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Title: Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens

Abstract

Desiccation tolerance is an ancestral feature of land plants and is still retained in non-vascular plants such as bryophytes and some vascular plants. However, except for seeds and spores, this trait is absent in vegetative tissues of vascular plants. Although many studies have focused on understanding the molecular basis underlying desiccation tolerance using transcriptome and proteome approaches, the critical molecular differences between desiccation tolerant plants and non-desiccation plants are still not clear. The moss Physcomitrella patens cannot survive rapid desiccation under laboratory conditions, but if cells of the protonemata are treated by the phytohormone abscisic acid (ABA) prior to desiccation, it can survive 24 h exposure to desiccation and regrow after rehydration. The desiccation tolerance induced by ABA (AiDT) is specific to this hormone, but also depends on a plant transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3). Here we report the comparative proteomic analysis of AiDT between wild type and ABI3 deleted mutant (Δabi3) of P. patens using iTRAQ (Isobaric Tags for Relative and Absolute Quantification). From a total of 1980 unique proteins that we identified, only 16 proteins are significantly altered in Δabi3 compared to wild type after desiccation following ABA treatment. Among this group, three of the four proteins that weremore » severely affected in Δabi3 tissue were Arabidopsis orthologous genes, which were expressed in maturing seeds under the regulation of ABI3. These included a Group 1 late embryogenesis abundant (LEA) protein, a short-chain dehydrogenase, and a desiccation-related protein. Our results suggest that at least three of these proteins expressed in desiccation tolerant cells of both Arabidopsis and the moss are very likely to play important roles in acquisition of desiccation tolerance in land plants. Furthermore, our results suggest that the regulatory machinery of ABA- and ABI3-mediated gene expression for desiccation tolerance might have evolved in ancestral land plants before the separation of bryophytes and vascular plants. - Highlights: • Large-scale proteomics highlighted proteins related to plant desiccation tolerance. • The proteins were regulated by both the phytohormone ABA and ABI3. • The proteins accumulated in desiccation tolerant cells of both Arabidopsis and moss. • Evolutionary origin of regulatory machinery for desiccation tolerance is proposed.« less

Authors:
 [1];  [2];  [2];  [1];  [1];  [1];  [1]
  1. Department of BioScience, Tokyo University of Agriculture 1-1-1 Sakuragaoka, Setagayaku, Tokyo, 156-8502 (Japan)
  2. Laboratory of Immune Signal, National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8 Saito-Asagi, Ibaraki, Osaka, 567-0085 (Japan)
Publication Date:
OSTI Identifier:
22596299
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 471; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSCISIC ACID; ANIMAL TISSUES; ARABIDOPSIS; CELL PROLIFERATION; GENE REGULATION; HORMONES; MOSSES; MUTANTS; SPORES; TOLERANCE; TRANSCRIPTION; TRANSCRIPTION FACTORS

Citation Formats

Yotsui, Izumi, Serada, Satoshi, Naka, Tetsuji, Saruhashi, Masashi, Taji, Teruaki, Hayashi, Takahisa, Quatrano, Ralph S., E-mail: rsq@wustl.edu, and Sakata, Yoichi. Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.02.024.
Yotsui, Izumi, Serada, Satoshi, Naka, Tetsuji, Saruhashi, Masashi, Taji, Teruaki, Hayashi, Takahisa, Quatrano, Ralph S., E-mail: rsq@wustl.edu, & Sakata, Yoichi. Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens. United States. doi:10.1016/J.BBRC.2016.02.024.
Yotsui, Izumi, Serada, Satoshi, Naka, Tetsuji, Saruhashi, Masashi, Taji, Teruaki, Hayashi, Takahisa, Quatrano, Ralph S., E-mail: rsq@wustl.edu, and Sakata, Yoichi. Fri . "Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens". United States. doi:10.1016/J.BBRC.2016.02.024.
@article{osti_22596299,
title = {Large-scale proteome analysis of abscisic acid and ABSCISIC ACID INSENSITIVE3-dependent proteins related to desiccation tolerance in Physcomitrella patens},
author = {Yotsui, Izumi and Serada, Satoshi and Naka, Tetsuji and Saruhashi, Masashi and Taji, Teruaki and Hayashi, Takahisa and Quatrano, Ralph S., E-mail: rsq@wustl.edu and Sakata, Yoichi},
abstractNote = {Desiccation tolerance is an ancestral feature of land plants and is still retained in non-vascular plants such as bryophytes and some vascular plants. However, except for seeds and spores, this trait is absent in vegetative tissues of vascular plants. Although many studies have focused on understanding the molecular basis underlying desiccation tolerance using transcriptome and proteome approaches, the critical molecular differences between desiccation tolerant plants and non-desiccation plants are still not clear. The moss Physcomitrella patens cannot survive rapid desiccation under laboratory conditions, but if cells of the protonemata are treated by the phytohormone abscisic acid (ABA) prior to desiccation, it can survive 24 h exposure to desiccation and regrow after rehydration. The desiccation tolerance induced by ABA (AiDT) is specific to this hormone, but also depends on a plant transcription factor ABSCISIC ACID INSENSITIVE3 (ABI3). Here we report the comparative proteomic analysis of AiDT between wild type and ABI3 deleted mutant (Δabi3) of P. patens using iTRAQ (Isobaric Tags for Relative and Absolute Quantification). From a total of 1980 unique proteins that we identified, only 16 proteins are significantly altered in Δabi3 compared to wild type after desiccation following ABA treatment. Among this group, three of the four proteins that were severely affected in Δabi3 tissue were Arabidopsis orthologous genes, which were expressed in maturing seeds under the regulation of ABI3. These included a Group 1 late embryogenesis abundant (LEA) protein, a short-chain dehydrogenase, and a desiccation-related protein. Our results suggest that at least three of these proteins expressed in desiccation tolerant cells of both Arabidopsis and the moss are very likely to play important roles in acquisition of desiccation tolerance in land plants. Furthermore, our results suggest that the regulatory machinery of ABA- and ABI3-mediated gene expression for desiccation tolerance might have evolved in ancestral land plants before the separation of bryophytes and vascular plants. - Highlights: • Large-scale proteomics highlighted proteins related to plant desiccation tolerance. • The proteins were regulated by both the phytohormone ABA and ABI3. • The proteins accumulated in desiccation tolerant cells of both Arabidopsis and moss. • Evolutionary origin of regulatory machinery for desiccation tolerance is proposed.},
doi = {10.1016/J.BBRC.2016.02.024},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 471,
place = {United States},
year = {2016},
month = {3}
}