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Title: Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression

Abstract

Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer. - Highlights: • Daphnetin, a coumarin-derivative, inhibited invasion and migration of LM8 cells. • Stress fibers and filopodia were decreased by daphnetin treatment. • Daphnetin decreased RhoA and Cdc42 protein expression.

Authors:
 [1];  [2];  [3];  [1];  [4];  [5];  [6];  [3];  [1];  [1]
  1. Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Kyoto (Japan)
  2. Department of Pharmacognosy, Kyoto Pharmaceutical University, Kyoto (Japan)
  3. Education and Research Center for Clinical Pharmacy, Kyoto Pharmaceutical University, Kyoto (Japan)
  4. Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Kyoto (Japan)
  5. Department of Orthopedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto (Japan)
  6. Department of Biology, Osaka Medical Center of Cancer and Cardiovascular Diseases, Osaka (Japan)
Publication Date:
OSTI Identifier:
22594260
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 471; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL STRESS; CALVES; COUMARIN; DMSO; FIBERS; GUANIDINES; MEMBRANES; METASTASES; OSTEOSARCOMAS; PHOSPHATES; PROTEINS; RIBOSOMAL RNA

Citation Formats

Fukuda, Hiroki, Nakamura, Seikou, Chisaki, Yugo, Takada, Tetsuya, Toda, Yuki, Murata, Hiroaki, Department of Orthopedic Surgery, Matsushita Memorial Hospital, Osaka, Itoh, Kazuyuki, Yano, Yoshitaka, Takata, Kazuyuki, and Ashihara, Eishi. Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.01.179.
Fukuda, Hiroki, Nakamura, Seikou, Chisaki, Yugo, Takada, Tetsuya, Toda, Yuki, Murata, Hiroaki, Department of Orthopedic Surgery, Matsushita Memorial Hospital, Osaka, Itoh, Kazuyuki, Yano, Yoshitaka, Takata, Kazuyuki, & Ashihara, Eishi. Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression. United States. https://doi.org/10.1016/J.BBRC.2016.01.179
Fukuda, Hiroki, Nakamura, Seikou, Chisaki, Yugo, Takada, Tetsuya, Toda, Yuki, Murata, Hiroaki, Department of Orthopedic Surgery, Matsushita Memorial Hospital, Osaka, Itoh, Kazuyuki, Yano, Yoshitaka, Takata, Kazuyuki, and Ashihara, Eishi. 2016. "Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression". United States. https://doi.org/10.1016/J.BBRC.2016.01.179.
@article{osti_22594260,
title = {Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression},
author = {Fukuda, Hiroki and Nakamura, Seikou and Chisaki, Yugo and Takada, Tetsuya and Toda, Yuki and Murata, Hiroaki and Department of Orthopedic Surgery, Matsushita Memorial Hospital, Osaka and Itoh, Kazuyuki and Yano, Yoshitaka and Takata, Kazuyuki and Ashihara, Eishi},
abstractNote = {Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer. - Highlights: • Daphnetin, a coumarin-derivative, inhibited invasion and migration of LM8 cells. • Stress fibers and filopodia were decreased by daphnetin treatment. • Daphnetin decreased RhoA and Cdc42 protein expression.},
doi = {10.1016/J.BBRC.2016.01.179},
url = {https://www.osti.gov/biblio/22594260}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 471,
place = {United States},
year = {Fri Feb 26 00:00:00 EST 2016},
month = {Fri Feb 26 00:00:00 EST 2016}
}