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Title: Vitamin D receptor signaling is required for heart development in zebrafish embryo

Abstract

Vitamin D has been found to be associated with cardiovascular diseases. However, the role of vitamin D in heart development during embryonic period is largely unknown. Vitamin D induces its genomic effects through its nuclear receptor, the vitamin D receptor (VDR). The present study investigated the role of VDR on heart development by antisense-mediated knockdown approaches in zebrafish model system. In zebrafish embryos, two distinct VDR genes (vdra and vdrb) have been identified. Knockdown of vdra has little effect on heart development, whereas disrupting vdrb gene causes various cardiac phenotypes, characterized by pericardial edema, slower heart rate and laterality defects. Depletion of both vdra and vdrb (vdra/b) produce additive, but not synergistic effects. To determine whether atrioventricular (AV) cardiomyocytes are properly organized in these embryos, the expression of bmp4, which marks the developing AV boundary at 48 h post-fertilization, was examined. Notably, vdra/b-deficient embryos display ectopic expression of bmp4 towards the ventricle or throughout atrial and ventricular chambers. Taken together, these results suggest that VDR signaling plays an essential role in heart development. - Highlights: • VDR signaling is involved in embryonic heart development. • Knockdown of vdrb, but not vdra, causes decreased heart rate in zebrafish embryo. • Loss ofmore » vdr results in cardiac laterality defects. • Loss of vdra/b alters atrioventricular boundary formation. • Loss of vdra/b causes abnormal cardiac looping.« less

Authors:
 [1];  [2]
  1. Biology Department, Texas A&M University, College Station, TX77843-3258 (United States)
  2. (Saudi Arabia)
Publication Date:
OSTI Identifier:
22594236
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 470; Journal Issue: 3; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADDITIVES; CARDIOVASCULAR DISEASES; EDEMA; EMBRYOS; FERTILIZATION; GENES; HEART; PHENOTYPE; RECEPTORS; VITAMIN D

Citation Formats

Kwon, Hye-Joo, E-mail: hjkwon@pnu.edu.sa, and Biology Department, Princess Nourah University, Riyadh 11671. Vitamin D receptor signaling is required for heart development in zebrafish embryo. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.01.103.
Kwon, Hye-Joo, E-mail: hjkwon@pnu.edu.sa, & Biology Department, Princess Nourah University, Riyadh 11671. Vitamin D receptor signaling is required for heart development in zebrafish embryo. United States. doi:10.1016/J.BBRC.2016.01.103.
Kwon, Hye-Joo, E-mail: hjkwon@pnu.edu.sa, and Biology Department, Princess Nourah University, Riyadh 11671. Fri . "Vitamin D receptor signaling is required for heart development in zebrafish embryo". United States. doi:10.1016/J.BBRC.2016.01.103.
@article{osti_22594236,
title = {Vitamin D receptor signaling is required for heart development in zebrafish embryo},
author = {Kwon, Hye-Joo, E-mail: hjkwon@pnu.edu.sa and Biology Department, Princess Nourah University, Riyadh 11671},
abstractNote = {Vitamin D has been found to be associated with cardiovascular diseases. However, the role of vitamin D in heart development during embryonic period is largely unknown. Vitamin D induces its genomic effects through its nuclear receptor, the vitamin D receptor (VDR). The present study investigated the role of VDR on heart development by antisense-mediated knockdown approaches in zebrafish model system. In zebrafish embryos, two distinct VDR genes (vdra and vdrb) have been identified. Knockdown of vdra has little effect on heart development, whereas disrupting vdrb gene causes various cardiac phenotypes, characterized by pericardial edema, slower heart rate and laterality defects. Depletion of both vdra and vdrb (vdra/b) produce additive, but not synergistic effects. To determine whether atrioventricular (AV) cardiomyocytes are properly organized in these embryos, the expression of bmp4, which marks the developing AV boundary at 48 h post-fertilization, was examined. Notably, vdra/b-deficient embryos display ectopic expression of bmp4 towards the ventricle or throughout atrial and ventricular chambers. Taken together, these results suggest that VDR signaling plays an essential role in heart development. - Highlights: • VDR signaling is involved in embryonic heart development. • Knockdown of vdrb, but not vdra, causes decreased heart rate in zebrafish embryo. • Loss of vdr results in cardiac laterality defects. • Loss of vdra/b alters atrioventricular boundary formation. • Loss of vdra/b causes abnormal cardiac looping.},
doi = {10.1016/J.BBRC.2016.01.103},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 470,
place = {United States},
year = {2016},
month = {2}
}