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Title: miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN

Abstract

MicroRNAs play important roles in the carcinogenesis of many types of cancers by inhibiting gene expression at posttranscriptional level. However, the roles of microRNAs in hepatocellular carcinoma, are still unclear. Here, we identified that miR-367 promotes hepatocellular carcinoma (HCC) cell proliferation by negatively regulates its target gene PTEN. The expression of miR-367 and PTEN are significantly inverse correlated in 35 HCC patients. In HCC cell line, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-367, while miR-367 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-367 mimics significantly promoted the migration and invasion of HCC cells, whereas miR-367 inhibitors significantly reduced cell migration and invasion. Luciferase assays confirmed that miR-367 directly bound to the 3'untranslated region of PTEN, and western blotting showed that miR-367 suppressed the expression of PTEN at the protein levels. This study indicated that miR-367 negatively regulates PTEN and promotes proliferation and invasion of HCC cells. Thus, miR-367 may represent a potential therapeutic target for HCC intervention. - Highlights: • miR-367 mimics promote the proliferation and invasion of HCC cells. • miR-367 inhibitors inhibit the proliferation and invasion of HCC cells. • miR-367 targets 3′UTR of PTEN in HCC cells. • miR-367more » negatively regulates PTEN in HCC cells.« less

Authors:
 [1];  [2];  [1]
  1. Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou (China)
  2. Gastrointestinal Surgery Department, People's Hospital of Zhengzhou, Zhengzhou (China)
Publication Date:
OSTI Identifier:
22594213
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 470; Journal Issue: 1; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARCINOGENESIS; CELL PROLIFERATION; GENES; HEPATOMAS; LUCIFERASE; PATIENTS

Citation Formats

Meng, Xiangrui, E-mail: mengxiangruibb2008@163.com, Lu, Peng, and Fan, Qingxia. miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN. United States: N. p., 2016. Web. doi:10.1016/J.BBRC.2016.01.025.
Meng, Xiangrui, E-mail: mengxiangruibb2008@163.com, Lu, Peng, & Fan, Qingxia. miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN. United States. doi:10.1016/J.BBRC.2016.01.025.
Meng, Xiangrui, E-mail: mengxiangruibb2008@163.com, Lu, Peng, and Fan, Qingxia. Fri . "miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN". United States. doi:10.1016/J.BBRC.2016.01.025.
@article{osti_22594213,
title = {miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN},
author = {Meng, Xiangrui, E-mail: mengxiangruibb2008@163.com and Lu, Peng and Fan, Qingxia},
abstractNote = {MicroRNAs play important roles in the carcinogenesis of many types of cancers by inhibiting gene expression at posttranscriptional level. However, the roles of microRNAs in hepatocellular carcinoma, are still unclear. Here, we identified that miR-367 promotes hepatocellular carcinoma (HCC) cell proliferation by negatively regulates its target gene PTEN. The expression of miR-367 and PTEN are significantly inverse correlated in 35 HCC patients. In HCC cell line, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-367, while miR-367 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-367 mimics significantly promoted the migration and invasion of HCC cells, whereas miR-367 inhibitors significantly reduced cell migration and invasion. Luciferase assays confirmed that miR-367 directly bound to the 3'untranslated region of PTEN, and western blotting showed that miR-367 suppressed the expression of PTEN at the protein levels. This study indicated that miR-367 negatively regulates PTEN and promotes proliferation and invasion of HCC cells. Thus, miR-367 may represent a potential therapeutic target for HCC intervention. - Highlights: • miR-367 mimics promote the proliferation and invasion of HCC cells. • miR-367 inhibitors inhibit the proliferation and invasion of HCC cells. • miR-367 targets 3′UTR of PTEN in HCC cells. • miR-367 negatively regulates PTEN in HCC cells.},
doi = {10.1016/J.BBRC.2016.01.025},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 470,
place = {United States},
year = {Fri Jan 29 00:00:00 EST 2016},
month = {Fri Jan 29 00:00:00 EST 2016}
}