes the growth of human glioma cells by activating Notch-Akt signaling},
author = {Shen, Shang-Hang and Yu, Ning and Liu, Xi-Yao and Tan, Guo-Wei and Wang, Zhan-Xiang, E-mail: md_wzx7189@163.com},
abstractNote = {Glioma as an aggressive type tumor is rapidly growing and has become one of the leading cause of cancer-related death worldwide. γ-Glutamylcyclotransferase (GGCT) has been shown as a diagnostic marker in various cancers. To reveal whether there is a correlation between GGCT and human glioma, GGCT expression in human glioma tissues and cell lines was first determined. We found that GGCT expression was up-regulated in human glioma tissues and cell lines. Further, we demonstrate that GGCT knockdown inhibits glioma cell T98G and U251 proliferation and colony formation, whereas GGCT overexpression leads to oppose effects. GGCT overexpression promotes the expression of Notch receptors and activates Akt signaling in glioma cells, and Notch-Akt signaling is activated in glioma tissues with high expression of GGCT. Finally, we show that inhibition of Notch-Akt signaling with Notch inhibitor MK-0752 blocks the effects of GGCT on glioma proliferation and colony formation. In conclusion, GGCT plays a critical role in glioma cell proliferation and may be a potential cancer therapeutic target. - Highlights: • GGCT expression is up-regulated in human glioma tissues and cell lines. • GGCT promotes glioma cell growth and colony formation. • GGCT promotes the activation of Notch-Akt signaling in glioma cells and tissues. • Notch inhibition blocks the role of GGCT in human glioma cells.},
doi = {10.1016/J.BBRC.2016.01.165},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 471,
place = {United States},
year = {Fri Mar 18 00:00:00 EDT 2016},
month = {Fri Mar 18 00:00:00 EDT 2016}
}